A5024926939- CRISPR and Genetic Engineering
- Antibiotic Resistance in Bacteria
- Vibrio bacteria research studies
- Bacterial Genetics and Biotechnology
- Vector-borne infectious diseases
- Vector-Borne Animal Diseases
- RNA and protein synthesis mechanisms
- Insect and Pesticide Research
- Gut microbiota and health
- Evolution and Genetic Dynamics
- Animal Disease Management and Epidemiology
- Bacteriophages and microbial interactions
University of Wisconsin–Madison
2020-2024
Bates College
2020
Acinetobacter baumannii is a hospital-acquired pathogen that resistant to many common antibiotic treatments. To combat A. infections, we need identify promising therapeutic targets and effective combinations. In this study, comprehensively characterize the genes pathways are critical for viability. We show involved in aerobic metabolism central physiology may represent appealing drug targets. also find antibiotic-gene interactions impact efficacy of carbapenems, rifamycins, polymyxins,...
Abstract Targeted, genome-scale gene perturbation screens using Clustered Regularly Interspaced Short Palindromic Repeats interference (CRISPRi) and activation (CRISPRa) have revolutionized eukaryotic genetics, advancing medical, industrial, basic research. Although CRISPRi knockdowns been broadly applied in bacteria, options for overexpression face key limitations. Here, we develop a facile approach bacteria call, “CRISPRtOE” (CRISPR transposition OverExpression). We create platform...
Abstract Facile bacterial genome sequencing has unlocked a veritable treasure trove of novel genes awaiting functional exploration. To make the most this opportunity requires powerful genetic tools that can target all in diverse bacteria. CRISPR interference (CRISPRi) is programmable gene‐knockdown tool uses an RNA‐protein complex comprised single guide RNA (sgRNA) and catalytically inactive Cas9 nuclease (dCas9) to sterically block transcription genes. We previously developed suite modular...
Abstract The emergence of multidrug-resistant Gram-negative bacteria underscores the need to define genetic vulnerabilities that can be therapeutically exploited. pathogen, Acinetobacter baumannii , is considered an urgent threat due its propensity evade antibiotic treatments. Essential cellular processes are target existing antibiotics and a likely source new vulnerabilities. Although A. essential genes have been identified by transposon sequencing (Tn-seq), they not prioritized sensitivity...
Gene expression systems that transcend species barriers are needed for cross-species analysis of gene function. In particular, can be utilized in both model and pathogenic bacteria underpin comparative functional approaches inform conserved variable features bacterial physiology. Here, we develop replicative integrative vectors alongside a novel, IPTG-inducible promoter used the bacterium
Abstract The Gram-negative pathogen Acinetobacter baumannii is considered an “urgent threat” to human health due its propensity become antibiotic resistant. Understanding the distinct regulatory paradigms used by A. mitigate cellular stresses may uncover new therapeutic targets. Many γ-proteobacteria use extracytoplasmic function (ECF) σ factor, RpoE, invoke envelope homeostasis networks in response stress. species contain poorly characterized ECF “SigAb;” however, it unclear if SigAb has...
ABSTRACT Gene expression systems that transcend species barriers are needed for cross-species analysis of gene function. In particular, can be utilized in both model and pathogenic bacteria underpin comparative functional approaches inform conserved variable features bacterial physiology. this study, we develop replicative integrative vectors alongside a novel, IPTG-inducible promoter used the bacterium Escherichia coli K-12 as well strains antibiotic-resistant pathogen, Acinetobacter...
Abstract The emergence of multidrug-resistant Gram-negative bacteria underscores the need to define genetic vulnerabilities in relevant pathogens. pathogen, Acinetobacter baumannii , poses an urgent threat by evading antibiotic treatment through both intrinsic and acquired mechanisms. Antibiotics kill targeting essential gene products, but antibiotic-essential interactions have not been studied systematically A. . Here, we use CRISPR interference (CRISPRi) comprehensively phenotype genes. We...