A5090984283- Neuropeptides and Animal Physiology
- Hypothalamic control of reproductive hormones
- Receptor Mechanisms and Signaling
- Sperm and Testicular Function
- Neuroendocrine regulation and behavior
- Ion channel regulation and function
- Reproductive Biology and Fertility
- Ovarian function and disorders
- Asthma and respiratory diseases
- Marine Toxins and Detection Methods
- Nitric Oxide and Endothelin Effects
- Estrogen and related hormone effects
- Neuroscience of respiration and sleep
- Pharmacological Effects and Assays
- Cancer, Stress, Anesthesia, and Immune Response
- Reproductive System and Pregnancy
- Respiratory and Cough-Related Research
- Pharmacological Receptor Mechanisms and Effects
- Peptidase Inhibition and Analysis
- Hormonal and reproductive studies
- Hormonal Regulation and Hypertension
- Synthesis and Biological Evaluation
- Prenatal Screening and Diagnostics
- Pancreatic function and diabetes
- Cardiac electrophysiology and arrhythmias
Instituto de Investigaciones Químicas
2014-2024
Centro de Investigaciones Científicas Isla de la Cartuja
2002-2024
Universidad de Sevilla
2007-2016
Consejo Superior de Investigaciones Científicas
1998-2016
IVI Sevilla Clinic
2007-2008
Esteve Química (Spain)
2006
Instituto de Química Orgánica General
2006
Hôpital Européen Georges-Pompidou
2005
Universidad de La Laguna
1992-2002
Hospital San Agustin
1995-2001
Kisspeptin, the product of KISS1 gene, plays an essential role in regulation spermatogenesis acting primarily at hypothalamic level gonadotropic axis. However, presence kisspeptin and its canonical receptor, KISS1R, spermatozoa has not been explored nor direct effects on sperm function have studied so far. In present study, we analysed expression receptor cells by western blot immunocytochemistry assays evaluated exposure to intracellular Ca(2+) concentration, [Ca(2+)]i, motility,...
Are neurokinin B (NKB), NK3 receptor (NK3R), kisspeptin (KISS1) and (KISS1R) expressed in human ovarian granulosa cells? The NKB/NK3R kisspeptin/KISS1R systems are co-expressed functionally active cells. KISS1/KISS1R essential for reproduction. In addition to their well-recognized role hypothalamic neurons, these peptide may contribute the control of fertility by acting directly on gonads, but such a direct gonadal remains largely unknown. This study analyzed matched mural cells (MGCs)...
Tachykinins may be involved in reproduction. A reverse transcription-polymerase chain reaction assay was used to analyze the expression of tachykinins and tachykinin receptors different types reproductive cells from mice. The preprotachykinin (PPT) genes, PPT-A, PPT-B PPT-C, that encode substance P/neurokinin A, neurokinin B, hemokinin-1, respectively, genes NK1, NK2, NK3 were all expressed, at levels, uterus superovulated, unfertilized mRNA neprilysin (NEP), main enzyme metabolism, also...
Studies were undertaken to determine the nature of receptors mediating contractile effects tachykinins in uteri nonpregnant women, and analyse expression preprotachykinins (PPT), tachykinin cell‐surface peptidase, neprilysin (NEP), myometrium from pregnant women. The neurokinin B (NKB) precursor PPT‐B was expressed higher levels than Faint PPT‐A mRNA detectable but not PPT‐C, gene encoding novel peptide hemokinin‐1 (HK‐1), present trace amounts both Tachykinin NK 2 more strongly tissues 1...
The presence of CB1 and CB2 cannabinoid receptors their physiological role in the kidney has been described animal models but not humans. Our aim this study was to evaluate these human kidney, adult fetal. For purpose, RT-PCR, western-blot immunohisto-chemical assays were performed. RT-PCR confirmed receptor mRNA absence fetal kidney. Western-blot immunohistochemical revealed protein, which displayed a similar distribution kidneys. Proximal distal convoluted tubule cells intercalated...
