A5033534879- Cellular transport and secretion
- Neuroscience and Neuropharmacology Research
- Lipid Membrane Structure and Behavior
- Autophagy in Disease and Therapy
- Force Microscopy Techniques and Applications
- Neurobiology and Insect Physiology Research
- Advanced Electron Microscopy Techniques and Applications
- Enzyme Structure and Function
- Advanced Fluorescence Microscopy Techniques
- Neurogenesis and neuroplasticity mechanisms
- Fermentation and Sensory Analysis
- Effects of Environmental Stressors on Livestock
- Neuroinflammation and Neurodegeneration Mechanisms
- Cell Image Analysis Techniques
- Meat and Animal Product Quality
- Horticultural and Viticultural Research
University of Göttingen
2022-2024
Universitätsmedizin Göttingen
2022-2024
Max Delbrück Center
2021
Freie Universität Berlin
1972-2020
Abstract Fluorescence imaging is one of the most versatile and widely-used tools in biology 1 . Although techniques to overcome diffraction barrier were introduced more than two decades ago, nominal attainable resolution kept improving 2, 3 , fluorescence microscopy still fails image morphology single proteins or small molecular complexes, either purified a cellular context 4, 5 Here we report solution this problem, form o ne-step n anoscale e xpansion (ONE) microscopy. We combined 10-fold...
The attainable resolution of fluorescence microscopy has reached the subnanometer range, but this technique still fails to image morphology single proteins or small molecular complexes. Here, we expand specimens at least tenfold, label them with conventional fluorophores and light microscopes, acquiring videos in which analyze fluctuations. One-step nanoscale expansion (ONE) enables visualization shapes individual membrane soluble proteins, achieving around 1-nm resolution. We show that...
Microglia, the resident macrophages in central nervous system, express receptors for classical neurotransmitters, such as γ-aminobutyric acid (GABA) and glutamate, suggesting that they sense synaptic activity. To detect microglial Ca2+ responses to neuronal activity, we generate transgenic mouse lines expressing fluorescent indicator GCaMP6m, specifically microglia demonstrate electrical stimulation of Schaffer collateral pathway results early postnatal but not adult hippocampus. Preceding...
Abstract Brain wiring is remarkably precise, yet most neurons readily form synapses with incorrect partners when given the opportunity. Dynamic axon-dendritic positioning can restrict synaptogenic encounters, but spatiotemporal interaction kinetics and their regulation remain essentially unknown inside developing brains. Here we show that of axonal filopodia synapse formation partner choice for are not otherwise prevented from making synapses. Using 4D imaging in Drosophila brains,...
Synaptic transmission is driven by a complex cycle of vesicle docking, release, and recycling, maintained distinct pools. However, the partitioning pools reserve pool recruitment remain poorly understood. We use novel modeling technology to model synaptic in unprecedented molecular spatial detail at hippocampal synapse. Our demonstrates robust recycling vesicles that maintains consistent even during sustained high-frequency firing. also show how cytosolic proteins synapsin-1 tomosyn-1...
Neuronal communication relies on synaptic vesicle recycling, which has long been investigated by live imaging approaches. Synapto-pHluorins, genetically encoded reporters that incorporate a pH-sensitive variant of GFP within the lumen vesicle, have especially popular. However, they require genetic manipulation, implying tool combining their excellent reporter properties with ease use classical immunolabeling would be desirable. We introduce this here, relying primary antibodies against...
Abstract The synaptic vesicle cluster (SVC) is an essential component of chemical synapses, which provides neurotransmitter‐loaded vesicles during activity, at the same time as also controlling local concentrations numerous exo‐ and endocytosis cofactors. In addition, SVC hosts molecules that participate in other aspects function, from cytoskeletal components to adhesion proteins, affects location function organelles such mitochondria endoplasmic reticulum. We argue here these features...
Summary Brain wiring is remarkably precise, yet most neurons readily form synapses with incorrect partners when given the opportunity. Dynamic axon-dendritic positioning can restrict synaptogenic encounters, but spatiotemporal interaction kinetics and their regulation remain essentially unknown inside developing brains. Here we show that of axonal filopodia synapse formation partner choice for are not otherwise prevented from making synapses. Using 4D imaging in Drosophila brains, filopodial...