A5011973588- Microbial bioremediation and biosurfactants
- Microbial Metabolic Engineering and Bioproduction
- Wastewater Treatment and Nitrogen Removal
- Biofuel production and bioconversion
- Microbial Community Ecology and Physiology
- Pesticide and Herbicide Environmental Studies
- Bacterial Genetics and Biotechnology
- Enzyme Catalysis and Immobilization
- Microbial metabolism and enzyme function
- Catalysis for Biomass Conversion
- Microbial Fuel Cells and Bioremediation
- Genomics and Phylogenetic Studies
- Membrane Separation Technologies
- Bacterial biofilms and quorum sensing
- Metal-Catalyzed Oxygenation Mechanisms
- Mine drainage and remediation techniques
- Metal Extraction and Bioleaching
- Anaerobic Digestion and Biogas Production
- Polyamine Metabolism and Applications
- Enzyme-mediated dye degradation
- Chromium effects and bioremediation
- Pharmaceutical and Antibiotic Environmental Impacts
- Membrane-based Ion Separation Techniques
- Fungal and yeast genetics research
- Radioactive element chemistry and processing
National Institute of Advanced Industrial Science and Technology
2015-2024
Management Research Institute
2017-2024
Institute for Environmental Management
2017-2024
The University of Tokyo
1996-2014
Biotechnology Research Center
2001-2008
Sphingomonas sp. strain P2, which is capable of utilizing phenanthrene as a sole carbon and energy source, was isolated from petroleum-contaminated soil in Thailand. Gas chromatography-mass spectrometry (1)H (13)C nuclear magnetic resonance analyses revealed two novel metabolites the degradation pathway. One identified 5,6-benzocoumarin, derived by dioxygenation at 1- 2-positions phenanthrene, other determined to be 1,5-dihydroxy-2-naphthoic acid. Other were 7, 8-benzocoumarin,...
ABSTRACT Glyceric acid (GA), an unfamiliar biotechnological product, is currently produced as a small by-product of dihydroxyacetone production from glycerol by Gluconobacter oxydans . We developed method for the efficient GA target compound new surplus applications in biodiesel and oleochemical industries. investigated ability 162 acetic bacterial strains to produce found that patterns productivity enantiomeric compositions obtained several differed significantly. The growth parameters two...
Deciphering unclear microbial interactions is key to improving biological wastewater treatment processes. Microbial predation and parasitism in ecosystems are unexplored survival strategies that have long been known recently attracted attention because these interspecies may contribute the reduction of excess sludge. Here, community profiling 600 activated sludge samples taken from six industrial one municipal processes (WWTPs) was conducted. To identify shared lineages WWTPs, constituents...
Batie et al. [Chemistry and Biochemistry of Flavoenzymes, 3, 543-556(1991)] proposed a classification system for ring-hydroxylating oxygenases in which the are grouped into three classes terms number constituent components nature th redox centers. But recent years, many have been newly identified characterized, found dificult to classify these classes. Typical examples carbazole 1,9a-dioxygenase 2-oxo-1,2-dihydroquinoline 8-monooxygenase, classified class III IB, respectively, from...
Two kinds of bacteria having different-structured angular dioxygenases-a dibenzofuran (DF)-utilizing bacterium, Terrabacter sp. strain DBF63, and a carbazole (CAR)-utilizing Pseudomonas CA10-were investigated for their ability to degrade some chlorinated dibenzofurans (CDFs) dibenzo-p-dioxins (CDDs) (or, together, CDF/Ds) using either wild-type strains or recombinant Escherichia coli strains. First, it was shown that CAR 1,9a-dioxygenase (CARDO) catalyzed dioxygenation all mono- triCDF/Ds in...
For acceleration of removing toxic metals from acid mine drainage (AMD), the effects hydraulic retention time (HRT) and pH on reactor performance microbial community structure in depth direction a laboratory-scale packed-bed bioreactor containing rice bran as waste organic material were investigated. The HRT was shortened stepwise 25 to 12 h, 8 6 5 h under neutral condition using AMD neutralized with limestone (pH 6.3), 20 7 3.0). Under condition, stably operated up HRT, which shorter than...
Abstract Although metagenomics researches have illuminated microbial diversity in numerous biospheres, understanding individual functions is yet difficult due to the complexity of ecosystems. To address this issue, we applied a metagenome-independent, de novo assembly–based metatranscriptomics complex microbiome, activated sludge, which has been used for wastewater treatment over century. Even though two bioreactors were operated under same conditions, their performances differed from each...
ABSTRACT The nucleotide sequences of the 27,939-bp-long upstream and 9,448-bp-long downstream regions carAaAaBaBbCAc (ORF7) Ad genes carbazole-degrading Pseudomonas sp. strain CA10 were determined. Thirty-two open reading frames (ORFs) identified, car gene cluster was consequently revealed to consist 10 ( carAaAaBaBbCAcAdDFE ) encoding enzymes for three-step conversion carbazole anthranilate degradation 2-hydroxypenta-2,4-dienoate. high identities (68 83%) with involved in...
Hybridization analysis showed that a newly isolated carbazole (CAR)-degrading bacterium Sphingomonas sp. strain KA1 did not possess the gene encoding terminal oxygenase component (carAa) of CAR 1,9a-dioxygenase at high homology (more than 90% identity) to another CAR-degrader, Pseudomonas resinovorans CA10. However, PCR experiments using primers for amplifying internal fragment carAa (810 bp CA10) product unexpected size (1100 bp) was amplified. Sequence revealed this DNA region contained...
Isolated from Pseudomonas resinovorans CA10, pCAR1 is a 199-kb plasmid that carries genes involved in the degradation of carbazole and dioxin. The nucleotide sequence has been determined previously. In this study, we characterized terms its replication, maintenance, conjugation. By constructing miniplasmids testing their establishment putida DS1, show replication due to repA gene upstream DNA region. putative oriV region could be separated P. was located within 345-bp between parW genes....
Abstract 1,4-Dioxane is one of the most common and persistent artificial pollutants in petrochemical industrial wastewaters chlorinated solvent groundwater plumes. Despite its possible biological treatment natural environments, identity dynamics microorganisms involved are largely unknown. Here, we identified active diverse 1,4-dioxane-degrading from activated sludge by high-sensitivity stable isotope probing rRNA. By rigorously analyzing 16S rRNA molecules RNA density fractions 13C-labeled...
Pseudozyma antarctica is a non-pathogenic phyllosphere yeast known as an excellent producer of mannosylerythritol lipids (MELs), multi-functional extracellular glycolipids, from vegetable oils. To clarify the genetic characteristics P. antarctica, we analyzed 18 Mb genome T-34. On basis KOG analysis, number genes (219 genes) categorized into lipid transport and metabolism classification in was one half times larger than that Saccharomyces cerevisiae (140 genes). The gene encoding ATP/citrate...
Biofilm formation on the filtration membrane and subsequent clogging of pores (called biofouling) is one most persistent problems in bioreactors for wastewater treatment reclamation. Here, we investigated structure microbiome fouling-related biofilms bioreactor using non-destructive confocal reflection microscopy high-throughput Illumina sequencing 16S rRNA genes. Direct indicated that thin were formed maintained regardless increasing transmembrane pressure, which a common indicator fouling,...