G-protein coupled receptors (GPCRs) belong to the seven transmembrane receptor superfamily that transduce signals via G proteins in response external stimuli initiate different intracellular signaling pathways which culminate specific cellular responses. The expression of diverse GPCRs at plasma membrane human spermatozoa suggests their involvement regulation sperm fertility. However, events downstream many remain uncharacterized. Here, we selected kappa-opioid (KOR) as a study model and...
Tachykinin neuropeptides, such as substance P, are localized to a population of sensory fibers that innervate the mammalian female reproductive tract. In present study, we have characterized tachykinin NK1 receptor (NK1R), NK2 (NK2R), and NK3 (NK3R) gene expression by semiquantitative RT-PCR in uteri from ovariectomized rats studied their regulation response 17β-estradiol (E2), progesterone (P4), or combination both. addition, analyzed neutral endopeptidase 24.11 (NEP), most important enzyme...
The effects of the β 2 ‐adrenoceptor agonists, salbutamol and formoterol, on increase microvascular permeability induced by histamine or bradykinin in guinea‐pig airways have been studied vivo. Extravasation intravenously injected Evans blue dye was used as an index permeability. formoterol pulmonary airway resistance also studied. totally inhibited formoterol. ED 50 two mediators were 0.59 ± 0.21 ( n = 5) 0.20 0.14 μg kg −1 respectively for salbutamol, 0.13 0.12 6) 0.02 0.01 Salbutamol (10...
Abstract Background Cell-surface glycoproteins play critical roles in cell-to-cell recognition, signal transduction and regulation, thus being crucial cell proliferation cancer etiogenesis development. DPP IV NEP are ubiquitous glycopeptidases closely linked to tumor pathogenesis development, they used as markers some cancers. In the present study, activity protein mRNA expression of these were analysed a subset clear-cell (CCRCC) chromophobe (ChRCC) renal carcinomas, oncocytomas (RO)....
Abstract The mechanism of relaxant activity six benzylisoquinolines was examined in order to determine the minimal structural requirements that enable these compounds have either a non-specific action like papaverine or an inhibitory on calcium entry via potential-operated channels. All alkaloids tested totally partially relaxed KCl-depolarized rat uterus and inhibited oxytocin-induced rhythmic contractions. Only glaucine laudanosine K+-induced uterine contractions more than In Ca+-free...
Tachykinins and tachykinin receptors are widely distributed in the male reproductive tract appear to be involved reproduction. However, function expression of tachykinins their human spermatozoa remain poorly studied. We analysed effects on sperm motility characterized population spermatozoa. Motility analysis was performed following World Health Organization guidelines we found that substance P (SP), hemokinin-1 (hHK-1), neurokinin A (NKA) B (NKB) produced concentration-dependent increases...
Abstract Background We examined the presence and function of tachykinins tachykinin-degrading enzymes neprilysin (NEP) neprilysin-2 (NEP2) in human spermatozoa. Methods Freshly ejaculated semen was collected from forty-eight normozoospermic donors. analyzed expression substance P, neurokinin A, B, hemokinin-1, NEP NEP2 sperm cells by reverse-transcriptase polymerase chain reaction (RT-PCR), western blot immunocytochemistry assays evaluated effects inhibitor phosphoramidon on motility absence...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTNorstephalagine et Atherospermidine, Deux Aporphines d'Artabotrys maingayi Relaxantes du Muscle LisseDiego Cortes, M. Yolanda Torrero, Pilar D'Ocon, Luz Candenas, André Cavé, and A. Hamid HadiCite this: J. Nat. Prod. 1990, 53, 2, 503–508Publication Date (Print):March 1, 1990Publication History Published online1 July 2004Published inissue 1 March 1990https://pubs.acs.org/doi/10.1021/np50068a039https://doi.org/10.1021/np50068a039research-articleACS...