A5039753496- Forest Insect Ecology and Management
- Insect and Pesticide Research
- Plant biochemistry and biosynthesis
- Insect-Plant Interactions and Control
- Insect and Arachnid Ecology and Behavior
- Insect Resistance and Genetics
- Plant and animal studies
- Neurobiology and Insect Physiology Research
- Entomopathogenic Microorganisms in Pest Control
- Insect Pheromone Research and Control
- Microbial Natural Products and Biosynthesis
- Fish Ecology and Management Studies
- Natural product bioactivities and synthesis
- Forest Ecology and Biodiversity Studies
- Plant Gene Expression Analysis
- Coleoptera Taxonomy and Distribution
- Hemiptera Insect Studies
- Isotope Analysis in Ecology
- Plant Reproductive Biology
- Phytochemical compounds biological activities
- Yeasts and Rust Fungi Studies
- Plant Pathogens and Fungal Diseases
- Bee Products Chemical Analysis
- Biochemical and biochemical processes
- Sesquiterpenes and Asteraceae Studies
Natural Resources Canada
2019-2024
Canadian Forest Service
2019-2024
Université Laval
2020-2022
Forestry Research Centre
2021
University of Alberta
2021
University of British Columbia
2010-2019
Canada's Michael Smith Genome Sciences Centre
2010-2019
Centre Intégré de Santé et de Services Sociaux des Laurentides
2019
University of Wisconsin–Oshkosh
2019
Simon Fraser University
1999-2018
Summary Terpenoids (isoprenoids) encompass more than 40 000 structures and form the largest class of all known plant metabolites. Some terpenoids have well‐characterized physiological functions that are common to most species. In addition, many structurally diverse may function in taxonomically discrete, specialized interactions with other organisms. Historically, terpenoids, together alkaloids phenolics, been referred as secondary More recently, these compounds become widely recognized,...
Abstract White spruce (Picea glauca) is a dominant conifer of the boreal forests North America, and providing genomics resources for this commercially valuable tree will help improve forest management conservation efforts. Sequencing assembling large highly repetitive genome though pushes boundaries current technology. Here, we describe whole-genome shotgun sequencing strategy using two Illumina platforms an assembly approach ABySS software. We report 20.8 giga base pairs draft in 4.9...
The mountain pine beetle, Dendroctonus ponderosae Hopkins, is the most serious insect pest of western North American forests. A recent outbreak destroyed more than 15 million hectares forests, with major environmental effects on forest health, and economic industry. has in part been driven by climate change, will contribute to increased carbon emissions through decaying We developed a genome sequence resource for beetle better understand unique aspects this insect's biology. draft de novo...
The European spruce bark beetle, Ips typographus, and the North American mountain pine Dendroctonus ponderosae (Coleoptera: Curculionidae: Scolytinae), are severe pests of coniferous forests. Both beetle species utilize aggregation pheromones to coordinate mass-attacks on host trees, while odorants from non-host trees modulate pheromone response. Thus, olfactory sense is utmost importance for fitness. However, information genes underlying detection has been lacking in beetles limited...
White spruce (Picea glauca), a gymnosperm tree, has been established as one of the models for conifer genomics. We describe draft genome assemblies two white genotypes, PG29 and WS77111, innovative tools assembly very large genomes, genomics resources developed in this process. The genotypes originate from distant geographic regions western (PG29) eastern (WS77111) North America, represent elite trees Canadian tree-breeding programs. present an update (V3 V4) previously reported V2 introduce...
The honey bee queen produces pheromones that function in both releaser and primer roles such as attracting a retinue of workers around her, drones on mating flights, preventing from reproducing at the individual (worker egg-laying) colony (swarming) level, regulating several other aspects functioning. mandibular pheromone (QMP), consisting five synergistic components, is only chemically identified (Apis mellifera L.) queen, but this does not fully duplicate pheromonal activity full extract....
The diversity of terpenoid compounds produced by plants plays an important role in mediating various plant-herbivore, plant-pollinator, and plant-pathogen interactions. This has resulted from gene duplication neofunctionalization the enzymes that synthesize subsequently modify terpenes. Two diterpene synthases Norway spruce (Picea abies), isopimaradiene synthase levopimaradiene/abietadiene synthase, provide hydrocarbon precursors for most resin acids found defensive oleoresin conifers....
In conifers, terpene synthases (TPSs) of the gymnosperm-specific TPS-d subfamily form a diverse array mono-, sesqui-, and diterpenoid compounds, which are components oleoresin secretions volatile emissions. These compounds contribute to defence against herbivores pathogens perhaps also protect abiotic stress.The availability extensive transcriptome resources in expressed sequence tags (ESTs) full-length cDNAs several spruce (Picea) species allowed us estimate that conifer genome contains at...
