A5100615202- Monoclonal and Polyclonal Antibodies Research
- Protein purification and stability
- Glycosylation and Glycoproteins Research
- Viral Infectious Diseases and Gene Expression in Insects
- Galectins and Cancer Biology
- SARS-CoV-2 and COVID-19 Research
- RNA Interference and Gene Delivery
- Antifungal resistance and susceptibility
- Fungal Infections and Studies
- Bacteriophages and microbial interactions
- Heat shock proteins research
- Bee Products Chemical Analysis
- Postharvest Quality and Shelf Life Management
- MicroRNA in disease regulation
- Peripheral Nerve Disorders
- Biosensors and Analytical Detection
- Plant Disease Management Techniques
- Studies on Chitinases and Chitosanases
- vaccines and immunoinformatics approaches
- Cancer Treatment and Pharmacology
- Fibroblast Growth Factor Research
- Phytochemicals and Antioxidant Activities
- Extracellular vesicles in disease
- Growth Hormone and Insulin-like Growth Factors
- Powdery Mildew Fungal Diseases
National Research Council Canada
2012-2023
Osong Medical Innovation Foundation
2018-2021
Rural Development Administration
2020
Stanford Medicine
2018
University of Ulsan
1999-2015
Asan Medical Center
1999-2015
Ulsan College
2014-2015
National Academies of Sciences, Engineering, and Medicine
2010-2014
Institute for Biological Sciences
2012
Catholic University of Korea
2006
The C-type lectin-like domain of CLEC14a (CLEC14a-C-type [CTLD]) is a key that mediates endothelial cell-cell contacts in angiogenesis. However, the role CLEC14a-CTLD pathological angiogenesis has not yet been clearly elucidated. In this study, through complementarity-determining region grafting, consecutive deglycosylation, and functional isolation, we generated novel anti-angiogenic human monoclonal antibody specifically targets shows improved stability homogeneity relative to parental...
Heat shock protein 70 (Hsp70) release and its effects on pro-inflammatory cytokine production have been controversial. In this study, we investigated whether Hsp70 could be released from monocytes activates matrix metalloproteinase-9 (MMP-9) gene expression. overexpression in human monocytic cell line U937 was found to increase PMA- induced MMP-9 expression enhance motility. cDNA transfectants into culture supernatants, a part of subsequently bound the surface cells. Addition medium...
We set out to gain deeper insight into the potential of antibody light chain variable domains (VLs) as immunotherapeutics. To this end, we generated a naïve human VL phage display library and, by using method previously shown select for non-aggregating heavy (VHs), isolated diversity panning against B cell super-antigen protein L. Eight representing different germline origins were be at concentrations high 450 µM, indicating repertoires are rich source domains. In addition, VLs demonstrated...
Micafungin, a clinically important echinocandin antifungal drug, needs to be investigated as empirical therapy in febrile neutropenia comparison with azole compounds. A prospective randomized study was conducted compare clinical outcomes between micafungin and intravenous itraconazole an for hematological malignancies. The drug (micafungin 100 mg or 200 IV once daily) given high fever that sustained despite the administration of appropriate antibiotics. Treatment success determined by...
Since its first report in the Middle East 2012, respiratory syndrome-coronavirus (MERS-CoV) has become a global concern due to high morbidity and mortality of individuals infected with virus. Although majority MERS-CoV cases have been reported Saudi Arabia, overall risk areas outside remains significant as inside Arabia. Additional pandemics are expected, thus novel tools reagents for therapy diagnosis urgently needed. Here, we used phage display develop monoclonal antibodies (mAbs) that...
Human autonomous VH/VL single-domain antibodies (sdAbs) are attractive therapeutic molecules, but often suffer from suboptimal stability, solubility and affinity for cognate antigens. Most commonly, human sdAbs have been isolated in vitro display libraries constructed via synthetic randomization of rearranged domains. Here, we describe the design characterization three novel sdAb through a process of: (i) exhaustive biophysical 20 potential library scaffolds, including assessment expression...
YKL-40, also known as chitinase-3-like 1 (CHI3L1), is a glycoprotein that expressed and secreted by various cell types, including cancers macrophages. Due to its implications for upregulation in variety of diseases, inflammatory conditions, fibrotic disorders, tumor growth, YKL-40 has been considered significant therapeutic biomarker. Here, we used phage display develop novel monoclonal antibodies (mAbs) targeting human (hYKL-40). Human synthetic antibody libraries were panned against...
