A5037723351- Glycosylation and Glycoproteins Research
- Electron Spin Resonance Studies
- Advanced NMR Techniques and Applications
- Protein Structure and Dynamics
- NMR spectroscopy and applications
- Carbohydrate Chemistry and Synthesis
- Chemical Synthesis and Analysis
- Ion channel regulation and function
- Molecular spectroscopy and chirality
- Venomous Animal Envenomation and Studies
- Enzyme Structure and Function
- RNA and protein synthesis mechanisms
- Computational Drug Discovery Methods
- Spectroscopy and Quantum Chemical Studies
- Monoclonal and Polyclonal Antibodies Research
- DNA and Nucleic Acid Chemistry
- HIV Research and Treatment
- Receptor Mechanisms and Signaling
- Plant-based Medicinal Research
- Solid-state spectroscopy and crystallography
- HIV/AIDS drug development and treatment
- Analytical Chemistry and Chromatography
- Bacteriophages and microbial interactions
- Synthesis and biological activity
- Biochemical and Structural Characterization
Vellore Institute of Technology University
2021
Siddhartha Medical College
2021
Liverpool Hope University
2021
National Institute Of Technology Silchar
2021
South Asian University
2021
Indian Institute of Technology Roorkee
2021
Kyungpook National University
2021
Vinayaka Missions University
2021
University of Alabama at Birmingham
2004-2019
Indian Institute of Spices Research
2017
Herein, we demonstrate that the ubiquitin-associated (UBA) domain of sequestosome 1/p62 displays a preference for binding K63-polyubiquitinated substrates. Furthermore, UBA p62 was necessary aggregate sequestration and cell survival. However, inhibition proteasome function compromised survival in cells with aggregates. Mutational analysis reveals conserved hydrophobic patch MGF as well leucine helix 2 are polyubiquitinated proteins sequestration-aggregate formation. We report interacts by...
Reactive nitrogen species derived from nitric oxide are potent oxidants formed during inflammation that can oxidize membrane and lipoprotein lipids in vivo. Herein, it is demonstrated several of these react with unsaturated fatty acid to yield nitrated oxidation products. Using HPLC coupled both UV detection electrospray ionization mass spectrometry, products reaction ONOO- linoleic displayed mass/charge (m/z) characteristics LNO2 (at least three at m/z 324, negative ion mode). Further...
Spores of Bacillus anthracis, the causative agent anthrax, are enclosed by a prominent loose fitting layer called exosporium. The exosporium consists basal and an external hairlike nap. filaments nap composed highly immunogenic glycoprotein BclA, which has long, central collagen-like region with multiple XXG repeats. Most triplet repeats PTG, nearly all contain threonine residue, providing potential sites for O-glycosylation. In this study, we demonstrated that two O-linked oligosaccharides,...
Conjugates of curcumin to two differently sized poly(ethylene glycol) molecules were synthesized in an attempt overcome the low aqueous solubility this natural product with cytotoxic activity against some human cancer cell lines. The soluble conjugates exhibited enhanced cytotoxicity as compared that parent drug. Synthesis, analyses rate drug release, and studies are herein reported. water-soluble may provide information useful for development injectable conjugates.
Nitric oxide ( • NO) and NO-derived reactive species rapidly react with lipids during both autocatalytic enzymatic oxidation reactions to yield nitrated derivatives that serve as cell signaling molecules. Herein we report the synthesis, purification, characterization, bioactivity of nitrolinoleate (LNO 2 ). Nitroselenylation linoleic acid yielded LNO was purified by solvent extraction, silicic chromatography, reverse-phase HPLC. Structural characterization performed IR spectroscopy, 15...
The analysis of virus-receptor interactions at atomic resolution is fundamental importance to understand infection processes, and establish novel anti-viral therapies. As an example, rabbit hemorrhagic disease virus (RHDV), a member the Caliciviridae family considered as "emerging" virus, attaches histo-blood group antigens (HBGA) on surface adult epithelial cells upper respiratory digestive tracts. It appears that this attachment key step in process with RHDV. Here, we report NMR...
The biosynthesis of human blood group B antigens is accomplished by a highly specific galactosyltransferase (GTB). On the basis NMR experiments, we propose "molecular tweezers mechanism" that accounts for exquisite stereoselectivity donor substrate selection. Transferred NOE experiments first time reveal bioactive conformation UDP-galactose (UDP-Gal) and its enzymatically inactive analogue, UDP-glucose (UDP-Glc). Both bind to GTB in folded sparsely populated solution, whereas acceptor...
Class A amphipathic helical peptides have been shown to mimic apolipoprotein A-I, the major protein component of high density lipoproteins and inhibit atherosclerosis in several dyslipidemic mouse models. Previously we reported NMR structure Ac-18A-NH2, base-line model class peptide a 50% (v/v) trifluoroethanol-d3/water mixture, membrane-mimic environment (Mishra, V. K., Palgunachari, M. N., Anantharamaiah, G. M., Jones, Segrest, J. P., Krishna, N. R. (2001) Peptides 22, 567-573). The...
As in other retroviruses, the HIV-1 capsid (CA) protein is composed of two domains, N-terminal domain (NTD) and C-terminal (CTD), joined by a flexible linker. The dimerization CTD thought to be critical step assembly immature mature viral capsids. precise nature functional form interface has been subject considerable interest. Previously, dimer was involve face-to-face observed early crystallographic studies. Recently, structure for domain-swapped determined. This dimer, with an entirely...
This report describes the use of concept inversion hydropathy patterns to de novo design peptides targeted a predetermined site on protein. Eight- and 12-residue were constructed with EF hands or Ca2+-coordinating sites calmodulin as their anticipated points interaction. These peptides, but not unrelated nor those same amino acid composition scrambled sequence, interacted two carboxyl-terminal Ca2+-binding well troponin C. The interactions resulted in conformational change whereby 8-mer...
The STD NMR technique has originally been described as a tool for screening large compound libraries to identify the lead compounds that are specific target proteins of interest. application this in qualitative epitope mapping ligands weakly binding proteins, virus capsid shells, and nucleic acids also described. Here we describe intensity-restrained CORCEMA optimization (SICO) procedure refining bound conformation UDP−galactose galactosyltransferase complex using intensities recorded at 500...
Abstract Two‐dimensional nuclear Overhauser enhancement (2D NOESY) data are reported for the polypentapeptide of elastin, poly(VPGVG), and cyclopentadecapeptide, cyclo(VPGVG) 3 . In both, repeating type II Pro 2 ‐Gly β‐turn can be derived from NOE data, providing confirmation many previous studies. addition, other through‐space connectivities detailed that also compare favorably with previously determined crystal solution structures Also, near identical cyclopentadecapeptide demonstrate...
The capsid protein (CA) of HIV-1 plays a significant role in the assembly immature virion and is critical building block its mature capsid. Thus, there has been interest CA as target design inhibitors early late stage events replication cycle. However, because inherent flexibility from interdomain linker monomer–dimer equilibrium solution, wild-type monomer defied structural determinations by X-ray crystallography nuclear magnetic resonance spectroscopy. Here we report detailed solution...