A5077824773- Influenza Virus Research Studies
- T-cell and Retrovirus Studies
- Animal Disease Management and Epidemiology
- Viral gastroenteritis research and epidemiology
- Bacteriophages and microbial interactions
- Respiratory viral infections research
- Vector-Borne Animal Diseases
- Poxvirus research and outbreaks
- Bacillus and Francisella bacterial research
- Infection Control and Ventilation
- Animal Virus Infections Studies
- Viral Infections and Outbreaks Research
- SARS-CoV-2 and COVID-19 Research
- SARS-CoV-2 detection and testing
- Herpesvirus Infections and Treatments
- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- Muscle Physiology and Disorders
- Viral Infections and Immunology Research
- Gene expression and cancer classification
- Biochemical and Structural Characterization
- Virus-based gene therapy research
- Climate variability and models
- Breast Lesions and Carcinomas
- Ubiquitin and proteasome pathways
Université Paris Cité
2006-2023
Institut Pasteur
2010-2023
Délégation Paris 7
2010-2018
Sorbonne Paris Cité
2011-2018
University of Mons
2006-2009
University of Cambridge
2007
Centre National de la Recherche Scientifique
2003-2005
Université Claude Bernard Lyon 1
2003
Centre Léon Bérard
2003
Laboratoire de Biologie Moléculaire et Cellulaire des Eucaryotes
2001
Cell culture medium, nasopharyngeal and sera samples spiked with SARS-CoV-2 were subjected to heat inactivation for various periods of time, ranging from 30 s 60 min. Our results showed that could be inactivated in less than min, 15 3 min at 56 °C, 65 95 respectively. These data help laboratory workers improve their protocols by handling the virus biosafety conditions.
Genome segmentation facilitates reassortment and rapid evolution of influenza A virus. However, complicates particle assembly as virions must contain all eight vRNA species to be infectious. Specific packaging signals exist that extend into the coding regions most if not segments, but these RNA motifs are poorly defined. We measured codon variability in a large dataset sequences identify areas low nucleotide sequence variation independent amino acid conservation each segment. Most clusters...
The culture supernatants of the emerging Middle East respiratory syndrome coronavirus ( MERS ‐CoV) were submitted to three temperatures over time and tested for infectivity by TCID 50 method on Vero E6 cells. At 56°C, almost 25 minutes necessary reduce initial titre 4 log 10 . Increasing temperature 65°C had a strong negative effect viral as virucidy dropped significantly 1 minute. On contrary, no significant decrease in was observed after 2 hours at 25°C. These data might be useful...
Abstract Supernatants of cells infected with SARS-CoV-2, nasopharyngeal and sera samples containing SARS-CoV-2 were submitted to heat inactivation for various periods time, ranging from 30 seconds 60 minutes. Our results showed that could be inactivated in less than minutes, 15 minutes 3 at 56°C, 65°C 95°C respectively. These data help laboratory workers improve their protocols handling the virus biosafety conditions.
Phi29 polymerase based amplification methods provides amplified DNA with minimal changes in sequence and relative abundance for many biomedical applications. RNA virus detection using microarrays, however, can present a challenge because phi29 cannot amplify nor small cDNA fragments (<2000 bases) obtained by reverse transcription of certain viral genomes. Therefore, ligation is necessary prior amplification. We adapted the QuantiTect Whole Transcriptome Kit (Qiagen) to our purposes...
Knowledge of influenza A virus survival in different environmental conditions is a key element for the implementation hygiene and personal protection measures by health authorities. As it dependent on isolates even within same subtype, we studied 2009 H1N1 pandemic (H1N1pdm) water non-porous surface. The H1N1pdm was subjected to various parameters over time tested infectivity. In water, at low medium salinity levels 4°C, survived least 200 days. Increasing temperature had strong negative...
Facioscapulohumeral muscular dystrophy (FSHD) is a dominant disease linked to contractions of the D4Z4 repeat array in 4q35. We have previously identified double homeobox gene (DUX4) within each unit that encodes transcription factor expressed FSHD but not control myoblasts. DUX4 and its target genes contribute global dysregulation expression observed FSHD. now characterized homologous DUX4c mapped 42 kb centromeric array. It 47-kDa protein with homeodomain identical divergent...
Different kinds of media spiked with monkeypox virus (MPXV) were subjected to heat inactivation at different temperatures for various periods time. The results showed that MPXV was inactivated in less than 5 min 70 °C and 15 60 °C, no difference between viruses from the West African Central clades. present findings could help laboratory workers manipulate optimal biosafety conditions improve their protocols.
