A5046984456- Electrospun Nanofibers in Biomedical Applications
- Bee Products Chemical Analysis
- Tissue Engineering and Regenerative Medicine
- Connective Tissue Growth Factor Research
- Wound Healing and Treatments
- Healthcare and Venom Research
- Connective tissue disorders research
- Wnt/β-catenin signaling in development and cancer
- Periodontal Regeneration and Treatments
- Muscle Physiology and Disorders
- Additive Manufacturing and 3D Printing Technologies
- Kruppel-like factors research
- Exercise and Physiological Responses
- Essential Oils and Antimicrobial Activity
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Genomics and Chromatin Dynamics
- Electrohydrodynamics and Fluid Dynamics
- S100 Proteins and Annexins
- Moyamoya disease diagnosis and treatment
- Medical and Biological Ozone Research
- Insect and Pesticide Research
- Mesenchymal stem cell research
- Silk-based biomaterials and applications
- Tendon Structure and Treatment
- Intracranial Aneurysms: Treatment and Complications
St. Jude Children's Research Hospital
2021-2023
Molecular Oncology (United States)
2023
University of Memphis
2017-2022
Saint Louis University
2015-2019
St Louis Community College
2019
Bilkent University
2017
Abstract Skeletal muscle regeneration involves coordinated interactions between different cell types. Injection of platelet-rich plasma is circumstantially considered an aid to repair but whether platelets promote beyond their role in hemostasis remains unexplored. Here, we find that signaling via platelet-released chemokines early event necessary for mice. Platelet depletion reduces the levels platelet-secreted neutrophil chemoattractants CXCL5 and CXCL7/PPBP. Consequently, early-phase...
Recent work has shown that Manuka honey, an increasingly popular wound additive with potent antibacterial properties, also anti-inflammatory properties. However, little research been done examining its effect on neutrophils. This study investigates the hypothesis honey reduces neutrophil superoxide release and chemotaxis activation of inflammatory nuclear factor- κ B (NF- B) signaling pathway under honey’s cytotoxic limit. A differentiated HL-60 cell line was used as a model cultured in...
Metastases arise from rare cancer cells that successfully adapt to the diverse microenvironments encountered during dissemination through bloodstream and colonization of distant tissues. How acquire ability appropriately respond microenvironmental stimuli remains largely unexplored. Here, we report an epigenetic pliancy mechanism allows metastasize. We find a decline in activity transcriptional repressor ZBTB18 defines metastasis-competent mouse models. Restoration reduces chromatin...
This study focused on the characterization of Manuka honey-containing poly(ε-caprolactone) (PCL) nanofiber scaffolds with regards to wound healing. Scaffolds were electrospun from 1, 5, 10, and 20% v/v honey solutions. subjected ethanol disinfection soaked in phosphate-buffered saline (PBS) for various timepoints, scaffold morphology release was quantified. showed increased water vapor transmission rate (WVTR) soak time, indicating an increase evaporation due enhanced osmotic potential...
Abstract Platelets have important hemostatic functions in repairing blood vessels upon tissue injury. Cytokines, growth factors, and metabolites stored platelet α‐granules dense granules are released activation clotting. Emerging evidence indicates that such platelet‐derived signaling factors instrumental guiding regeneration. Here, we discuss the roles of platelet‐secreted skeletal muscle Chemokines secreted by platelets early phase after injury needed to recruit neutrophils injured...
Abstract Collagen remodeling contributes to many physiologic and pathologic processes. In primary tumors, the linearization of collagen fibers promotes cancer cell invasion metastasis is indicative poor prognosis. However, it remains unknown whether there are endogenous inhibitors that could be exploited therapeutically. Here, we show controlled by two secreted matricellular proteins with antagonistic functions. Specifically, WISP1 was cells, bound type I (Col I), linearized Col via its...
