A5010278286- Blood properties and coagulation
- Protein purification and stability
- Viral Infectious Diseases and Gene Expression in Insects
- Transgenic Plants and Applications
- Skin and Cellular Biology Research
- RNA and protein synthesis mechanisms
- Monoclonal and Polyclonal Antibodies Research
Nagoya University
2021-2024
Human transglutaminase 1 (TG1) modulates skin development, while its involvement in diseases remains poorly understood, necessitating comprehensive exploration of substrate interactions. To study the profile TG1, an vitro selection system based on cDNA display technology was used to screen two peptide libraries with mutations at varying distance from reactive glutamine. Next-generation sequencing and bioinformatics analysis selected DNA pools revealed a detailed TG1 profile, indicating...
cDNA display is an in vitro technology based on a covalent linkage between protein and its corresponding mRNA/cDNA, widely used for the selection of proteins peptides from large libraries (1012) high throughput manner, their binding affinity. Here, we developed platform using next-generation sequencing (NGS) rapid comprehensive substrate profiling transglutaminase 2 (TG2), enzyme crosslinking glutamine lysine residues proteins. After screening control peptide library randomized at reactive...
Abstract Human transglutaminase 1 regulates skin development and is linked with several disease conditions yet fully unknown mechanisms. To uncover all of its roles in health disease, the understanding protein substrates their reactivity necessary. gain insight into substrate profile human 1, this study uses an vitro selection system based on cDNA display technology to screen two displayed peptide libraries differing number position mutated sites relative reactive glutamine, terms 1....
Abstract cDNA display is an in vitro technology based on a covalent linkage between protein and its corresponding mRNA/cDNA, where stable complex formed suitable for wide range of selection conditions. A great advantage the ability to handle enormous library size (10 12 ) microtube scale, matter days. To harness benefits, we aimed at developing platform which combines advantages with high-throughput accuracy next-generation sequencing (NGS) preferred substrate peptides transglutaminase 2...