Transgenic plants over-expressing wasabi defensin gene were successfully produced in Phalaenopsis orchid by Agrobacterium-mediated transformation method. Embryogenic cell suspension culture of Wataboushi '#6.13' was infected with A. tumefaciens strain EHA101 carrying a plasmid containing and selectable marker nptII, hpt genes. Plantlets regenerated through somatic embryogenesis from the calli selected on hygromycin-containing medium. Transformation plantlets confirmed PCR analysis. Southern...

SummaryThe type and concentration of antibiotic used to eliminate Agrobacterium affected the efficiency bacterial elimination from, phytotoxicity Phalaenopsis cells. To Agrobacterium, high concentrations (500 mg l–1) cefotaxime carbenicillin were required, which also caused necrosis cells when added culture medium for 2 weeks. In contrast, meropenem successfully suppressed growth bacteria at low (5 had no phytotoxic effect. A reproducible genetic transformation method was established by...

Abstract The precious traits in the coloured grains of red, deep purple, or black has attracted more attention due to its health benefits. These benefits have been attributed part phytochemicals rice seed, including phenolic, flavonoid and anthocyanins which are known as source antioxidants. This study was aimed investigate content total anthocyanin from seventeen pigmented collected upland regions Toraja, Enrekang lowland Jeneponto, South Sulawesi. were dehusked using handheld husker (Kett...

This study compared hydro- and osmo-priming to determine period needed for seed priming indicator emerge its effect against water stress on germination percentage of some aerobic rice cultivars ( Oryza sativa L.). Experiments were arranged in completely randomized design using three PEG with four replications. Rice varieties (Inpago-8, IR64 Situ Bagendit) combined different concentrations. Drought was simulated by solutions distilled as control. Results indicated differentiations between...

Three series of plant growth regulators experiments in Chrysanthemum tissue culture, each arranged completely randomized design (CRD), were studied to formulate efficient regeneration system for developing cell engineering. In the first experiment, 100 mg callus cultured liquid Murashige and Skoog (MS) medium supplemented with different combinations NAA BAP concentrations treatments. After 30 days heaviest (1.55 g) was obtained (1.0 L-1) + (0.5 L-1). Consequently, shoot experiment kinds...

This study aims to obtain the genotype of local Toraja red rice M2 mutants that have potential be developed into early maturing varieties and high yields. Research carried out with a mass selection method consisting two irradiation treatments, namely: (1) Carbon ion dose 150 Gy (PL-C); (2) Argon 10 (PL-A) control as comparison. Selected strains seed population were planted in experimental plus 2 control, each row had 50 plants. Selection is done stages. The first stage, based on criteria...

Abstract. Al-Amanah H, Sjahril R, Haring F, Riadi M, Larekeng SH. 2021. Mapping distribution of Capsicum annum var. chinense in Tana Toraja and surrounding districts (Indonesia) based on fruit morphology. Biodiversitas 23: 982-991. Katokkon pepper (Capsicum chinense) is one the many genetic resources still cultivated small farms home gardens Toraja, North Enrekang Mamasa Indonesia. This study was conducted to identify diversity C. characteristics as morphological markers endemic area. The...

Local rice in general has several weaknesses among others, long life, high plant posture and low yield result. The character is a limiting factor that causes farmers interest to grow local rice. It feared this will cause the lack of cultivars as germplasm materials. Therefore, there an effort create diversity morphological characters, selection, especially related age harvest posture. One method through breeding mutation by irradiation using ion beam. objective research evaluate seed...

Abstract Tree, The Regency of Tana Toraja and North Toraja, are two regencies in the Province South Sulawesi where even though both not center rice crop development, but we can find many cultivated local genotypes. This study aims to determine relationship between genotypes found based on panicle grain characteristics correlation these characters. characters observed length, grains number per panicle, density, awn width, thickness, length width ratio 100 seeds weight. Results showed that 49...

Genetic Diversity of 22 Local Rice Accessions from North Toraja Based on Simple Sequence Repeats (SSR) MarkersABSTRACTOne way to explore the potential local rice is by characterization that could obtain genetic diversity plants. The aim this study was accession Toraja. Twenty-two accessions were characterized 30 SSR markers and using NTSYS pc 2.1 program analyze diversity. results showed twenty-six had been analyzed produced some alelles with a size between 106.75-311 bp, average number...

