Herein, a portable lab-in-a-syringe device integrated with smartphone sensing platform was designed for rapid, visual quantitative determination of organophosphorus pesticides (OPs) via colorimetric and fluorescent signals. The chiefly made up conjugate pad labeled cetyltrimethylammonium bromide-coated gold nanoparticles (CTAB-Au NPs) modified by ratiometric probes (red-emission quantum dots@SiO2 nanoparticles@green-emission dots, rQDs@SiO2@gQDs probe), which assembled through disposable...

Particulate matter, especially PM2.5, can invade the central nervous system (CNS) via olfactory pathway to induce neurotoxicity. The bulb (OB) is key component integrating immunoprotection and olfaction processing necessarily involved in relevant CNS health outcomes. Here we show that a microglial chemokine receptor, CCR5, target of environmentally PM2.5 OB trigger neuroinflammation then neuropathological injuries. Mechanistically, PM2.5-induced CCR5 upregulation results pro-inflammatory...

The mitochondrial ATP synthase is a multimeric enzyme complex with an overall molecular weight of about 600,000 Da. motor composed two separable parts: F1 and Fo. portion contains the catalytic sites for synthesis protrudes into matrix. Fo forms proton turbine that embedded in inner membrane connected to rotor F1. flux protons flowing down potential gradient powers rotation driving ATP. Thus, flow though coupled This review will discuss structure/function relationship as determined by...

Abstract Background Qingcaosha Reservoir is China’s largest river impoundment and an important drinking water resource for Shanghai city. Although antibiotics contamination in the reservoir has been reported, little known about presence of antibiotic resistance genes (ARGs). In this study, occurrence distribution 12 ARGs class 1 integron gene ( int I-1) sediments collected from were investigated year. Results The detected both sediment samples, no significant temporal or spatial variations...

The vacuolar (H+)-ATPases (or V-ATPases) are structurally related to the F1F0ATP synthases of mitochondria, chloroplasts and bacteria, being composed a peripheral (V1) an integral (V0) domain. To further investigate arrangement subunits in V-ATPase complex, covalent cross-linking has been carried out on from clathrin-coated vesicles using three different reagents. Cross-linked products were identified by molecular weight Western blot analysis polyclonal antibodies raised against individual...

Toxicology studies heavily rely on morphometric analysis to detect abnormalities and diagnose disease processes. The emergence of ever-increasing varieties environmental pollutants makes it difficult perform timely assessments, especially using in vivo models. Herein, we propose a deep learning-based (DLMA) quantitatively identify eight abnormal phenotypes (head hemorrhage, jaw malformation, uninflated swim bladder, pericardial edema, yolk bent spine, dead, unhatched) vital organ features...

A SERS microarray chip was prepared to achieve the detection of DR biomarkers miR-21 and miR-152 using a competitive recognition strategy.

Recent advancements in large language models (LLMs) have demonstrated their impressive abilities various reasoning and decision-making tasks. However, the quality coherence of process can still benefit from enhanced introspection self-reflection. In this paper, we introduce Multiplex CoT (Chain Thought), a method that enables LLMs to simulate form self-review while reasoning, by initiating double Chain Thought (CoT) thinking. leverages power iterative where model generates an initial chain...

This report details Lumen Labs' novel approach to processing Social Networking Service (SNS) data. We leverage knowledge distillation, specifically a simple distillation method inspired by DeepSeek-R1's CoT acquisition, combined with prompt hacking, extract valuable training data from the Grok model. is then used fine-tune Phi-3-mini model, augmented mask-like mechanism designed for handling nuances of SNS Our demonstrates state-of-the-art (SOTA) performance on several tasks, outperforming...

A significant correlation between the relative abundances of ARGs and MGEs in a swine farm its surrounding environments was found.

Diminished ovarian reserve (DOR), a major cause of female infertility, is closely linked to insulin resistance (IR). Traditional Chinese Medicine (TCM) approaches, such as the Gengnianchun (GNC) formula, focus on restoring function by improving IR and regulating hormonal balance. Despite GNC's demonstrated efficacy, its precise therapeutic mechanisms remain unclear. This study aims elucidate which GNC ameliorates IR-induced DOR through comprehensive pharmacological experimental validation....

Accumulating evidence suggests a strong correlation between air pollution and neurological disorders; however, appropriate models methodologies are currently lacking. In this study, human nasal RPMI 2650 cell model based on air-liquid interface culture was discovered to possess olfactory epithelial cells. Two short-chain per- polyfluoroalkyl substances (PFAS), PFBA PFHxA, were used validate the performance of model. RNA sequencing initially revealed adverse effects two PFAS at...

2,2′,4,4′-Tetrabromodiphenyl ether (BDE47) is a prevalent environmental pollutant and has been demonstrated to be serious toxicant in both humans animals, but little known about the molecular mechanism underlying its toxic effect on early development of vertebrates. BDE47-treated zebrafish larvae were found present light-related locomotion reduction our previous study, therefore, we aimed use high throughput sequencing investigate possible reasons from transcriptomic perspective. By exposing...

Our published studies have revealed that 2,2′,4,4′-tetrabromodiphenyl ether (BDE-47) could disrupt retina morphologies and related gene expressions of zebrafish larvae. Its possible effects on fish vision needed to be uncovered since sensory systems, especially eyes, are vital wildlife. In this paper, two tests for development (opsin expression photoreceptor immunostaining) visually guided behaviors (optokinetic response looming-evoked escape) were designed investigate the potential visual...