A5054870381- Cancer Cells and Metastasis
- Cancer-related Molecular Pathways
- Breast Cancer Treatment Studies
- Endometriosis Research and Treatment
- Advanced Breast Cancer Therapies
- Women's cancer prevention and management
- Metabolism, Diabetes, and Cancer
- Cancer Genomics and Diagnostics
- Cancer-related molecular mechanisms research
- HER2/EGFR in Cancer Research
- Cancer Risks and Factors
- MicroRNA in disease regulation
- RNA modifications and cancer
- Biomarkers in Disease Mechanisms
- Hepatitis B Virus Studies
- Cell Adhesion Molecules Research
- Molecular Biology Techniques and Applications
- CAR-T cell therapy research
- Vitamin D Research Studies
- Estrogen and related hormone effects
- TGF-β signaling in diseases
- Hepatitis C virus research
- Thyroid Cancer Diagnosis and Treatment
- Receptor Mechanisms and Signaling
- Reproductive System and Pregnancy
Universidade Federal de São Paulo
2014-2016
Universidade de São Paulo
2006-2015
Fundação de Apoio à Universidade Federal de São Paulo
2013
Colsan - Associação Beneficente de Coleta de Sangue
2012
Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo
2004-2012
Instituto Brasileiro de Controle do Câncer
2006
The importance of epithelial-stroma interaction in normal breast development and tumor progression has been recognized. To identify genes that were regulated by these reciprocal interactions, we cocultured a nonmalignant (MCF10A) cancer derived (MDA-MB231) basal cell lines, with fibroblasts isolated from benign-disease adjacent tissues (NAF) or carcinoma-associated (CAF), transwell system. Gene expression profiles each coculture pair compared the correspondent monocultures, using customized...
Cancer-associated fibroblasts (CAF) influence tumor development at primary as well in metastatic sites, but there have been no direct comparisons of the transcriptional profiles stromal cells from different sites. In this study, we used customized cDNA microarrays to compare gene expression profile (CAF, n = 4), lymph node metastasis (N+, 3) and bone marrow (BM, 4) obtained breast cancer patients. Biological validation was done another 16 samples by RT-qPCR. Differences between CAF vs N+, BM...
Our aim was to evaluate the interaction between breast cancer cells and nodal fibroblasts, by means of their gene expression profile. Fibroblast primary cultures were established from negative positive lymph nodes patients a similar pattern identified, following cell culture. Fibroblasts (MDA-MB231, MDA-MB435, MCF7) cultured alone or co-cultured separated porous membrane (which allows passage soluble factors) for comparison. Each lineage exerted particular effect on fibroblasts viability...
The importance of tumor–stromal cell interactions in breast tumor progression and invasion is well established. Here, an evaluation differential genomic profiles carcinoma-associated fibroblasts (CAFs) compared to derived from tissues adjacent fibroadenomas (NAFs) revealed altered focal adhesion pathways. These data were validated through confocal assays. To verify the possible role lymph node invasion, we constructed a tissue microarray consisting primary cancer samples corresponding...
Chronic hepatitis C (CHC) has emerged as a leading cause of cirrhosis in the U.S. and across world. To understand role apoptotic pathways virus (HCV) infection, we studied mRNA protein expression patterns apoptosis-related genes peripheral blood mononuclear cells (PBMC) obtained from patients with HCV infection. The present study included 50 subjects which plasma samples were positive for HCV, but negative human immunodeficiency (HIV) or B (HBV). These cases divided into four groups...
Abstract The importance of tumor-stromal cells interactions in breast tumor progression and invasion has been recognized; therefore modifications the stromal fibroblasts can play a significant role overall cancer development progression, presence microenvironment metastatic lymph nodes was described, suggesting its process. Here after having evaluated differential genomic profile carcinoma associated (CAFs) when compared to derived from tissues adjacent fibroadenomas (NAFs), we depicted...
10570 Background: In breast cancer (BC), stromal-epithelial cell interactions may influence tumor development in the primary (PT) and metastatic sites, which be reflected by a differential phenotype of malignant cells fibroblasts (Fb). Methods: To investigate role stromal site lymph nodes (LN), culture Fb obtained from tissue: PT (n = 4) or peritumoral 4); LN: involved (iLN, n 3) uninvolved BC patients, were established. normal (MCF10A) (MDA-MB231) cultured isolated co-cultured separated...
Abstract The importance of tumor-stromal cells interactions in breast tumor progression and invasion has been recognized; therefore, modifications the stromal fibroblasts can play a significant role overall cancer development progression. Accumulating evidence indicates that epigenetic phenomena such as microRNAs are extremely important were already recognized key regulators gene expression, well expression these small RNAs linked to human disease, including those related estrogen-dependent...
Methods Fibroblasts primary culture was established from 11 breast cancer patients. Expression analysis evaluated in PT (n=4), N+ (n=3) and BM (n=4) through a customized cDNA microarray platform (4,800 ORESTES) analyzed by SAM (TMEV; FDR 0%) functional performed using DAVID. Technical validation 6 samples biological fibroblasts obtained others 25 patients as RT-qPCR.
Endometriosis seems to be a precursor of epithelial ovarian cancer, especially clear cell and endometrioid adenocarcinomas. The features shared by endometriosis cancer include the ability evade apoptosis, stem cell‐like angiogenic potential. As such characteristics are encoded cell's genetic constitution; acquired mutations responsible for malignant transformation endometriosis. So, number differences between found at molecular level miRNAs recognized as key regulators gene expression....
Abstract Background: In breast cancer, there is increasing evidence that stromal cells, including fibroblasts, may influence tumor development in the primary site, as well regional and distant metastatic sites. Objectives: To compare fibroblasts from (PT), lymph nodes bone marrow (BM) cancer patients, by means of their expression profile. Methods: Fibroblasts culture was established 14 patients: PT (n=4), axillary (3 positive 3 negative nodes) BM (2 with 2 without micrometastasis, identified...