A large body of in vivo and vitro evidence indicates that Manuka honey resolves inflammation promotes healing when applied topically to a wound. In this study, the effect two different concentrations (0.5% 3% v/v) on release cytokines, chemokines, matrix-degrading enzymes from neutrophils was examined using differentiated HL-60 cell line model presence inflammatory stimuli. The results indicate 0.5% decreased TNF-α, IL-1β, MIP-1α, MIP-1β, IL-12 p70, MMP-9, MMP-1, FGF-13, IL-1ra, IL-4...
Manuka honey, a topical wound treatment used to eradicate bacteria, resolve inflammation, and promote healing, is focus in the tissue engineering community as template additive. However, its effect on neutrophil extracellular trap formation (NETosis) has yet be examined. As NETosis been implicated chronic inflammation fibrosis, reduction this response within environment of interest. In study, honey was incorporated into electrospun templates with large (1.7–2.2 µm) small (0.25–0.5 diameter...
Abstract Manuka honey is an ancient remedy to improve wound healing; however, effective delivery system needed facilitate extended release of into wounds. We developed electrospun dermal regeneration template consisting a poly (ε‐caprolactone) (PCL) scaffold embedded with 1%, 5%, 10%, or 20% manuka honey. In vitro studies demonstrated that PCL scaffolds were not toxic macrophages, and they allowed for macrophage infiltration the scaffolds. Vascular endothelial growth factor (VEGF), marker...
Abstract The development of pressure ulcers in spinal cord injury patients is extremely common, often requiring extensive surgical procedures. Macrophages (MACs) play a crucial role the innate immune system, contributing to wound healing and overall regeneration. MACs have been found possess potential be activated by external factors from their M0 inactive state an M1 proinflammatory or M2 regenerative state. This study conducted comprehensive evaluation MAC phenotype response electrospun...
Abstract Although electrospun templates are effective at mimicking the extracellular matrix (ECM) of native tissue due to tailorability parameters such as fiber diameter, polymer composition, and drug loading, these often limited with regards cell infiltration microenvironments within structures. Thus, there remains a need for flexible threedimensional template system which could be combined suspensions promote three-dimensional regeneration, ultimately allow cells freely reorganize modify...
<p>Supplementary Materials and Methods; Supplementary Figures S1 to S8; Figure S1. WISP2 inhibits WISP1-induced Col I linearization; S2. is less abundant in tumors than adjacent normal tissues an inhibitor of tumor cell invasion though I; S3. The C-terminal domain WISP1 drives through but dispensable for WISP1-Col binding; S4. Wisp1 expressed by cells cancer-associated fibroblast (CAF)-enriched stromal isolated from primary breast tumors; S5. WISP1DCT block TGFb1-induced acting as...
<div>Abstract<p>Collagen remodeling contributes to many physiologic and pathologic processes. In primary tumors, the linearization of collagen fibers promotes cancer cell invasion metastasis is indicative poor prognosis. However, it remains unknown whether there are endogenous inhibitors that could be exploited therapeutically. Here, we show controlled by two secreted matricellular proteins with antagonistic functions. Specifically, WISP1 was cells, bound type I (Col I),...
<div>Abstract<p>Collagen remodeling contributes to many physiologic and pathologic processes. In primary tumors, the linearization of collagen fibers promotes cancer cell invasion metastasis is indicative poor prognosis. However, it remains unknown whether there are endogenous inhibitors that could be exploited therapeutically. Here, we show controlled by two secreted matricellular proteins with antagonistic functions. Specifically, WISP1 was cells, bound type I (Col I),...
<p>Supplementary Materials and Methods; Supplementary Figures S1 to S8; Figure S1. WISP2 inhibits WISP1-induced Col I linearization; S2. is less abundant in tumors than adjacent normal tissues an inhibitor of tumor cell invasion though I; S3. The C-terminal domain WISP1 drives through but dispensable for WISP1-Col binding; S4. Wisp1 expressed by cells cancer-associated fibroblast (CAF)-enriched stromal isolated from primary breast tumors; S5. WISP1DCT block TGFb1-induced acting as...