Little is known about the potential of restoration plantations to provide appropriate understory conditions support establishment seeds arriving from neighboring native forests. In this article, we investigated how seedling affected in sites different ages and assessed some environmental factors controlling ecological process. We first compared density richness tree seedlings among 10‐, 22‐, 55‐year‐old within Atlantic Forest region southeastern Brazil. Then, undertook a seed addition...

It is possible to determine the optimum time for permanence of vegetative propagules (mini-cuttings) inside a greenhouse rooting, and this value can be used optimize structure nursery. The aim study was dynamics adventitious rooting in mini-cuttings three clones Eucalyptus benthamii x dunnii. Sprouts H12, H19 H20 were collected from mini-stumps that planted gutters containing sand grown semi-hydroponic system. basal region immersed 2,000 mg L-1 indole-3-butyric acid (IBA) solution 10...

A micropropagação apresenta um enorme potencial de aplicação para a multiplicação genótipos espécies florestais brasileiras interesse.Os estudos relativos ao cultivo in vitro tais estão relacionados, principalmente, ausência resposta morfogênica às demais técnicas propagação bem como, conservação germoplasma.A via proliferação gemas axilares corresponde principal sistema utilizado selecionados, em razão da maior simplicidade comparar com organogênese e embriogênese somática.Entretanto, vista...

The difficulty and length of time required for seed germination mate (Ilex paraguariensis), as well the pressing need clonal multiplication improved genetic material, has resulted in several studies related to vegetative propagation an effort obtain rooted cuttings more quickly with better quality. Currently, biggest challenge is propagating rooting adult plants selected field without requiring clear cutting generate conditions basal induction juvenile sprouts. Therefore, objective this...

SummaryEucalyptus cloeziana is a species of great commercial interest due to the technical characteristics its wood. However, expansion areas planted limited by difficulties encountered with clonal propagation. The objective this study was develop method for cloning select adult trees micropropagation technique. Epicormic shoots were induced in branches and viable as explants vitro establishment. Shoots multiplied using Woody Plant Medium (WPM) culture supplemented 6-benzylaminopurine (BA)...

We addressed a major challenge in the vitro clonal propagation of Corymbia citriodora, Eucalyptus urophylla and E. benthamii by using an ex adventitious rooting strategy mini-incubator. Mini-incubators were placed four environments for rooting. A shade house with no fogging system greenhouse ventilation but environment had best performance terms rooting, root growth survival microcuttings. Daily recording temperature within each mini-incubator allowed verification negative correlations...

Eucalyptus has great importance in the forestry sector and many advances area of improvement have been achieved with advent biotechnological techniques. However, some promising genotypes still do not multiplication protocols cloning techniques, such as minicutting. The study aimed to evaluate IBA concentrations for survival, rooting vegetative vigor E. benthamii x dunnii minicuttings, determine maximum technical efficiency dose. Ministumps H12, H19 H20 clones were cultivated a clonal...

This study was designed to micropropagate E. benthamii x dunnii, by testing chlorine concentrations for explant asepsis, the optimal of benzylaminopurine (BAP) and naphthaleneacetic acid (NAA) bud proliferation, ratio between BAP gibberellic (GA3) in two nutrient media shoot elongation. Nodal segments from H12, H19 H20 clones were disinfected with 0.5, 1.0, 1.5 2.0% (v v-1) chlorine. Explants grown on ½MS medium supplemented (0, 0.25, 0.50, 0.75 1.00 mg L-1) NAA 0.025, 0.050, 0.075 0.100...

The aim of this study was to evaluate the influence irrigation method and substrate coverage on initial growth quality Myracrodruon urundeuva seedlings.We tested three different methods four types coverage.The response variables were total height, diameter at base stem, number leaves, Dickson index, survival.The use a water depth 12 mm once daily, administered in morning, best option for production 'aroeira' seedlings.Carbonized rice chaff as provided better standard seedlings, while sand...

O trabalho teve como objetivo avaliar a técnica de miniestaquia na clonagem Eucalyptus benthamii × dunnii ao longo das estações do ano quanto à sobrevivência e enraizamento miniestacas. As minicepas dos clones H12, H19 H20 foram manejadas durante 352 dias em minijardim clonal sob sistema semi-hidropônico leito areia com solução nutritiva fornecida por gotejamento, efetuando-se 27 coletas sucessivas brotações as quatro (primavera, verão, outono inverno). Mergulhou-se porção basal da...

Agrobacterium is the largest method employed to transform woody plants. The bacterium required introduce transgene into plant nuclear genome. After transferring T-DNA cell, bacteria affect growth negatively and have be eliminated from tissue culture medium through use of antibiotics. effect different antibiotics (timentin, cefotaxime carbenicillin) on in vitro shoot regeneration teak (Tectona grandis L. f.) was compared hypocotyl, mature cotyledon cotyledonary segments explants. Timentin...

Abstract Ontogenetic aging of tissues and the gradual decrease adventitious rooting are known challenges for clonal propagation woody species, hampering forestry programs. This study examined possible signatures tissue rejuvenation/reinvigoration in different propagated materials Eucalyptus microcorys by analyzing total protein profile, peroxidase activity, macro- micronutrient contents, mini cuttings. The analyses were performed on E. shoots which successfully obtained seminal grafting...

Neste trabalho foram testadas diferentes concentrações de cloro ativo (NaOCl) na assepsia explantes para o estabelecimento in vitro, bem como benzilaminopurina (BAP) e ácido naftalenoacético (ANA) a multiplicação alongamento Eucalyptus benthamii x dunnii. As minicepas fornecedoras propágulos introdução vitro conduzidas em minijardim clonal sob sistema semi-hidropônico. Segmentos nodais dos clones H12, H19 H20 desinfestados com 0,5; 1,0; 1,5; 2,0% (v/v) durante 10 min inoculados meio cultura...

O eucalipto e o genero florestal mais pesquisado em micropropagacao, entretanto, sao poucos os resultados efetivos obtidos com sua multiplicacao continua. A micropropagacao inclui a desinfestacao cultivo dos explantes meio nutritivo condicoes assepticas, propagulos sucessivos subcultivos enraizamento in vitro ou ex vitro. Como vantagens, possibilita: alto rendimento por planta matriz; obtencao de plantas isentas viroses; producao mudas qualquer epoca do ano; intensiva sadias, curto periodo...

We investigated the potential of RAPD-RFLP (Random Amplified Polymorphic DNA, Restriction Fragment Length Polymorphism) in detecting genetic relationships among bamboo genera and species.• RFLP profiles resolved acrylamide gels revealed high number markers, which accurately differentiated species genera, based on cophenetic correlation coefficients.• recommend for analyses diversity divergence varieties.