A5017270940- Hearing, Cochlea, Tinnitus, Genetics
- Marine animal studies overview
- Hearing Loss and Rehabilitation
- Developmental Biology and Gene Regulation
- Ear Surgery and Otitis Media
- Antimicrobial Peptides and Activities
- Animal Vocal Communication and Behavior
- Congenital heart defects research
- Immune Response and Inflammation
- Anatomy and Medical Technology
- Cancer-related molecular mechanisms research
- Acoustic Wave Phenomena Research
- melanin and skin pigmentation
- Cancer Cells and Metastasis
- Noise Effects and Management
- RNA and protein synthesis mechanisms
- Bat Biology and Ecology Studies
- Biochemical Analysis and Sensing Techniques
- Surgical Simulation and Training
- Neurogenesis and neuroplasticity mechanisms
- Connexins and lens biology
- Cell Adhesion Molecules Research
- Ion Channels and Receptors
- Vestibular and auditory disorders
- Underwater Vehicles and Communication Systems
University of Illinois Chicago
2017-2018
University of Massachusetts Chan Medical School
2016
Harvard University
2004-2015
Harvard–MIT Division of Health Sciences and Technology
2005-2013
Boston Children's Hospital
1999-2013
Vertex Pharmaceuticals (United States)
2011
Boston University
1992-2010
Institute of Cell Biology and Neurobiology
2009
Boston Medical Center
2008
University Medical Center
2008
Any loss of cochlear hair cells has been presumed to result in a permanent hearing deficit because the production these normally ceases before birth. However, after acoustic trauma, injured sensory mature cochlea chicken are replaced. New appear be produced by mitosis supporting that survive at lesion site and do not divide absence trauma. This trauma-induced division postmitotic may lead recovery from profound loss.
Abstract The avian auditory epithelium is capable of complete regeneration after hair cell (HC) loss. Most new HCs arise via division, but approximately one‐third direct transdifferentiation (DT), in which supporting cells (SCs) alter their phenotype without dividing. In this study, we used synchronous, gentamicin‐induced near‐total HC loss the basal end and continuous infusion division marker bromodeoxyuridine (BrdU) to identify origin each individual regenerating HC. Early were identified...
Abstract Birds respond to hair cell loss by stimulating division in the otherwise mitotically quiescent sensory epithelium and generating new cells. We examined proliferation during regeneration chick basilar papilla using 5‐bromo‐2′ ‐deoxyuridine (BrdU). Chicks were noise exposed for 4 or 24 hours injected with BrdU, cochleae immunohistochemically labeled detect BrdU. Immunoreactivity after short‐term postinjection survival identified when cells entered S phase. For both hour exposures,...
To determine the spectrum of connexin 26 (Cx26) mutations and their phenotypes in children with sensorineural hearing loss (SNHL) or mixed (MHL).Children SNHL MHL were prospectively tested for entire coding region Cx26 gene.Children no obvious etiology loss.Between December 1, 1998, July 2000, 107 patients from 99 families underwent testing. Most aged 1 week to 16 years (61 boys 46 girls). Thirty (30%) probands had mutations: biallelic detected 18 (9 homozygous 9 compound heterozygous)...
Abstract Aminoglycoside treatment induces caspase‐dependent apoptotic death in inner ear sensory hair cells. The timing of signaling cells following systemic aminoglycoside has not been characterized vivo. We administered a single subcutaneous injection the gentamicin (300 mg/kg) to 12–16‐day‐old chicks and used immunocytochemical techniques document responses affected cells: T‐cell restricted intracellular antigen‐related protein (TIAR) translocation from nucleus cytoplasm, cytochrome c...
The sensory epithelium of the chick cochlea contains only two cell types, hair cells and supporting cells. We developed methods to rapidly dissect out prepare a detergent-extracted cytoskeleton. High salt treatment cytoskeleton leaves "hair border", containing actin filament bundles stereocilia still attached cuticular plate. On SDS-PAGE stained with silver intact is seen contain large number bands, most prominent which are calbindin actin. Detergent extraction solubilizes proteins including...
Department of Anatomy & Neurobiology; Boston University School Medicine; Boston, Massachusetts 02118
Abstract Oxidative injury to premyelinating oligodendrocytes (preOLs) in developing white matter has been implicated the pathogenesis of periventricular leukomalacia, lesion underlying most cases cerebral palsy premature infants. In this study, we investigated pathways OL death induced by intracellular glutathione (GSH) depletion. We found that lipoxygenase (LOX) inhibitors AA‐861 and BMD‐122 (N‐benzyl‐N‐hydroxy‐5‐phenylpentamide; BHPP), but not cyclooxygenase (COX) inhibitor indomethacin,...
The sensory hair cells of the inner ear undergo apoptosis after acoustic trauma or aminoglycoside antibiotic treatment, causing permanent auditory and vestibular deficits in humans. Previous studies have demonstrated a role for caspase activation cell death ototoxic injury that can be reduced by concurrent treatment with inhibitors vitro. In this study, we examined protective effects inhibition on vivo systemic injections aminoglycosides. one series experiments, chickens were implanted...
ABSTRACT Beginning in 8-day embryos, stereocilia sprout from the apical surface of hair cells apparently at random. As embryo continues to develop, number increases. By 10 1/2 days is approximately same as that encountered extending mature relative positions adult cochlea. Surprisingly, over next 2–3 increase so a 12-day have 1 2 times many cells. In short, there an overshoot stereociliary number. During period which are formed (9–12 days) each cell filled with closely packed stereocilia;...
Abstract The developmental mechanisms that allow physiological coding of acoustic pitch have remained unexplained. Cochlear hair cells different structures respond to sound frequencies and synapse with neurons project locations in the brain. How do these develop appropriate structures, how are connections between specific code for their sensitivities matched? We investigated one aspect this by denervating embryonic chicken ears, before time cell production, then transplanting them aneural...