Sandalwood oil is one of the world’s most highly prized essential oils, appearing in many high-end perfumes and fragrances. Extracted from mature heartwood several Santalum species, sandalwood comprised mainly sesquiterpene olefins alcohols. Four sesquiterpenols, α-, β-, epi-β-santalol α-exo-bergamotol, make up approximately 90% album. These compounds are hydroxylated analogues epi-β-santalene α-exo-bergamotene. By mining a transcriptome database S. album for candidate cytochrome P450 genes,...
The mountain pine beetle (Dendroctonus ponderosae; MPB) is an eruptive bark species affecting forests of western North America. MPB are exposed to volatile monoterpenes, which important host defense chemicals. We assessed the toxicity ten most abundant monoterpenes lodgepole (Pinus contorta), a major in current epidemic, against adult from two locations British Columbia, Canada. Monoterpenes were tested as individual volatiles and included (-)-β-phellandrene, (+)-3-carene, myrcene,...
Olfaction and gustation underlie behaviors that are crucial for insect fitness, such as host mate selection. The detection of semiochemicals is mediated via proteins from large rapidly evolving chemosensory gene families; however, the links between a species' ecology diversification these genes remain poorly understood. Hence, we annotated genomes select wood-boring coleopterans, compared repertoires stenophagous species with those polyphagous species.We 86 odorant receptors (ORs), 60...
Abstract The biosynthesis of the tetracyclic diterpene ent-kaurene is a critical step in general (primary) metabolism gibberellin hormones. ent-Kaurene formed by two-step cyclization geranylgeranyl diphosphate via intermediate ent-copalyl diphosphate. In lower land plant, moss Physcomitrella patens, single bifunctional synthase (diTPS) catalyzes both steps. contrast, angiosperms, two consecutive cyclizations are catalyzed distinct monofunctional enzymes, (CPS) and (KS). enzyme, or...
Summary Conifers are extremely long‐lived plants that have evolved complex chemical defenses in the form of oleoresin terpenoids to resist attack from pathogens and herbivores. In these species, terpenoid diversity is determined by size composition terpene synthase (TPS) gene family single‐ multi‐product profiles enzymes. The monoterpene (+)‐3‐carene associated with resistance Sitka spruce ( Picea sitchensis ) white pine weevil Pissodes strobi ). We used a combined genomic, proteomic...
The mountain pine beetle (MPB; Dendroctonus ponderosae Hopkins), a major forest pest native to western North America, has extended its range north and eastward during an ongoing outbreak. Determining how the MPB expanded breach putative barriers, whether physical (nonforested prairie high elevation of Rocky Mountains) or climatic (extreme continental climate where temperatures can be below −40 °C), may contribute our general understanding changes as well management current epidemic. Here, we...
Abstract Background The mountain pine beetle ( Dendroctonus ponderosae ) is a significant coniferous forest pest in western North America. It relies on aggregation pheromones to colonize hosts. Its three major pheromone components, trans -verbenol, exo -brevicomin, and frontalin, are thought arise via different metabolic pathways, but the enzymes involved have not been identified or characterized. We produced ESTs from male female midguts associated fat bodies used custom oligonucleotide...
Mountain pine beetles, Dendroctonus ponderosae Hopkins (Coleoptera: Curculionidae), are native to western North America, but have recently begun expand their range across the Canadian Rocky Mountains. The requirement for larvae withstand extremely cold winter temperatures and potentially toxic host secondary metabolites in midst of ongoing development makes this a critical period lives.We uncovered global protein profiles overwintering mountain beetle larvae. We also quantitatively compared...
The levopimaradiene/abietadiene synthase from Norway spruce (<i>Picea abies</i>; PaLAS) has previously been reported to produce a mixture of four diterpene hydrocarbons when incubated with geranylgeranyl diphosphate as the substrate: levopimaradiene, abietadiene, neoabietadiene, and palustradiene. However, variability in assay products observed by GC-MS this orthologous conifer synthases over past 15 years suggested that these diterpenes may not be initial enzyme but are rather dehydration...
Abstract Background The mountain pine beetle (MPB, Dendroctonus ponderosae ) epidemic has affected lodgepole ( Pinus contorta across an area of more than 18 million hectares forests in western Canada, and is a threat to the boreal jack banksiana forest. Defence pines against MPB associated fungal pathogens, as well other pests, involves oleoresin monoterpenes, which are biosynthesized by families terpene synthases (TPSs). Volatile monoterpenes also serve host recognition cues for precursors...