Since it was first reported in Wuhan, China, 2019, the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused a pandemic outbreak resulting tremendous global threat due to its unprecedented rapid spread and an absence of prophylactic vaccine or therapeutic drugs treating virus. The receptor-binding domain (RBD) SARS-CoV-2 spike protein is key player viral entry into cells through interaction with angiotensin-converting enzyme (ACE2) receptor protein, RBD therefore been...
In fed-batch cultures of recombinant Escherichia coli BL21(DE3)[pT7-G3IL2] at high cell concentration, the post-induction specific growth rate was carefully regulated by controlled medium feed to maximize synthesis level fusion interleukin-2, G3.IL-2. A maximum concentration G3.IL-2 (11.25 g l(-1)) achieved in induced culture growing 0.056 h(-1). steep decrease expression observed rates higher than its optimal value (0.056 h(-1)). cultures, plasmid multimerization and highly dependent on...
Solubility and stability are amongst the factors contributing to therapeutic efficacy of biologics. Human antibody heavy chain variable domains, VHs, one class biologics; improving VH biophysical properties is focus significant protein engineering efforts. Here, we describe an approach which involves introduction a disulfide linkage in core improves both solubility stability. More specifically, protocols for generation engineered human VHs their characterization terms formation,...
Most immunoglobulin (Ig) domains bear only a single highly conserved canonical intradomain, inter-β-sheet disulfide linkage formed between Cys23-Cys104, and incorporation of rare noncanonical linkages at other locations can enhance Ig domain stability. Here, we exhaustively surveyed the sequence tolerance variable (V) framework regions (FRs) to linkages. Starting from destabilized VH lacking Cys23-Cys104 linkage, generated screened phage-displayed libraries engineered VHs, bearing all...
Wharton’s jelly-derived mesenchymal stem cell (WJ-MSC)-derived exosomes contain a diverse cargo and exhibit remarkable biological activity, rendering them suitable for regenerative immune-modulating functions. However, the quantity of secretion is insufficient. A large body prior work has investigated use various growth factors to enhance MSC-derived exosome production. In this study, we evaluated utilization thermostable basic fibroblast factor (TS-bFGF) with MSC culture MSCs cultured...
Abstract Incorporation of noncanonical disulfide linkages into single‐domain antibodies (sdAbs) has been shown to enhance thermostability and other properties. Here, we evaluated the effects introducing a novel linkage formed between Cys residues at IMGT positions 40 55 on melting temperatures ( T m s), reversibility thermal unfolding, solubility, antigen‐binding affinities three types sdAbs (V H H, V , L domains). The Cys40‐Cys55 was tolerated by 9/9 Hs, 12/12 s, 2/11 s tested its formation...
Geun-Ho Kang, Yeo Kyung Shin, Min Lim, Se Jong Kim, Myeongjin Song, Kwonwoo Jung Hyun Dae Young Hang-Cheol Jin Ssang-Goo Cho. IJSC -0001;0:. https://doi.org/10.15283/ijsc24039
A 26-year-old male patient presented for stump revision of an amputated right 3rd finger and neurorrhaphy a lacerated 2nd finger. An axillary brachial plexus block was performed using the transarterial technique. Postoperatively, demonstrated signs symptoms injury. He exhibited paresthesia in distribution ulnar nerve motor weaknesses wrist, elbow, shoulder. On 18th postoperative day, conduction electromyographic studies were performed, which revealed possible radial neuropathy neuropathy, or...
Fusarim oxysporum에 의한 덩굴쪼김병은 수박 재배에 큰 피해를 일으키는 중요한 병이다. 이 병을 방제하기 위해 덩굴쪼김병 저항성 박 대목을 이용하는 것이 가장 좋은 방법이다. 본 연구는 자원을 선발할 수 있는 방법을 확립하기 수행하였다. 효과적인 접종법을 개발하기 접종 후 온도(15, 20, 25, 30°C), 농도(1 X 105, 5 1 106 and conidia･mL-1), 시기(파종 7, 10, 13, 16일 후)를 조사하였다. 덩굴쪼김병을 접종하였을 때 배양 온도나 파종 시기의 영향이 적었다. 그러나, 덩굴쪼김병에 저항성인 박의 경우 접종농도가 높을수록 발병이 심하게 나타났다. 따라서, 대한 접종법으로 10일 된 유묘 뿌리를 105 - conidia･mL-1 농도로 30분간 접종한 감염되지 않은 토양에 옮겨 심고 25°C에서 3주 동안 재배하는 것을 제안한다.