Most people are born with the ability to digest lactose, major carbohydrate in milk and main source of nutrition until weaning. Approximately 75% world's population loses this at some point, while others can lactose into adulthood. This review discusses lactase-persistence alleles that have arisen different populations around world, diagnosis intolerance, its symptomatology management.

The treatment for the eradication of Helicobacter pylori (H. pylori) is complex; full effectiveness rarely achieved and it has many adverse effects. In developing countries, increased resistance to antibiotics its cost make more difficult. Probiotics can reduce effects improve infection efficacy. If first-line therapy fails a second-line using tetracycline, furazolidone proton-pump inhibitors been effective low in Brazil; however implies lot aim this study was minimize increase rate applying...

The clinical importance of preoperative serum levels CA 72-4, carcinoembryonic antigen (CEA), 19-9, and alpha-fetoprotein (AFP) was prospectively evaluated in 44 patients with gastric cancer.The tumor marker were determined by commercial radioimmunoassay kits. Positivity for 72-4 (>4 U/mL), CEA (>5 ng/mL), 19-9 (>37 AFP (>10 ng/mL) correlated according to the stage, histology, lymph node metastasis.CA showed a higher positivity rate cancer (47.7%) than (25%), (0%). combination increased...

The purpose of this study was to verify whether the presence any Helicobacter pylori cag PAI genes or segments – cagA , promoter, cagE cagM tnpB tnpA cagT and left end II (LEC) region would be a useful marker for risk peptic ulcer disease development. H. DNA extracted from positive urease tests 150 patients 65 dyspeptic controls analysed by PCR. Duodenal ulcers were present in 110, gastric 23 both duodenal 17 patients. A significant association ( P <0.001) found between conserved (34 %)....

ABSTRACT Background. The bab A2 gene, which encodes a blood‐group antigen‐binding adhesin that mediates attachment of Helicobacter pylori to human Lewis b antigens on gastric epithelial cells, has been associated with higher risk peptic ulcer and cancer. purpose this study was ascertain the frequency genotype in H. strains patients correlate other virulence factors. Materials Methods. vac A , cag A, genotypes were determined by using polymerase chain reaction (PCR). DNA extracted from...

The aim of this study was to validate the rapid lateral flow Helicobacter pylori stool antigen test (One step H. test, ACON laboratories, San Diego, USA; Prime diagnostics, São Paulo), using 13C-Urea Breath Test as gold standard for infection diagnosis. A total 98 consecutive patients, asymptomatic or dyspeptic, entered study. Sixty-nine were women, with a mean age 45.76 ± 14.59 years (14 79 years). In pylori-positive group, detected in 44 50 positive patients (sensitivity 88%; 95% CI:...

Adult-type hypolactasia, the physiological decline of lactase some time after weaning, was previously associated with LCT -13910C>T polymorphism worldwide except in Africa. Lactase non-persistence is most common phenotype humans, northwestern Europe its long history pastoralism and milking. We had shown association adult-type hypolactasia Brazilians; thus, we assessed frequency among different Brazilian ethnic groups. investigated ethnicity-related this 567 Brazilians [mean age, 42.1 ± 16.8...

Introduction: Helicobacter pylori infection is very prevalent in Brazil, infecting almost 65% of the population. The aim this study was to evaluate presence bacterium oral cavity patients with functional dyspepsia (epigastric pain syndrome), establish main sites mouth, and assess frequency cag A vac genotypes H. . Methods: All 43 outpatients epigastric syndrome, who entered study, were submitted upper gastrointestinal endoscopy rule out organic diseases. stomach confirmed by a rapid urease...

The Helicobacter pylori reinfection seems to be higher in developing countries, than developed ones. aim of the study was determine annual recurrence rate H. pylori, Brazilian patients with peptic ulcer disease, a 5-year follow-up.Patients, disease diagnosed by upper digestive endoscopy (UDE) and infection verified histological analysis, rapid urease test, polymerase chain reaction, urea breath test (UBT), were treated for bacterial eradication. cure using same tests, 3 months after....

Adult type hypolactasia, the genetically programmed down-regulation of lactase enzyme activity in intestinal wall after weaning, is a common condition worldwide, except for northwestern Europe, where prevalence less than 10%.Lactose intolerant individuals complain abdominal cramps, bloating, distention, flatulence and diarrhea milk or lactose-containing food ingestion. 1he diagnosis adult-type hypolactasia can be achieved by hydrogen breath test that cumbersome provokes symptoms, more...

H. pylori seroprevalence in Brazilians varies and is dependent on socioeconomic status, sanitation conditions ethnicity; furthermore, not always associated with the incidence of gastric cancer, suggesting role more virulent strains. The purpose this study was to analyze association strains cancer.DNA extracted from biopsies thirty-four cases cancer (11 intestinal-type, 23 diffuse-type), patients endoscopic gastritis. presence cagPAI genes (cagA, cagA promoter, cagE, cagM, tnpB, tnpA, cagT...

