Summary Trypanosomes are outstanding examples of the importance mRNA metabolism in regulation gene expression, as these unicellular eukaryotes mostly control protein synthesis by post‐transcriptional mechanisms. Here, we show that organisms involves recruitment mRNAs and proteins to microscopically visible ribonucleoprotein granules cytoplasm. These structures engage transcripts being translated protect from degradation. Analysis composition trypanosomal indicated they contain orthologous...

The protist parasite Trypanosoma cruzi has evolved the ability to transit between completely different hosts and replicate in adverse environments. In particular, epimastigote form, replicative stage inside vector, is subjected nutritional osmotic stresses during its development. this work, we describe biochemical global gene expression changes of epimastigotes under hyperosmotic conditions. Hyperosmotic stress resulted cell shrinking within a few minutes. Depending on medium osmolarity, was...

Trypanosoma cruzi , the causative agent of Chagas disease, presents a major public health challenge in Central and South America, affecting approximately 8 million people placing millions more at risk. The T. life cycle includes transitions between epimastigote, metacyclic trypomastigote, amastigote, blood trypomastigote stages, each marked by distinct morphological molecular adaptations to different hosts environments. Unlike other trypanosomatids, does not employ antigenic variation but...

Trypanosoma cruzi , the causative agent of Chagas disease, presents a major public health challenge in Central and South America, affecting approximately 8 million people placing millions more at risk. The T. life cycle includes transitions between epimastigote, metacyclic trypomastigote, amastigote, blood trypomastigote stages, each marked by distinct morphological molecular adaptations to different hosts environments. Unlike other trypanosomatids, does not employ antigenic variation but...

Trypanosoma cruzi , the causative agent of Chagas disease, presents a major public health challenge in Central and South America, affecting approximately 8 million people placing millions more at risk. The T. life cycle includes transitions between epimastigote, metacyclic trypomastigote, amastigote, blood trypomastigote stages, each marked by distinct morphological molecular adaptations to different hosts environments. Unlike other trypanosomatids, does not employ antigenic variation but...

Trypanosomes are unique eukaryotic cells, in that they virtually lack mechanisms to control gene expression at the transcriptional level. These microorganisms mostly protein synthesis by posttranscriptional regulation processes, like mRNA stabilization and degradation. Transcription these cells is polycistronic. Tens hundreds of protein-coding genes unrelated function arrayed long clusters on same DNA strand. Polycistrons cotranscriptionally processed trans-splicing 5' end polyadenylation 3'...

Trypanosomatids are parasites of health importance that cause neglected diseases in humans and animals. Chagas’ disease, caused by Trypanosoma cruzi , affects 6–7 millions people worldwide, mostly Latin America, most whom do not have access to diagnosis or treatment. Currently, there no available vaccines, the antiparasitic drugs used for treatment often toxic ineffective chronic stage infection. Therefore, exploration new therapeutic targets is necessary highlights identifying options this...

Trypanosomes mostly control gene expression by post-transcriptional events such as modulation of mRNA stability and translational efficiency. These mechanisms involve RNA-binding proteins (RBPs), which associate with transcripts to form messenger ribonucleoprotein (mRNP) complexes. In this study, we report the identification targets for Trypanosoma cruzi U-rich RBP 1 (Tc UBP1) T. 3 RBP3), two phylogenetically conserved among Kinetoplastids. Co-immunoprecipitated RBP-associated RNAs were...

Trypanosomes, protozoan parasites from the order Kinetoplastida, have to deal with environmental changes during interaction their hosts. Trypanosoma cruzi, causative agent of Chagas' disease, uses post-transcriptional mechanisms regulate gene expression. However, few RNA-binding proteins involved in mRNA turnover control been identified date. In this work, an RNA recognition motif (RRM)-type protein family named T. cruzi (TcRBP) and composed at least six members was identified. The genomic...

Trypanosoma cruzi, the agent of Chagas disease, does not seem to control gene expression through regulation transcription initiation and makes use post-transcriptional mechanisms. We report here a 43-nt U-rich RNA element located in 3'-untranslated region (3'-UTR) large number T. cruzi mRNAs that is important for mRNA abundance intracellular amastigote stage parasite. Whole genome scan analysis, differential display RT-PCR, Northern blot, RT-PCR analyses were used determine transcript levels...

Background During its life cycle, the human pathogen Trypanosoma cruzi must quickly adapt to different environments, in which variation gene expression of regulatory U-rich RNA-binding protein 1 (TcUBP1) plays a crucial role. We have previously demonstrated that overexpression TcUBP1 insect-dwelling epimastigotes orchestrates an RNA regulon promote differentiation infective forms. Methods In attempt generate knockout parasites by using CRISPR-Cas9 technology, present study, we obtained...

The regulation of transcription in trypanosomes is unusual. To modulate protein synthesis during their complex developmental stages, these unicellular microorganisms rely largely on post-transcriptional gene expression pathways. These pathways include a plethora RNA-binding proteins (RBPs) that all steps the mRNA life cycle and help organize transcriptomes into clusters regulons. aim this work was to characterize an RNA regulon comprising numerous transcripts trypomastigote-associated...

SUMOylation is a post-translational modification conserved in eukaryotic organisms that involves the covalent attachment of small ubiquitin-like protein SUMO to internal lysine residues target proteins. This tag usually alters interaction surface modified and can be translated into changes its biological activity, stability or subcellular localization, among other possible outputs. attached as single moiety polymers case there are acceptor sites itself. These chains have been shown important...

Abstract Trypanosomes regulate gene expression mostly by posttranscriptional mechanisms, including control of mRNA turnover and translation efficiency. This regulation is carried out via certain elements located at the 3′‐untranslated regions s, which are recognized RNA ‐binding proteins. In trypanosomes, trans‐splicing central importance to maturation. We have previously shown that Tc DRBD 4/ PTB 2, a trypanosome homolog human polypyrimidine tract‐binding protein splicing regulator,...

We present data on the proteome of Trypanosoma cruzi epimastigote cells overexpressing U-rich RNA-binding protein 1 (UBP1). The role this regulatory during epimastigote-to-metacyclic trypomastigote stage transition was clearly established by our group at transcriptome level; nevertheless, impact UBP1 overexpression synthesis is not known. To address question, we performed shotgun label-free quantification proteomics using an in vitro system based tetracycline-inducible expression TcUBP1 and...

Previous studies have shown that overexpression of the Trypanosoma cruzi U-rich RNA-binding protein 1 (TcUBP1) in insect-dwelling epimastigotes results a gene expression pattern resembling infective form pathogen. Here, we used CRISPR-Cas9-induced edition TcUBP1 and full-length epimastigote cells to monitor transcriptomic changes during epimastigote-to-metacyclic trypomastigote stage transition T. cruzi. This dataset includes bioinformatics analysis three different RNA-seq samples, each with...

ABSTRACT During its life cycle, the human pathogen Trypanosoma cruzi must quickly adapt to different environments, in which variation gene expression of regulatory U-rich RNA-binding protein 1 (TcUBP1) plays a crucial role. We have previously demonstrated that overexpression TcUBP1 insect-dwelling epimastigotes orchestrates an RNA regulon promote differentiation infective forms. In attempt generate knockout parasites by using CRISPR-Cas9 technology, present study, we obtained variant...