Background: Human T-cell leukemia virus type 1 (HTLV-1), the causative agent of adult leukemia/lymphoma, shows intrapatient genetic variability. Although HTLV-1 can replicate via reverse transcription virion RNA to a double-stranded DNA provirus (the conventional manner for retroviruses), its predominant mode replication is clonal expansion (mitosis) infected cell. This achieved by viral oncoprotein Tax, which keeps CD4 T lymphocyte cycling. Because Tax also interferes with cellular repair...
Background Influenza A viruses have an envelope made of a lipid bilayer and two surface glycoproteins, the hemagglutinin neuraminidase. The structure virus is directly dependent on genetic makeup viral genome except glycosylation moieties composition bilayer. They both depend host cell are in direct contact with environment, such as air or water. Virus survival important for transmission from contaminated waters case wild aquatic birds humans. Objective objective this study was to check...
Background . Corynebacterium kroppenstedtii ( Ck ) was first described in 1998 from human sputum. Contrary to what is observed ethnic groups such as Maori, rarely isolated breast abscesses and granulomatous mastitis Caucasian women. Case Presentation We herein report a case of recurrent 46-year-old woman. Conclusion In the abscesses, even women, possible involvement should be investigated. The current lack investigations, probably due difficulty detect , may cause underestimation an aetiology.
The connectedness in Arctic regions between migratory waterbird populations originating from different continents and the potential for virus exchange at their shared breeding ground point to need explore largely unstudied circumpolar circulation of avian influenza viruses (AIV). We here report investigation AIV wild birds lakes a high area Northeast Greenland. No could be detected fecal, feather, water samples collected large flocks pink-footed geese Anser brachyrhynchus barnacle Branta...
ABSTRACT Human pathogenic retroviruses do not have common loci of integration. However, many factors, such as chromatin structure, transcriptional activity, DNA-protein interaction, CpG methylation, and nucleotide composition the target sequence, may influence integration site selection. These features been investigated by in vitro reactions or infection cell lines with recombinant retroviruses. Less is known about choice for vivo. The present study was conducted order to assess...
We report the whole-genome sequences of a monkeypox virus from skin lesion French patient and corresponding isolated viral strain. Both genomic were successfully obtained by applying shotgun metagenomics using Oxford Nanopore Technologies sequencing approach.
The transmission routes of Influenza A viruses (IAVs) submit virus particles to a wide range environmental conditions that affect their transmission. In water, temperature, salinity, and pH are important factors modulating viral persistence in strain-dependent manner, the driving IAV remain be described. We used an innovative method based on real-time cell system analysis quantify decay model. Thus, we identified hemagglutinin (HA) neuraminidase (NA) as main proteins by comparing...
Influenza A viruses (IAVs) are a major cause of infectious respiratory human diseases and their transmission is dependent upon the environment. However, role environmental factors on IAV survival outside host still raises many questions. In this study, we used lentiviral pseudotypes to study influence hemagglutinin protein in survival. High-titered cleaved influenza-based pseudoparticles, through use combination two proteases (HAT TMPRSS2) were produced. Pseudoparticles bearing proteins...
We report a new case of an asymptomatic carrier with deletion 3′ HTLV‐1 integration site. further investigated whether these deletions flanking sequences may explain the oligoclonal pattern replication, evidenced by inverse PCR (IPCR) analysis tumourous samples from patients adult T‐cell leukaemia (ATLL). 48 sites, derived DNA five ATLL were sequenced. One dominant sequence was obtained in four harbouring unique band after Southern‐blotting. In one sample, which harboured two signals...
Abstract Background A resequencing microarray called PathogenID v2.0 has been developed and used to explore various strategies of sequence selection for its design. The part dedicated influenza viruses was based on consensus sequences specific one gene generated from global alignments a large number virus available in databanks. Results For each HA (H1, H2, H3, H5, H7 H9) NA (N1, N2 N7) molecular type chosen be tested, 1 3 were computed tiled the microarray. total 12 samples different host...
Sequencing integration sites from ≥200 proviruses isolated infected individuals revealed that HTLV-1 is not random at the level of nucleotide sequence. The virus was found to integrate in A/T-rich regions with a weak consensus sequence positions within and without hexameric repeat generated during integration. These features were associated preference for near active or elements host chromosomes. However, about 6% be integrated into transcription units, suggesting some cells, may alter gene...
SARS-CoV-2 (Severe acute respiratory syndrome coronavirus 2), a virus causing severe disease in humans, emerged late 2019. This can spread via aerosols, fomites, contaminated hands or surfaces as for other coronaviruses. Studying their persistence under different environmental conditions represents key step better understanding the transmission. work aimed to present reproducible procedure collecting data of stability and inactivation kinetics from scientific literature. The aim was identify...