CONTEXT: In spite of Helicobacter pylori infection being the etiological cause peptic ulcer and its high prevalence in Brazil, disease has been poorly studied. OBJECTIVES: To verify patients a tertiary care hospital. METHODS: Diagnostic findings from 1,478 consecutive endoscopies were correlated with urease test results for H. diagnosis demographic data total 3,779 performed 2005. The mean age was 51.14 ± 16.46, 613 (41.5%) men. RESULTS: Peptic diagnosed 494 (33.4%) 54.86 14.53, 205 (52%)...

Fecal elastase is a noninvasive test for pancreatic insufficiency diagnosis.Evaluate the usefulness of fecal 1 indication exocrine among former alcohol addicts and patients with chronic pancreatitis.Forty-three pancreatitis thirty-three asymptomatic entered study. The levels were measured using commercial kit. Pancreatic imaging findings used to categorize groups.The significantly lower in than group tissue calcifications, duct alterations, or atrophy. With cutoff level 100 μg/g, sensitivity...

Inactivation of tumor suppressor genes has been frequently observed in gastric carcinogenesis. Our purpose was to study the involvement p53, APC, DCC, and Rb carcinoma. METHOD: Loss heterozygosity DCC studied 22 cancer tissues using polymerase chain reaction; single-strand conformation polymorphism p53 gene exons 5-6 7-8 35S-dATP, expression detected a histological immunoperoxidase method with an anti-p53 clone. RESULTS AND DISCUSSION: No loss any these genes; homozygous deletion 23% (3/13)...

Aim . To compare 10-day standard triple therapy versus sequential as first-line treatment in patients infected with H. pylori Methods One hundred positive (diagnosed by rapid urease test and histology), average age of 47.2, M/F = 28/72, were randomized to receive either (TT) follows: lansoprazole 30 mg, clarithromycin 500 amoxicillin 1 g, b.i.d. for ten days, or (ST) placebo 1.0 g b.i.d the first five followed tinidazole mg b.i.d, remaining days. Eradication rates determined 60 days after...

The Brazilian consensus recommends a short-term treatment course with clarithromycin, amoxicillin and proton-pump inhibitor for the eradication of Helicobacter pylori (H. pylori). This has good efficacy, but cannot be afforded by large part population. Azithromycin, omeprazole are subsidized, several aims, federal government. Therefore, that uses these drugs is low-cost one, its efficacy regarding bacterium yet to demonstrated. study's purpose was verify H. in infected patients who presented...

PURPOSE: To analyze the usefulness of Quick Lactose Intolerance Test in relation to genetic test based on LCT-13910C>T genotypes, previously validated for clinical practice, primary hypolactasia/lactase-persistence diagnosis. METHODS: Thirty-two dyspeptic patients that underwent upper gastrointestinal endoscopy entered study. Two postbulbar duodenal biopsies were taken test, and gastric antral biopsy DNA extraction polymorphism analysis. was also extracted from after being used kept...

It has been proposed that the combination of gastrin-17 (G-17), pepsinogens I and II (PGI PGII), anti-Helicobacter pylori (H. pylori) antibodies (GastroPanel®, BIOHIT HealthCare, Helsinki, Finland) could serve as biomarkers atrophic gastritis.This study aimed to ensure diagnostic accuracy GastroPanel® evaluate effect proton pump inhibitors (PPIs) on these biomarkers.Dyspeptic patients who underwent gastrointestinal endoscopy were enrolled in present study. Histological findings, which gold...

ABSTRACT BACKGROUND: Rectal bleeding is the most important symptom of intestinal neoplasia; thus, tests occult blood detection in stools are widely used for pre neoplastic lesions and colorectal cancer (CRC) screening. OBJECTIVE: Evaluate accuracy OC-Sensor quantitative test (Eiken Chemical, Tokyo, Japan) at cut-off 10 µg Hb/g feces (50 ng/mL) a cohort subjects that had to undergo diagnostic colonoscopy, if more than one sample collected consecutive days would improve test. METHODS: Patients...

ContextOne of the limitations 13C-urea breath test forHelicobacter pylori infection diagnosis in Brazil is substrate acquisition capsule presentation.ObjectivesThe purpose this study was to evaluate a capsule-based13C-urea, manipulated by Pharmacy Division, for clinical practice.MethodsFifty patients underwent conventional and test. Samples were collected at baseline after 10, 20 30 minutes ingestion. Urease histology used as gold standard 83 patients.ResultsIn total 50 patients, 17 positive...

Objective: To verify whether the variable number of tandem repeat (VNTR) polymorphism in IL-1RN gene that encodes interleukin (IL)-1 receptor antagonist (IL-1Ra) plays a role outcome gastrointestinal diseases associated with Helicobacter pylori ( H. ) infection. Methods: Patients normal endoscopy (n = 71), inflammation upper tract only 196), gastric ulcer 28), duodenal 76), and cancer 19) were studied. infection was diagnosed by urease test, histological examination, polymerase chain...