Abstract Background Recent data indicated that macrophages may mutually interact with cancer cells to promote tumor progression and chemoresistance, but the interaction in cholangiocarcinoma (CCA) is obscure. Methods 10x Genomics single-cell sequencing technology was used identified role of CCA. Then, we measured expression prognostic macrophage markers aPKC ɩ 70 human CCA tissues. Moreover, constructed monocyte-derived (MDMs) generated from peripheral blood monocytes (PBMCs) polarized them...

Autophagy is an important bioprocess throughout the occurrence and development of cancer. However, role autophagy-related lncRNAs in pancreatic cancer (PC) remains obscure. In study, we identified (ARlncRNAs) divided PC patients from The Cancer Genome Atlas into training validation set. Firstly, constructed a signature set by least absolute shrinkage selection operator penalized cox regression analysis multivariate analysis. Then, validated independent prognostic risk both with survival...

Abstract Background The intricate landscape of immunocytes in the tumor microenvironment (TME) is fundamental to immunotherapy but notably under-researched extrahepatic cholangiocarcinoma (ECCA). Methods Single-cell RNA sequencing technology was conducted make an in-depth analysis from matched tissues, paratumor tissues and peripheral blood ECCA patients. potential cellular interactions between two cell populations were analyzed with software CellPhoneDB (v2.1.7). Results We obtained 13526...

As a new type of post-translational modification (PTM), lysine 2-hydroxyisobutyrylation (K hib ) was firstly identified in histones and functioned as regulator transactivation mammals. However, the role K proteins remains to be investigated. Here, we 10,367 sites on 2,325 modified seven patients with pancreatic cancer by applying liquid chromatography tandem mass spectrometry (LC-MS/MS) qualitative proteomics techniques. Among them, 27 -modified were histones. Bioinformatics analysis...

Abstract Gallbladder cancer (GBC) is a highly malignant bile duct with poor prognosis due to early invasion and metastasis. However, the molecular mechanisms through which GBC cells interact tumor microenvironment (TME) remain poorly understood. Here, we examined role of suppressor apoptosis-stimulating p53 protein 2 (ASPP2) in regulating metastasis macrophage recruitment. The clinicopathological significance ASPP2 expression was measured by immunohistochemical analysis 72 patients GBC....

Gallbladder cancer (GBC) is a highly malignant bile duct with poor prognosis characterized by its insensitivity to chemotherapy. Emerging evidence indicates that cytoprotective antioxidation involved in drug resistance of various cancers; however, the underlying molecular mechanisms remain obscure. Here, we demonstrated atypical protein kinase Cι (aPKCι) mediated reactive oxygen species (ROS) inhibition kinase-independent manner, which played crucial role tumorigenesis and chemoresistance....

Background: Globally, pancreatic adenocarcinoma (PAAD) is a common and highly devastating gastrointestinal malignancy that seriously threatens human health. Pyroptosis refers to an emerging form of programmed cell death has been discovered in recent years, studies have demonstrated long non-coding RNA (lncRNA) may act as moderator the pyroptosis process cancer cells. However, relevant explorations about lncRNAs are still insufficient PAAD. Therefore, our research designed make comprehensive...

Cholangiocarcinoma (CCA) is a lethal malignancy in the hepatobiliary system, with dysregulated protein expression and phosphorylation signaling. However, signatures of CCAs are little-known. Here, we performed proteomic phosphoproteomic profiling tumors normal adjacent tissues (NATs) from patients CCA predicted eleven PKs high-potentially related to comprehensive inference functional kinases (PKs) (CifPK) pipeline. Besides two known CCA-associated PKs, screened remaining candidates uncovered...

Background Despite advancements in hepatocellular carcinoma (HCC) treatments, the prognosis for patients remains suboptimal. Cumulative evidence suggests that programmed cell death (PCD) exerts crucial functions HCC. PCD-related genes are potential predictors and therapeutic responses. Methods A systematic analysis of 14 PCD modes was conducted to determine correlation between novel machine learning-based integrative framework utilized construct Index (PCDI) response prediction....

<title>Abstract</title> <bold>Purpose: </bold>This study used a two-sample Mendelian randomization (MR) method to explore the potential causal relationship between air quality,<bold> </bold>cholelithiasis and its related risk factor, blood lipids. <bold>Patients methods:</bold> This in European East Asian groups, utilizing publicly available GWAS data. The main was inverse variance weighting (IVW), with additional quality checks including weighted median, MR-Egger, simple, models. Cochran's...

SUMOylation plays a crucial role in numerous cellular biological and pathophysiological processes associated with human disease; however, the mechanisms regulating genes involved remain unclear. In present study, E2F transcription factor 4 (E2F4) was identified as an member related to hepatocellular carcinoma (HCC) progression by public database analysis. It found that E2F4 promoted proliferation invasiveness of HCC cells via using Soft agar Transwell migration assays. Mechanistically, it...

Background: Cuproptosis and necroptosis represent two distinct programmed cell death modalities implicated in neoplastic progression; however, the role of combining cuproptosis hepatocellular carcinoma (HCC) remains to be elucidated. Methods: A total 29 cuproptosis-related genes (CRNGs) were identified, followed by an extensive analysis their mutational characteristics, expression patterns, prognostic implications, associations with tumor microenvironment (TME). Subsequently, a CRNG...

Abstract Objective Hepatocellular carcinoma (HCC) is an extremely deadly cancer with few effective therapeutic options available. Ceramide synthases (CERS), a family of enzymes that regulate sphingolipid metabolism, have been suggested to play role in initiation and progression. Whereas the specific functions CERS HCC pathogenesis not yet fully elucidated. Methods The TCGA ICGC databases were employed analyze expression levels clinical relevance genes HCC. Functional enrichment analyses...

Abstract Objective Hepatocellular carcinoma (HCC) is an extremely deadly cancer with few effective therapeutic options available. Ceramide synthases (CERS), a family of enzymes that regulate sphingolipid metabolism, have been suggested to play role in initiation and progression. Whereas the specific functions CERS HCC pathogenesis not yet fully elucidated. Methods The TCGA ICGC databases were employed analyze expression levels clinical relevance genes HCC. Functional enrichment analyses...

Abstract Background Hepatocellular carcinoma (HCC) is the second major cause of tumor death worldwide, threatening people's lives and health. Circulating cells (CTCs) facilitate progression many cancers, including HCC. Methods Combining transcriptomic data from TCGA, ICGC ctcRbase databases, we characterized differentially expressed circulating cells/microemboli (CTCs/CTMs) related genes (CRGs). These CRGs were then analyzed with a lasso multivariate COX, five-gene prognostic signature was...