A5068311311- Cancer, Hypoxia, and Metabolism
- Diet and metabolism studies
- Cellular Mechanics and Interactions
- Neurological disorders and treatments
- Tissue Engineering and Regenerative Medicine
- Amino Acid Enzymes and Metabolism
- Genetic Neurodegenerative Diseases
- Glioma Diagnosis and Treatment
- Parkinson's Disease Mechanisms and Treatments
- Nanoparticle-Based Drug Delivery
- Cancer Research and Treatments
- RNA Research and Splicing
- Microtubule and mitosis dynamics
- 3D Printing in Biomedical Research
- Advanced biosensing and bioanalysis techniques
- Histone Deacetylase Inhibitors Research
- Semiconductor materials and devices
- Cancer therapeutics and mechanisms
- Molecular Biology Techniques and Applications
- Neurological and metabolic disorders
- Pain Mechanisms and Treatments
- Genomics, phytochemicals, and oxidative stress
- Cancer Cells and Metastasis
- Genetics and Neurodevelopmental Disorders
- Bone Tissue Engineering Materials
Columbia University
2019-2023
New York Hospital Queens
2019-2023
NewYork–Presbyterian Hospital
2019-2023
Columbia University Irving Medical Center
2019-2023
University of Florida
2014-2016
Allen Institute for Brain Science
2014-2016
Florida College
2014-2016
University at Buffalo, State University of New York
2012
Dysregulated energetics coupled with uncontrolled proliferation has become a hallmark of cancer, leading to increased interest in metabolic therapies. Glioblastoma (GB) is highly malignant, very metabolically active, and typically resistant current Dietary treatment options based on glucose deprivation have been explored using restrictive ketogenic diet (KD), positive anticancer reports. However, negative side effects lack palatability make the KD difficult implement an adult population....
Abstract Tumor-initiating cells (TIC) perpetuate tumor growth, enable therapeutic resistance, and drive initiation of successive tumors. Virtually nothing is known about the role mechanotransductive signaling in controlling TIC tumorigenesis, despite recognized importance altered mechanics tissue dysplasia common observation that extracellular matrix (ECM) stiffness strongly regulates cell behavior. To address this open question, we cultured primary human glioblastoma (GBM) TICs on...
A better understanding of the molecules implicated in growth and survival glioblastoma (GBM) cells their response to temozolomide (TMZ), standard-of-care chemotherapeutic agent, is necessary for development new therapies that would improve outcome current GBM treatments. In this study, we characterize role pericentriolar material 1 (PCM1), a component centriolar satellites surrounding centrosomes, cell proliferation sensitivity genotoxic agents such as TMZ. We show PCM1 expressed around...
Neurodegeneration is a protracted process involving progressive changes in myriad cell types that ultimately results the death of vulnerable neuronal populations. To dissect how individual within heterogeneous tissue contribute to pathogenesis and progression neurodegenerative disorder, we performed longitudinal single-nucleus RNA sequencing mouse human spinocerebellar ataxia type 1 (SCA1) cerebellar tissue, establishing continuous dynamic trajectories each population. Importantly, defined...
Summary Increasing only the expression of pleiotropic cytokine tumor necrosis factor–alpha exclusively in hippocampus naïve rats induces peripheral hypersensitivity to thermal and mechanical stimulation reminiscent chronic pain behaviors. The manifestation chronic, neuropathic includes elevated levels (TNF). Previously, we have shown that hippocampus, an area brain most notable for its role learning memory formation, plays a fundamental sensation. Using animal model pain, demonstrated...
Although the majority of MDS patients fail to achieve clinical improvement approved therapies, some benefit from treatment. Predicting patient response prior therapy would improve treatment effectiveness, avoid treatment-related adverse events and reduce healthcare costs. Three separate cohorts were used simulate drug lenalidomide alone, hypomethylating agent (HMA) or HMA plus lenalidomide. Utilizing a computational biology program, genomic abnormalities in each create an intracellular...
