A5070150612- Fungal and yeast genetics research
- Ubiquitin and proteasome pathways
- CRISPR and Genetic Engineering
- Cellular transport and secretion
- RNA Research and Splicing
- PI3K/AKT/mTOR signaling in cancer
- Endoplasmic Reticulum Stress and Disease
- DNA Repair Mechanisms
- Pluripotent Stem Cells Research
- Listeria monocytogenes in Food Safety
- Photosynthetic Processes and Mechanisms
- Protein Kinase Regulation and GTPase Signaling
- Genetic Neurodegenerative Diseases
- 14-3-3 protein interactions
- Genomics and Chromatin Dynamics
- Viral gastroenteritis research and epidemiology
- Plant Reproductive Biology
- Mitochondrial Function and Pathology
- Plant nutrient uptake and metabolism
- RNA modifications and cancer
- Salmonella and Campylobacter epidemiology
- Developmental Biology and Gene Regulation
- Genetics, Aging, and Longevity in Model Organisms
University of Washington
2011-2024
University of California, Berkeley
2014-2020
Bowdoin College
2012
Abstract Repair of double strand DNA breaks (DSBs) can result in gene disruption or modification via homology directed repair (HDR) from donor DNA. Altering cellular responses to DSBs may rebalance editing outcomes towards HDR and away other outcomes. Here, we utilize a pooled CRISPR screen define host cell involvement between Cas9 DSB plasmid stranded (dsDonor). We find that the Fanconi Anemia (FA) pathway is required for dsDonor genes act repress HDR. Small molecule inhibition one these...
Protein quality control (PQC) degradation protects the cell by preventing toxic accumulation of misfolded proteins. In eukaryotes, PQC is primarily achieved ubiquitin ligases that attach to proteins for proteasome degradation. To function effectively, must distinguish from their normal counterparts recognizing an attribute structural abnormality commonly shared among However, nature structurally abnormal feature recognized most unknown. Here we demonstrate yeast nuclear ligase San1...
Eukaryotic ribosome biogenesis requires hundreds of trans-acting factors and dozens RNAs. Although most required for have been identified, little is known about their regulation. Here, we reveal that the yeast deubiquitinating enzyme Ubp10 localized to nucleolus ubp10Δ cells reduced pre-rRNAs, mature rRNAs, translating ribosomes. Through proteomic analyses, found interacts with proteins function in rRNA production biogenesis. In particular, discovered largest subunit RNA polymerase I (RNAPI)...
Abstract Studies from diverse systems have shown that distinct interchromosomal interactions are a central component of nuclear organization. In some cases, these allow an enhancer to act in trans, modulating the expression gene encoded on separate chromosome held close proximity. Despite recent advances uncovering such phenomena, our understanding how regulatory element acts another remains incomplete. Here, we describe transgenic approach better understand action trans Drosophila...
Transposon sequencing (Tn-seq) is a powerful genome-wide technique to assess bacterial fitness under varying growth conditions. However, screening via Tn-seq in vivo challenging. Dose limitations and host restrictions create bottlenecks that diminish the transposon mutant pool being screened. Here, we have developed murine model with disruption Akr1c13 renders resulting RECON-/- mouse resistant high-dose infection. We leveraged this perform screen of human pathogen Listeria monocytogenes...
Target of rapamycin complex-2 (TORC2), a conserved protein kinase complex, is an indispensable regulator plasma membrane homeostasis. In budding yeast (
Nine human disorders result from the toxic accumulation and aggregation of proteins with expansions in their endogenous polyalanine (polyA) tracts. Given prevalence polyA tracts eukaryotic proteomes, we wanted to understand generality polyA-expansion cytotoxicity by using yeast as a model organism. In our initial case, expanded tract within native poly(Adenine)-binding protein Pab1 8A 13A, 15A, 17A, 20A. These resulted increasing formation inclusions, insolubility, that correlated length...
Abstract Transposon sequencing (Tn-seq) is a powerful genome-wide technique to assess bacterial fitness under varying growth conditions. However, screening via Tn-seq in vivo challenging. Dose limitations and host restrictions create bottlenecks that diminish the transposon mutant pool being screened. Here we have developed murine model with disruption Akr1c13 renders resulting RECON -/- mouse resistant high dose infection. We leveraged this perform screen of human pathogen Listeria...
Repair of double strand DNA breaks (DSBs) can result in gene disruption or precise modification via homology directed repair (HDR) from a templating donor DNA. During genome editing, altering cellular responses to DSBs may be an effective strategy rebalance editing outcomes towards HDR and away other pathways. To identify factors that regulate double-stranded (dsDonor), we utilized pooled screen define the consequences thousands individual knockdowns during Cas9-initiated plasmid donor. We...
Cell survival requires preservation of plasma membrane (PM) integrity in response to environmental insults and changes lipid metabolism. In eukaryotic cells, maintenance PM homeostasis a conserved, PM‐localized protein kinase complex, Target Rapamycin (TOR) Complex 2 (TORC2). budding yeast ( Saccharomyces cerevisiae ), the essential catalytic subunit TORC2 is TOR isoform Tor2 downstream effector AGC‐family Ypk1 (and its paralog Ypk2). Our prior work has shown that phosphorylates thereby...