Abstract Papillomaviruses have evolved mechanisms that result in escape from host immune surveillance. The E5 protein is expressed early papillomavirus infection the deep layers of infected epithelium. It localized to Golgi apparatus (GA) and endoplasmic reticulum. bovine (BPV) impairs synthesis stability major histocompatibility (MHC) class I complexes prevents their transport cell surface due retention GA. Here we show human type 16 (HPV‐16) also causes MHC (HLA) GA impedes surface, which...

Abstract Human papillomavirus type 16 E5 protein (HPV‐16 E5) is expressed early in infection and localised primarily the cell Golgi apparatus (GA) endoplasmic reticulum. prevents transport of major histocompatibility class I (MHC I; HLA humans) to surface retains complex GA. We report that these effects are due, at least part, interaction between heavy chain (HC). also demonstrate down‐regulation with HC mediated by first hydrophobic domain E5. Although downregulates classical selectively as...

Tumor-associated antigens (TAAs) lead to the production of tumor-specific autoantibodies (anti-TAA autoantibodies) by triggering humoral immune system which can be used as candidate biomarkers. This study aims investigate TAAs proteins eliciting responses in different stages urothelial bladder carcinoma (UBC) using an immunoproteomics approach find novel biomarkers for clinical management disease. Total were obtained from newly established UBC cell line, JAM-ICR, and separated...

Abstract Background and Aim: Interleukin (IL)‐18 level association of its two promoter gene polymorphisms at −607C/A −137G/C positions were investigated in Iranian patients with gastrointestinal (GI) cancers. Methods: 232 cases GI cancers 312 healthy controls enrolled. Serum IL‐18 was measured by enzyme linked immunosorbent assay (ELISA) genotyping assessed allele‐specific polymerase chain reaction (PCR). Results: There a significant difference the frequency −137 G/C genotype between stomach...

Interleukin-18 (IL-18) is a multifunctional cytokine that augments IFN-gamma production and affects tumor immune response. In the present case-control study, we tested whether IL-18 promoter polymorphism contributes to lung cancer susceptibility in Iranian patients.The study groups were 73 patients with cancer, including 53 squamous carcinoma (SC) 20 small cell (SCLC), 97 healthy regional aged-matched individuals. The frequency of single nucleotide polymorphisms (SNPs) at positions -656...

The association of a functional single nucleotide polymorphism at codon 72 the p53 gene (Arg72Pro) with malignancy is subject controversy. We analyzed this in 224 patients gastrointestinal cancers (92 stomach cancer and 132 colorectal cancer) 163 healthy controls.DNA was extracted from peripheral blood mononuclear cells amplified an allele-specific polymerase chain reaction.There no significant between alleles cancers. frequency Arg allele 59.7, 58.8, 59.2% patients, controls, respectively....

To explore if the increased percentages of Regulatory T (Treg) cells, as well as, overexpression Cytotoxic T-Lymphocyte Antigen-4 (CTLA-4) are involved in laryngeal-squamous cell carcinoma (SCC), 45 patients with laryngeal-SCC and 27 healthy controls were enrolled. Flow cytometry was performed to investigate, peripheral blood, prevalence CD4+CD25+FoxP3+ Treg surface intracellular expression CTLA-4 by main lymphocyte subsets (CD4+, CD8+ CD19+). The results indicated (In)CTLA4 considerable...

Abstract Background Non-muscle-invasive bladder cancer (NMIBC) has a high recurrence rate and muscle-invasive (MIBC) unfavorable outcomes in urothelial (UBC) patients. Complex UBC-related protein biomarkers for outcome prediction may provide more efficient management approach with an improved clinical outcome. The aim of this study is to recognize tumor-associated proteins, which are differentially expressed different stages UBC patients compared non-cancerous tissues. Methods proteome...

Abstract Purpose Colon cancer is a prevalent globally, representing approximately 10% of all cases and accounting for cancer-related deaths. Therefore, finding new therapeutic methods with high efficiency will be very valuable. Cromolyn ( C ), common anti-allergic mast cell membrane stabilizing drug, has recently shown valuable anti-cancer effects in several studies. This study was designed to investigate the activity cromolyn on colon vitro vivo determine values such as selectivity index...

The objective of this study was to explore the prevalence T helper type 1 (Th1; CD4(+) IFN-γ (+) ) and Th2 (CD4(+) IL-4(+) cells, as well cytotoxic cell (Tc1; CD8(+) IFN-γ(+) Tc2 cells (CD8(+) in peripheral blood patients with salivary gland tumors (SGTs).Thirty new SGTs 15 healthy controls were recruited. After intracellular cytokine staining, data acquisition analysis performed by flow cytometry.The mean percentages Th1 Tc1 ratios Th1/Th2 Tc1/Tc2, observed be significantly lower malignant...

To determine the patterns of distribution HCV genotypes among high risk population in north Iran.A cross-sectional study was conducted on 135 RNA-positive individuals including thalassemia, hemophilia, patients under hemodialysis and intravenous drug addicts. were determined based amplification with type-specific primers methods.Among 187 anti-HCV positive samples, only (72.2%) gave HCV-RNA positvity. Over all, most identified type genotype 3a (51.1%) followed by 1a (27.4%), 1b (8.2%)....

Cervical cancer represents one of the most prevalent cancers among women worldwide, particularly in low- and middle-income nations. Oncolytic viruses (OVs) can infect cells selectively lethally without harming normal cells. Respiratory syncytial virus (RSV) is an oncolytic for anticancer therapy because its propensity to multiply within tumor This research aimed assess vitro antitumor activities molecular basis processes RSV-A2 on TC-1 as a model HPV‑related cervical cancers.Cellular...

Abstract CD11c is a member of the β2‐integrin family typically used to define myeloid dendritic cells (DCs). Recent reports identify CD11c‐expressing CD8 + T as new subset regulatory (Treg). Evidence exists that may exert their effector or functions under different conditions. To date, no studies have addressed frequency in cancer. Limited evidence terms expression immune‐checkpoint receptors, programmed cell death protein 1 (PD‐1) and T‐lymphocyte‐associated antigen 4 (CTLA‐4), well...