A5046712618- SARS-CoV-2 detection and testing
- SARS-CoV-2 and COVID-19 Research
- Biosensors and Analytical Detection
- COVID-19 Clinical Research Studies
- COVID-19 diagnosis using AI
- Respiratory viral infections research
- COVID-19 epidemiological studies
UK Health Security Agency
2022-2024
BackgroundAntigen lateral flow devices (LFDs) have been widely used to control SARS-CoV-2. We aimed improve understanding of LFD performance with changes in variant infections, vaccination, viral load, and use, the detection infectious individuals.MethodsIn this diagnostic study, paired RT-PCR test results were prospectively collected from asymptomatic symptomatic participants UK between Nov 4, 2020, March 21, 2022, support National Health Service (NHS) England's Test Trace programme. The...
The advent of lateral flow devices (LFDs) for SARS-CoV-2 detection enabled widespread use rapid self-tests during the pandemic. While self-testing using LFDs is now common, whether provides comparable performance to professional testing was a key question that remained important pandemic planning.
Abstract To detect SARS-CoV-2 amongst asymptomatic care home staff in England, a dual-technology weekly testing regime was introduced on 23 December 2020. A lateral flow device (LFD) and quantitative reverse transcription polymerase chain reaction (qRT-PCR) test were taken the same day (day 0) midweek LFD three to four days later. We evaluated effectiveness of using between 2020 April 2021. Viral concentrations derived from qRT-PCR used determine probable stage infection likely level...
Background The NHS Test and Trace (NHSTT) programme was established in May 2020 England to deliver SARS-CoV-2 testing contact tracing order identify infected individuals reduce COVID-19 spread. To further control transmission, people identified as contacts were asked self-isolate for 10 days test only if they became symptomatic. From March 2021, eligibility criteria PCR expanded include asymptomatic of confirmed cases. Aim analyse patterns before after the change guidance assess impact on...
Abstract Background Antigen lateral flow devices (LFDs) have been widely used to control SARS-CoV-2. Changes in LFD sensitivity and detection of infectious individuals during the pandemic with successive variants, vaccination, changes use are incompletely understood. Methods Paired PCR tests were collected from asymptomatic symptomatic participants, across multiple settings UK between 04-November-2020 21-March-2022. Multivariable logistic regression was analyse specificity, adjusting for...
Abstract Purpose Saliva has been considered a suitable sample material for SARS-CoV-2 testing but uncertainty remained regarding the sensitivity and reliability of different saliva collection methods community mass testing. This study aimed to investigate potential utility expectorated (ES) drooled (DS) through large cohort study. Methods ES DS samples were collected in two-stage non-randomized prospective Their RNA qRT-PCR was assessed by comparison with results combined throat nose (CTN)...
Saliva has been considered a suitable sample material for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA testing, but uncertainty remained regarding sensitivity and reliability of different saliva collection methods. This study aimed to investigate the potential utility expectorated (ES) drooled (DS) community mass testing. Self-collected ES DS samples were obtained in prospective cohort with 2,878 participants. The SARS-CoV-2 qRT-PCR testing was assessed by comparing...
The challenges of rapid upscaling testing capacity were a major lesson from the COVID-19 pandemic response. need for process adjustments in high-throughput laboratories made sample pooling challenging option to implement.This study aimed evaluate whether samples at source (swab pooling) was as effective qRT-PCR individuals identifying cases SARS-CoV-2 real-world community conditions using same pipeline.Two cohorts 10 (Pool10: 1,030 participants and 103 pools) 6 (Pool6: 1,284 214 per pool...