A5020541911- Cholesterol and Lipid Metabolism
- Drug Transport and Resistance Mechanisms
- Lipid Membrane Structure and Behavior
- Lipid metabolism and biosynthesis
- Peroxisome Proliferator-Activated Receptors
- Pharmacological Effects and Toxicity Studies
- Adenosine and Purinergic Signaling
- Trace Elements in Health
- Machine Learning in Bioinformatics
- Liver Disease Diagnosis and Treatment
- HIV/AIDS drug development and treatment
- Fatty Acid Research and Health
- Diet, Metabolism, and Disease
- Advanced biosensing and bioanalysis techniques
- Pediatric Hepatobiliary Diseases and Treatments
- Receptor Mechanisms and Signaling
- RNA and protein synthesis mechanisms
Kyoto University
2018-2025
Material Sciences (United States)
2019
Abstract The quality and quantity of high-density lipoprotein (HDL) in blood plasma are important for preventing coronary artery disease. ATP-binding cassette protein A1 (ABCA1) apolipoprotein A-I (apoA-I) play essential roles nascent HDL formation, but controversy persists regarding the mechanism by which is generated. In “direct loading model”, apoA-I acquires lipids directly from ABCA1 while it bound to transporter. By contrast, “indirect specific membrane domains created ABCA1. this...
Intracellular cholesterol transport is essential for maintaining cellular homeostasis. ABCA1 continuously moves from the inner leaflet to outer of plasma membrane (PM) maintain low levels. When PM levels exceed ER levels, which are maintained at approximately 5 mol% by complex sterol regulatory element-binding protein (SREBP) and SREBP cleavage-activating (SCAP), Aster-A/GramD1a transports excess ER. Furthermore, removes promoting its efflux as nascent high-density lipoprotein (HDL)...
ATP-binding cassette A1 (ABCA1) plays a key role in generating high-density lipoprotein (HDL) and preventing atherosclerosis. ABCA1 exports cholesterol phospholipid to apolipoprotein A-I (apoA-I) serum generate HDL. We found that streptolysin O (SLO), cholesterol-dependent pore-forming toxin, barely formed pores ABCA1-expressing cells, even the absence of apoA-I. Neither content cell membranes nor amount SLO bound cells was affected by ABCA1. On other hand, binding D4 domain perfringolysin...
ATP-Binding Cassette A1 (ABCA1) is a key lipid transporter for cholesterol homeostasis. We recently reported that ABCA1 not only exports excess in an apoA-I dependent manner, but it also flops from the inner to outer leaflet of plasma membrane. However, relationship between these two activities still unclear. In this study, we analyzed subcellular localization by using newly generated monoclonal antibody against its extracellular domain and functions eleven chimera proteins, which C-terminal...
P-glycoprotein (P-gp; also known as MDR1 or ABCB1) is an ATP-driven multidrug transporter that extrudes various hydrophobic toxic compounds to the extracellular space. P-gp consists of two transmembrane domains (TMDs) form substrate translocation pathway and nucleotide-binding (NBDs) bind hydrolyze ATP. At least states are required for transport. In inward-facing (pre-drug transport) conformation, NBDs separated, TMDs open intracellular side; in outward-facing (post-drug dimerized, slightly...
Abstract P-glycoprotein (P-gp; also known as MDR1 or ABCB1) is an ATP-driven multidrug transporter that extrudes various hydrophobic toxic compounds to the extracellular space. P-gp consists of two transmembrane domains (TMDs) form substrate translocation pathway and nucleotide-binding (NBDs) bind hydrolyze ATP. takes at least states during transport; inward-facing (pre-drug transport) conformation, in which NBDs are separated TMDs open intracellular side, outward-facing (post-drug dimerized...
Abstract Cholesterol is a major and essential component of the mammalian cell plasma membrane (PM) loss cholesterol homeostasis leads to various pathologies. Cellular uptake synthesis are regulated by sensor in endoplasmic reticulum (ER). However, it remains unclear how PM level sensed. Here we show that sensing depends on ATP-binding cassette A1 (ABCA1) Aster-A, which cooperatively maintain asymmetric transbilayer distribution PM. ABCA1 translocates (flops) from inner outer leaflet low...