A5015723475- CRISPR and Genetic Engineering
- Pluripotent Stem Cells Research
- Genomics and Phylogenetic Studies
- Genetic diversity and population structure
- Evolution and Genetic Dynamics
- Plant Disease Resistance and Genetics
- Chromosomal and Genetic Variations
- Fungal and yeast genetics research
- Virus-based gene therapy research
- Cardiac electrophysiology and arrhythmias
- Ion channel regulation and function
- Cardiovascular Effects of Exercise
- Neuroscience and Neural Engineering
National Heart Lung and Blood Institute
2019-2022
National Institutes of Health
2019-2022
University of Washington
2019-2021
Human Genome Sciences (United States)
2018
Barbara McClintock first hypothesized that interspecific hybridization could provide a "genomic shock" leads to the mobilization of transposable elements (TEs). This hypothesis is based on idea regulation TE movement potentially disrupted in hybrids. However, handful studies testing this have yielded mixed results. Here, we set out identify if can increase transposition rate and facilitate colonization TEs Saccharomyces cerevisiae × uvarum yeast small number active long terminal repeat...
Embryos devoid of autonomic innervation suffer sudden cardiac death. However, whether neurons have a role in heart development is poorly understood. To investigate if sympathetic impact cardiomyocyte maturation, we co-cultured phenotypically immature cardiomyocytes derived from human induced pluripotent stem cells with mouse ganglion neurons. We found that 1) multiple structure and ion channel genes related to maturation were up-regulated when neurons; 2) sarcomere organization connexin-43...
Interspecific hybridization can introduce genetic variation that aids in adaptation to new or changing environments. Here, we investigate how hybrid temperature and nutrient limitation may alter parental genome representation over time. We evolved Saccharomyces cerevisiae x uvarum hybrids nutrient-limited continuous culture at 15°C for 200 generations. In comparison previous evolution experiments 30°C, identified a number of responses only observed the colder regime, including loss S. allele...
Human induced pluripotent stem cells (iPSCs) that express stable and robust fluorescent proteins have proven to be indispensable in basic translational research. These reporter iPSC lines can greatly facilitate cell imaging, sorting, tracking vitro vivo studies. Here, we document two human generated by gene-editing technologies precisely integrated one-copy of a tdTomato transgene driven strong CAG promoter into the AAVS1 safe harbor locus.
Abstract Interspecific hybridization can introduce genetic variation that aids in adaptation to new or changing environments. Here we investigate how the environment, and more specifically temperature, interacts with hybrid genomes alter parental genome representation over time. We evolved Saccharomyces cerevisiae x uvarum hybrids nutrient-limited continuous culture at 15°C for 200 generations. In comparison previous evolution experiments 30°C, identified a number of temperature specific...
Human-derived induced pluripotent stem cells (iPSCs) have proven to be indispensable in cardiovascular drug development, disease modeling, and developmental biology research. For this reason, it is particularly useful develop wild-type iPSC lines used experimental or control conditions. Here, we present two such cell generated from a sample of peripheral blood mononuclear (PBMCs) healthy patient with normal cardiac function.
Abstract Barbara McClintock first hypothesized that interspecific hybridization could provide a “genomic shock” leads to the mobilization of transposable elements. This hypothesis is based on idea regulation element movement potentially disrupted in hybrids. However, handful studies testing this have yielded mixed results. Here, we set out identify if can increase transposition rate and facilitate colonization elements Saccharomyces cerevisiae x uvarum yeast S. small number active long...