A5025351503- Marine Toxins and Detection Methods
- Marine and coastal ecosystems
- Protist diversity and phylogeny
- Marine Biology and Environmental Chemistry
- Nicotinic Acetylcholine Receptors Study
- Marine Sponges and Natural Products
- Aquatic Ecosystems and Phytoplankton Dynamics
- Environmental Toxicology and Ecotoxicology
- Molecular Sensors and Ion Detection
- Environmental Chemistry and Analysis
- Synthetic Organic Chemistry Methods
- Microbial Community Ecology and Physiology
- Metabolomics and Mass Spectrometry Studies
- Axial and Atropisomeric Chirality Synthesis
- Algal biology and biofuel production
- Mass Spectrometry Techniques and Applications
- Neuroscience and Neuropharmacology Research
- Chemical synthesis and alkaloids
- Receptor Mechanisms and Signaling
- Water Quality Monitoring and Analysis
- Oxidative Organic Chemistry Reactions
- Animal Ecology and Behavior Studies
- Microplastics and Plastic Pollution
- Neurotransmitter Receptor Influence on Behavior
- Vanadium and Halogenation Chemistry
University of Naples Federico II
2015-2024
University of Palermo
2023-2024
CoNISMa
2018-2022
Federico II University Hospital
2015
University of Trieste
2009
United States Army Medical Research Institute of Infectious Diseases
2009
Panthera Biopartners
2009
Institute for Marine Biosciences
2002-2008
National Research Council Canada
2002-2008
Consorzio Interuniversitario Nazionale La Chimica Per L'Ambiente
2007
Harmful cyanobacterial blooms, which frequently contain toxic secondary metabolites, are reported in aquatic environments around the world. More than two thousand metabolites have been from diverse sources over past fifty years. A comprehensive, publically-accessible database detailing these would facilitate research into their occurrence, functions and toxicological risks. To address this need we created CyanoMetDB, a highly curated, flat-file, openly-accessible of collated 850...
A new method for sensitive, specific, and direct determination of palytoxin is proposed herein. It based on combination reversed-phase liquid chromatography with mass spectrometry (LC-MS). The was set up a turbo ion spray-triple quadrupole MS instrument operating in selected monitoring (SIM) multiple reaction (MRM) acquisition modes (positive ions). minimum detection levels matrix-free toxin column were thus estimated from the data to be 200 125 pg SIM MRM modes, respectively. Spiking...
In this article we report on the liquid chromatography tandem mass spectrometry (LC-MS) investigation of plankton samples collected in summer 2006 along Ligurian coasts, coinciding with a massive bloom tropical microalga Ostreopsis ovata. LC-MS analyses indicated occurrence putative palytoxin much more abundant palytoxin-like compound never reported so far, which named ovatoxin-a. On basis molecular formula, fragmentation pattern, and chromatographic behavior, structure ovatoxin-a appeared...
Abstract Over the past decades, Italian coastlines have been plagued by recurring presence of benthic dinoflagellate Ostreopsis ovata . Such an alga has caused severe sanitary emergencies and economic losses due to its production palytoxin‐like compounds. Previous studies confirmed ovatoxin‐a (OVTX‐a) as major toxin algal profile together with small amounts putative palytoxin (PLTX). In our ongoing research on O toxins we report herein in‐depth investigation culture carried out...
Since 2005, the benthic dinoflagellate Ostreopsis cf. ovata has bloomed across Mediterranean basin, provoking serious toxic outbreaks. LC/MS studies have identified a number of palytoxin-like compounds, termed ovatoxins, along with trace amounts putative palytoxin as causative agents O. -related human sufferings. So far, any risk assessment for ovatoxins well establishment their allowance levels in seafood been prevented by lack pure toxins. The present paper reports on isolation, NMR-based...
Since the late 1990s, a respiratory syndrome has been repetitively observed in humans concomitant with Ostreopsis spp. blooms (mainly O. cf. ovata) Mediterranean area. Previous studies have demonstrated that ovata produces analogues of palytoxin (ovatoxins and putative palytoxin), one most potent marine toxins. On basis association between blooms, illness people, detection complex algal samples, toxic aerosols, containing cells and/or toxins they produce, were postulated to be cause human...
Chemical analyses of plankton and highly toxic mussel samples collected in eastern Canada during an intense bloom the dinoflagellate Alexandrium tamarense established presence a complex mixture paralytic shellfish poisoning (PSP) toxins. Application newly developed technique, hydrophilic interaction liquid chromatography−mass spectrometry, confirmed identities known toxins revealed mussels five saxitoxin analogues (M1−M5) that were not present plankton. Four these compounds isolated their...
Currently, the benthic dinoflagellate Ostreopsis cf. ovata represents a serious concern to human health in whole Mediterranean basin due production of palytoxin congeners, putative and ovatoxins (ovatoxin-a, -b, -c, -d/-e), listed among most potent marine toxins. High resolution liquid chromatography-mass spectrometry (HR LC-MS) based investigation North Western Adriatic strain collected at Portonovo (Italy) 2008 is reported herein. Toxin profile was different from those previously for other...
The new benthic toxic dinoflagellate, Ostreopsis fattorussoi sp. nov., is described from the Eastern Mediterranean Sea, Lebanon and Cyprus coasts, supported by morphological molecular data. plate formula, Po, 3', 7″, 6c, 7s, 5‴, 2'''', typical for genus. It differs all other species in that (i) curved suture between plates 1' 3' makes them approximately hexagonal, (ii) lies left half of epitheca obliquely orientated leading to a characteristic shape 6″. round thecal pores are bigger than two...
This paper reports on the analysis of toxin content from Palythoa tuberculosa and toxica samples collected off Hawaiian coast. Our work, based in-depth high-resolution liquid chromatography−mass spectrometry along with extensive NMR study, led us to structurally characterize 42-hydroxy-palytoxin, a new palytoxin congener. itself appeared be major components toxic extract P. sample, while 42-hydroxy-palytoxin was proven by far main derivative in toxica. Functional studies this palytoxin-like...