A5037890718- Retinoids in leukemia and cellular processes
- Virus-based gene therapy research
- Ubiquitin and proteasome pathways
- Clostridium difficile and Clostridium perfringens research
- RNA Research and Splicing
- Plant-based Medicinal Research
- Biotin and Related Studies
- bioluminescence and chemiluminescence research
- Botulinum Toxin and Related Neurological Disorders
- interferon and immune responses
- Protein Degradation and Inhibitors
- Probiotics and Fermented Foods
- Diphtheria, Corynebacterium, and Tetanus
- Click Chemistry and Applications
- RNA Interference and Gene Delivery
- Herpesvirus Infections and Treatments
- Bacterial Identification and Susceptibility Testing
- Nuclear Structure and Function
- Cancer Treatment and Pharmacology
- Bacterial Infections and Vaccines
- HIV Research and Treatment
- Acute Myeloid Leukemia Research
- Toxin Mechanisms and Immunotoxins
- Immunotoxicology and immune responses
- Viral Infectious Diseases and Gene Expression in Insects
Centre National de la Recherche Scientifique
1996-2023
Université Paris Cité
2011-2023
Inserm
1992-2023
Centre Interdisciplinaire de Recherche en Biologie
2021-2023
Collège de France
2021-2023
Université Paris Sciences et Lettres
2023
Hôpital Saint-Louis
2003-2021
University of London Institute in Paris
2020
La Ligue Contre le Cancer
2020
Délégation Paris 5
2011-2016
Key components of the miRNA-mediated gene regulation pathway are localized in cytoplasmic processing bodies (P-bodies). Mounting evidence suggests that presence microscopic P-bodies not always required for regulation. Here we have shown geldanamycin, a well-characterized HSP90 inhibitor, abolishes and significantly reduces Argonaute GW182 protein levels but does affect miRNA level efficiency repression; however, it impairs siRNA loading efficacy exogenous siRNA. Our data chaperones...
ABSTRACT The use of live microorganisms as an antigen delivery system is effective means to elicit local immune responses and thus represents a promising strategy for mucosal vaccination. In this respect, lactic acid bacteria represent original attractive approach, they are safe organisms that used food starters probiotics. To determine whether response could be elicited by intranasal recombinant lactobacilli, Lactobacillus plantarum strain human origin (NCIMB8826) was selected the...
ABSTRACT The potential of lactic acid bacteria as live vehicles for the production and delivery therapeutic molecules is being actively investigated today. For future applications it essential to be able establish dose-response curves targeted biological effect thus control a heterologous biopeptide by lactobacillus. We therefore implemented in Lactobacillus plantarum NCIMB8826 powerful nisin-controlled expression (NICE) system based on autoregulatory properties bacteriocin nisin, which...
The modification of proteins by SUMO (small ubiquitin-related modifier) plays important roles in regulating the activity, stability and cellular localization target proteins. Similar to ubiquitination, is a dynamic process that can be reversed SENPs [SUMO-1/sentrin/SMT3 (suppressor mif two 3 homologue 1)-specific peptidases]. To date, six have been discovered humans, although knowledge their regulation, specificity biological functions limited. In present study, we report SENP7 has...
Abstract PML nuclear bodies (NB) are disrupted in PML-RARA–driven acute promyelocytic leukemia (APL). Arsenic trioxide (ATO) cures 70% of patients with APL, driving PML-RARA degradation and NB reformation. In non-APL cells, arsenic binding onto also amplifies formation. Yet, the actual molecular mechanism(s) involved remain(s) elusive. Here, we establish that NBs display some features liquid–liquid phase separation ATO induces a gel-like transition. B-box-2 structure reveals an alpha helix...
ABSTRACT The lactic acid bacteria (LAB) are safe microorganisms which mainly used for the preparation of fermented foods and probiotic applications. potential LAB as live vehicles production delivery therapeutic molecules such antigens is also being actively investigated today. However, very little known about fate when administered in vivo interaction these with nasal or gastrointestinal ecosystem. For future applications, it essential to be able discriminate biotherapeutic strain from...
The biologic role of a majority the Neisseria meningitidis 2100 predicted coding regions is still to be assigned or experimentally confirmed. Determining phenotypic effect gene disruption being fundamental approach understanding function, we used high-density signature-tagged transposon mutagenesis, followed by large-scale sequencing insertion sites, construct genome-wide collection mutants. results for first half 4548 mutants composing library suggested that have mutations in 80%–90% N....
In acute promyelocytic leukemia (APL), the (PML) protein is fused to retinoic acid receptor alpha (RAR). Arsenic an effective treatment for this disease as it induces SUMO-dependent ubiquitin-mediated proteasomal degradation of PML-RAR fusion protein. Here we analyze nuclear trafficking dynamics PML and its ubiquitin E3 ligase, RNF4 in response arsenic. After administration arsenic, immediately transits into bodies where undergoes SUMO modification. This initial recruitment not dependent on...
Nup98 is a glycine-leucine-phenylalanine-glycine (GLFG) repeat–containing nucleoporin that, in addition to nuclear transport, contributes multiple aspects of gene regulation. Previous studies revealed its dynamic localization within intranuclear structures known as GLFG bodies. Here we show that the mammalian Nup107-160 complex (Y-complex), major scaffold module pore, together with partner Elys, colocalizes The frequency and size bodies vary among HeLa sublines, find an increased level...
Abstract Membrane-less organelles are condensates formed by phase separation whose functions often remain enigmatic. Upon oxidative stress, PML scaffolds Nuclear Bodies (NBs) to regulate senescence or metabolic adaptation. NBs recruit many partner proteins, but the actual biochemical mechanism underlying their pleiotropic remains elusive. Similarly, role in embryonic stem cell (ESC) and retro-element biology is unsettled. Here we demonstrate that essential for stress-driven SUMO2/3...
Abstract: Senile plaques are neuropathological manifestations in Alzheimer's disease (AD) and composed mainly of extracellular deposits amyloid β‐peptide (Aβ). Various data suggest that the accumulation Aβ may contribute to neuronal degeneration neurotoxicity could be mediated by oxygen free radicals. Removal radicals antioxidant scavengers or enzymes was found protect cells culture from toxicity. However, nature involved is still unclear. In this study, we investigated whether...
Multiple cellular pathways are regulated by small ubiquitin-like modifier (SUMO) modification, including ubiquitin-mediated proteolysis, signal transduction, innate immunity, and antiviral defense. In the study described in this report, we investigated effects of SUMO on replication two members Rhabdoviridae family, vesicular stomatitis virus (VSV) rabies (RABV). We show that stable expression human cells confers resistance to VSV infection an interferon-independent manner. demonstrate did...
Adeno-associated virus type 2 (AAV-2) is a human parvovirus that requires the presence of helper virus, such as herpes simplex 1 (HSV-1) to accomplish complete productive cycle. In absence AAV-2 can establish latent infection characterized by expression viral genes. So far, four HSV-1 early genes, UL5/8/52 (helicase primase complex) and UL29 (single-stranded DNA-binding protein), were defined sufficient for AAV replication when cells transfected with plasmid carrying wild-type genome....