A5052819846- Cellular transport and secretion
- Lipid Membrane Structure and Behavior
- Advanced Fluorescence Microscopy Techniques
- RNA Research and Splicing
- Microtubule and mitosis dynamics
- Neuroscience and Neuropharmacology Research
- Skin and Cellular Biology Research
- Protein Structure and Dynamics
- Retinal Development and Disorders
- Parkinson's Disease Mechanisms and Treatments
- Supramolecular Self-Assembly in Materials
- Cellular Mechanics and Interactions
- Cell Image Analysis Techniques
- CRISPR and Genetic Engineering
- Pluripotent Stem Cells Research
Max Planck Institute for Medical Research
2022-2025
Max Planck Institute for Multidisciplinary Sciences
2022-2025
Abstract Neurons grow neurites of several tens micrometers in length, necessitating active transport from the cell body by motor proteins. By tracking fluorophores as minimally invasive labels, MINFLUX is able to quantify motion those proteins with nanometer/millisecond resolution. Here we study substeps a truncated kinesin-1 mutant primary rat hippocampal neurons, which have so far been mainly observed on polymerized microtubules deposited onto glass coverslips. A gentle fixation protocol...
Neurofilaments are one of the main cytoskeletal components in neurons; they can be found form oligomers at pre- and postsynapses. How their presence is regulated postsynapse remains largely unclear. Here we systematically quantified, by immunolabeling, occurrence neurofilament isoform triplet light (NFL), medium (NFM), heavy (NFH) using STED nanoscopy together with markers synaptic strength activity. Our data show that, within dendritic spines, isoforms rarely colocalize each other that...
Abstract The postsynaptic density component PSD-95 undergoes activity-dependent plasticity mechanisms that rely on protein synthesis and structural remodeling. How synaptic activity can influence these dynamics at the single synapse level remains unclear. Here we combine genome-editing, pulse-chase experiments, STED 3D MINFLUX nanoscopy hippocampal neuronal cultures to study integration of newly translated molecules sites their rearrangement within individual clusters near-molecular...
The submembrane cytoskeleton of neurons displays a highly ordered 190-nanometer periodic actin-spectrin lattice, the membrane-associated skeleton (MPS). It is involved in mechanical resilience, signaling, and action potential transmission. Here, we identify paralemmin-1 (Palm1) as component regulator MPS. Palm1 binds to amino-terminal region βII-spectrin, MINFLUX microscopy localizes it close proximity (<20 nanometers) actin-capping protein MPS adducin. Combining overexpression, knockout,...
Summary The Membrane-associated Periodic Skeleton (MPS) is a specialized submembrane cytoskeleton of neuronal cells, characterized by highly ordered 190 nm periodic lattice, with emerging functions in mechanical resilience, inter- and intracellular signaling, action potential transmission. Here, we identify Paralemmin-1 (Palm1) as new component regulator the MPS. Palm1 binds to N-terminal region βII-spectrin, core MPS component, periodically organized along axon hippocampal neurons. Applying...
Abstract The synaptic vesicle cluster (SVC) is an essential component of chemical synapses, which provides neurotransmitter‐loaded vesicles during activity, at the same time as also controlling local concentrations numerous exo‐ and endocytosis cofactors. In addition, SVC hosts molecules that participate in other aspects function, from cytoskeletal components to adhesion proteins, affects location function organelles such mitochondria endoplasmic reticulum. We argue here these features...
Neurons grow neurites of several tens micrometers in length, necessitating active transport from the cell body by motor proteins. By tracking fluorophores as minimally invasive labels, MINFLUX is able to quantify motion those proteins with nanometer/millisecond resolution. Here we study substeps a truncated kinesin-1 mutant primary rat hippocampal neurons, which have so far been mainly observed on microtubules polymerized glass coverslips. A gentle fixation protocol largely maintains...
Abstract Neurofilaments are one of the main cytoskeletal components in neurons and they can be found form oligomers at pre- postsynapses. How their presence is regulated postsynapse remains widely unclear. Here we systematically quantified by immunolabeling occurrence neurofilament isoform triplet light (NFL), medium (NFM), heavy (NFH) with STED nanoscopy together markers synaptic strength activity. Our data shows that within dendritic spines isoforms rarely colocalize each other present to...