Laser capture microdissection (LCM) is an advantageous tool that allows for the collection of cytologically and/or phenotypically relevant cells or regions from heterogenous tissues. Captured product can be used in a variety molecular methods protein, DNA RNA isolation. However, preservation postmortem human brain tissue especially challenging. Standard visualization techniques LCM require histologic immunohistochemical staining procedures further degrade RNA. Therefore, we designed...
Glioblastoma [GB] is the deadliest and most malignant type of brain tumor, with a median survival less than 12 months. Research into tumor metabolism Warburg Effect have shed new light on an idea that puts energy disparities at forefront for innovative treatment options patients. Tumor cells established dependence glucose plays significant role in growth survival. The human body retains secondary sources can be utilized times starvation deprivation. By exchanging ketones (β-hydroxybutyrate)...
Abstract Topoisomerase II poisons are one of the most common class chemotherapeutics used in cancer. We show that glioblastoma (GBM), malignant all primary brain tumors adults is responsive to TOP2 poisons. To identify genes confer susceptibility this drug gliomas, we performed a genome-scale CRISPR knockout screen with etoposide. Genes involved protein synthesis and DNA damage were implicated etoposide susceptibility. define potential biomarkers for poisons, hits overlapped whose expression...
Laser capture microdissection (LCM) is an advantageous tool that allows for the collection of cytologically and/or phenotypically relevant cells or regions from heterogenous tissues. Captured product can be used in a variety molecular methods protein, DNA RNA isolation. However, preservation postmortem human brain tissue especially challenging. Standard visualization techniques LCM require histologic immunohistochemical staining procedures further degrade RNA. Therefore, we designed...
Application of toxic compounds used at maximum tolerated dose until resistance develops is the most commonly experienced outcome in cancer therapy. Based on successful concepts borrowed from field ecology to manage pest populations, we developed a therapeutic strategy relying use multiple independent and non-toxic stressors that enable efficient horizontal vertical targeting tumor. Our treatment (CA.001) uses combination two distinct approaches. The first aspect our approach involves...
INTRODUCTION: Tumorigenesis and resistance to treatment result from the ability of specific subpopulations tumor cells expand survive. Conventional therapies effectively eliminate rapidly dividing cells, which derive their energy aerobic glycolysis, but spare slowly populations. We previously reported existence slow-cycling that exhibit cancer stem cell (CSC) characteristics in glioblastoma (GB). Clinical strategies targeting this phenotype could lead improved therapeutic outcomes reduce...
Abstract Background: Despite three FDA approved standard of care (SOC) therapeutics, 60% patients with myelodysplastic syndromes (MDS) fail to achieve hematologic improvement and nearly all die refractory disease. The ability predict patient responses SOC therapy would improve treatment efficacy, avoid treatment-related adverse events reduce health costs. Ideally, a patient's prediction be based on their cancer mutanome. Methods: A prospective clinical trial was initiated in June 2015 (1)...
A major obstacle in the treatment of glioblastoma (GBM) is blood brain barrier (BBB), which restricts drug molecules bloodstream from reaching tumor. The BBB may be disrupted at or near GBM tumor core, but it intact growing edge tumor, where invasive cells reside and can cause disease recurrence. To combat more effectively, novel transport technology that crosses urgently needed. Brain microvascular endothelial (BMEC) found are lined with low-density lipoprotein receptors (LDLr) LDL-like...
<div>Abstract<p>Tumor-initiating cells (TIC) perpetuate tumor growth, enable therapeutic resistance, and drive initiation of successive tumors. Virtually nothing is known about the role mechanotransductive signaling in controlling TIC tumorigenesis, despite recognized importance altered mechanics tissue dysplasia common observation that extracellular matrix (ECM) stiffness strongly regulates cell behavior. To address this open question, we cultured primary human glioblastoma...