A5090399718- Viral Infections and Immunology Research
- Animal Virus Infections Studies
- Viral gastroenteritis research and epidemiology
- Animal Disease Management and Epidemiology
- Virus-based gene therapy research
- Advanced biosensing and bioanalysis techniques
- Herpesvirus Infections and Treatments
- Monoclonal and Polyclonal Antibodies Research
- Vector-Borne Animal Diseases
- SARS-CoV-2 detection and testing
- Biosensors and Analytical Detection
Lanzhou Veterinary Research Institute
2014-2025
Chinese Academy of Agricultural Sciences
2014-2025
Lanzhou University
2023-2025
Ningxia University
2025
Yangtze University
2022
Bovine Coronavirus (BCoV) is a significant pathogen responsible for neonatal calf diarrhea, winter dysentery in adult cattle, and bovine respiratory diseases. Infection with the virus can result hemorrhagic decreased milk production, potentially fatal outcomes leading to considerable economic repercussions cattle industry. Efficient management of BCoV relies on swift precise detection techniques. CHO cells were utilized express secreted recombinant nucleocapsid protein (N), whereby rabbit...
ABSTRACT Porcine deltacoronavirus (PDCoV) is a significant emerging pathogen that causes severe enteric disease in swine, and therefore economic losses the pig farming industry. Here, we developed novel double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) based on two monoclonal antibodies directed against PDCoV spike protein. These were obtained through hybridoma fusion screening, they can specifically react with The detection limits of DAS-ELISA for recombinant protein...
Classical swine fever (CSF) is a severe infectious disease of pigs that causes significant economic losses to the industry.This study developed solid-phase blocking enzyme-linked immunosorbent assay (spbELISA) method for specific detection antibodies against CSF virus (CSFV) in porcine serum samples.A spbELISA was based on recombinant E2 expressed Escherichia coli. The specificity this established evaluated using reference samples positive other common diseases. stability and sensitivity...
Viroporins are a group of transmembrane proteins with low molecular weight that encoded by many animal viruses. Generally, viroporins composed 50-120 amino acid residues and possess minimum one hydrophobic region interacts the lipid bilayer leads to dispersion. involved in destroying morphology host cells disturbing their biological functions complete life cycle virus. The 2B enteroviruses, which belong family Picornaviridae, can form pores oligomerization, increase permeability plasma...