Background and Objective: The development of local aromatic rice cultivars has become a distinct segment market for plant breeders producers. This study aimed to identify the Genotypic Coefficient Variation (GCV), Phenotypic (PCV), heritability (h2 bs), close relationship between traits direct indirect effect lines based on among population observed. Materials Methods: field experiment was conducted in Enrekang Regency, South Sulawesi, Indonesia (650 m above sea level), from May-October,...

Abstract This study aims to identify the effectiveness of gamma-ray irradiation improve Indigofera seed viability. The employed Randomized Block Design (RBD) consisting 5 dosage (treatment) and 4 replication. treatment consisted Irradiation Dosage (A) 0Gy 50Gy; 100Gy;150Gy 200Gy observed parameter encompassed germination percentage, mortality plumule radicle length, index. results ANOVA indicated that irradiated seedling had a significant effect (P<0.05) on parameters percentage. was...

Abstract. Okasa AM, Sjahril R, Riadi M, Mahendradatta Sato T, Toriyama K, Ishii Hayashi Y, Abe T. 2021. Evaluation of Toraja (Indonesia) local aromatic rice mutant developed using heavy-ion beam irradiation. Biodiversitas 22: 3474-3481. The "Pare Bau" has a good grain quality and aroma. However, it some disadvantages, including late heading low yield for modern farming system. This study aims to evaluate select early as well high lines induced by Furthermore, dry seeds Pare Bau were...

Katokkon pepper plant is originated from Toraja, South Sulawesi, Indonesia. This endemic variety has unique hot and spicy characters with a distinctive bell aroma, aesthetic shape similar to paprika, but smaller size. research was conducted identify ploidy level morphology of colchicine induced at Laboratory Plant Reproduction Bioscience Biotechnology, Department Agronomy, Faculty Agriculture, Universitas Hasanuddin experimental field Agricultural Extension System Vocational High School...

Abstract This study aims to examine the good production characteristics and path analyses as well character correlation of highland F4 new plant type lines. The research was conducted in four paddy fields, namely Bittuang, Rantebua, Sesean, Tallunglipu sub-districts at altitudes 650, 850, 1400 1600 meters above sea level North Toraja Tana Regencies. took place from June December 2021 arranged randomized block design. genotypes tested were 30 lines six control varieties which repeated 3 times...

Genetic transformation mediated by Agrobacterium tumefaciens requires an efficient selection method for successful progress of transformation. This study aims to determine the concentration and kind antibiotics agents used during formulate standard protocol chrysanthemum in process propagating disease resistant Chrysanthemum EHA105 (pEKB-WD). The experiments were performed planting explants leaf cutting (5 mm diameter on NAA medium 2 mg L-1 BAP L-1) with addition Kanamycin: 25, 50, 100, 150...

Abstract Katokkon chili is a plant of the Solanaceae family which has unique characteristic fruit shape, spiciness level and also sweet taste. In addition, it diploid with 12 pairs chromosomes (2n=24). Quality improvement production katokkon can be done by doubling chromosomes. This research aimed to study effect several concentrations time immersion colchicine on buds shoots. Research activity was carried out soaking at (0.25%, 0.50%, 0.75%, 1.00%) for some times (1.5 hours, 3 hours 4.5...

Abstract. Limbongan YL, Driyunitha, Sjahril R, Riadi M, Jamaluddin I, Okasa AM, Panga NJ. 2021. Heritability and genetic advancement on agronomic characters of Toraja red rice x Inpari-4 white genotypes. Biodiversitas 22: 3446-3451. Determination diversity, heritability, advance specific cross-breed genotypes (Toraja Pare Lea’ Inpari-4) was done in Tallunglipu, North Toraja, South Sulawesi, Indonesia. The experiment designed using a randomized complete block design. Twenty-one days old...

The most important issue in food security Indonesia is limited land area which suitable for crops. While production already reached its highest potency. Eastern part of seems to have hidden answer the issues. Sago plant origin from Indonesia. Sulawesi known by unique semi-cultivated system production. This paper will describe transdisciplinary approach developing a model sago development based on local community. derived research findings related characteristic semi-cultivation as follows:...

This research was conducted to investigate harvest index and yield components of aerobic rice (Oryza sativa) under effect water, varieties seed priming in the tropical region. The experiment set split-split plot design. treatments were with poly ethylene glycol (PEG), treated (P1) untreated (P0) as sub-sub-plot, Three cultivars sub-plot consisted Inpago 8, new potential one (V1), IR64, susceptible cultivar (V2) Situbagendit, a normal (V3) sub-plot, three irrigation intervals namely irrigated...