Chronic kidney disease (CKD) is a severe disorder with an increasing incidence worldwide. An early detection may help to prevent its progression and minimize the risk of cardiovascular diseases as one major comorbidities. Recently, extracellular miRNAs like urinary exosomal became great interest non-invasive biomarkers which can be determined by RT-qPCR. But until now, there no consensus regarding normalization isolated from body fluids. The present study analyzed miR-16, miR-92a, miR-21,...

Thyroid adenomas are common benign human tumors with a high prevalence of about 5% the adult population even in iodine sufficient areas. Rearrangements chromosomal band 19q13.4 represent frequent clonal cytogenetic deviation these making them most non-random translocations epithelial at all. Two microRNA (miRNA) gene clusters i.e. C19MC and miR-371-3 located close proximity to breakpoint region rearrangements have been checked for possible up-regulation due genomic alteration. In 4/5 cell...

The high-mobility-group (HMG) protein gene HMGI-C is apparently involved in the genesis of a variety benign human solid tumors with rearrangements chromosomal region 12q14-15 affecting gene. So far, no expression has been found adult tissues, and data are available on primary malignant epithelial origin. Therefore, we analysed patterns 44 breast cancer samples 13 nonmalignant adjacent tissue by hemi-nested reverse transcriptase–polymerase chain reaction for expression. There was detectable...

The technique of RNA in situ hybridization to mouse embryo sections from different developmental stages was used perform a detailed analysis the expression pattern gene for architectural chromatin factor Hmgic. At early fetal development (day 9.5 post conceptionem), Hmgic is expressed at high rate throughout whole embryo. In second half development, becomes more restricted. Expression found mesenchymal derivatives, which differentiate into cartilage or muscle, epithelial cell layers lung,...

Epithelial tumors of the thyroid are cytogenetically well-investigated tumors. So far, main cytogenetic subgroups, characterized by trisomy 7 and rearrangements either 19q13 or 2p21, respectively, have been described. Recently, we able to describe involvement a novel gene called THADA in benign lesions with 2p21 rearrangements. Other fusion genes found RET/PTC PAX8/PPAR(gamma). The latter occurs follicular carcinomas t(2;3)(q13;p25). Here present molecular-cytogenetic investigations on...

Abstract Background Thyroid adenoma associated (THADA) has been identified as the target gene affected by chromosome 2p21 translocations in thyroid adenomas, but role of THADA is still elusive. The aim this study was to quantify expression normal tissues and hyper- neoplasias, using real-time PCR. Methods For analysis 18S rRNA assays were performed on 34 tissue samples, including thyroid, salivary gland, heart, endometrium, myometrium, lung, blood, adipose well 85 three adenomas with a...

In an attempt to identify the target gene of specific translocations involving chromosomal band 19q13 in benign follicular thyroid tumors, we have used two cell lines derived from tumors showing with breakpoints for fluorescence situ hybridization mapping studies cosmid and PAC clones located a 400-kbp region. The chromosome 19 abnormalities mapped within 140-kb segment covered by single clone. Sequencing part this clone allowed us establish cDNA sequence genomic structure candidate close...

Structural rearrangements involving the long arm of chromosome 19 characterize a cytogenetic subgroup benign thyroid tumors and constitute one most frequent specific abnormalities in epithelial tumors. Recently, we have been able to narrow down breakpoint region affected two cell lines covered by single PAC clone. Close that candidate gene has identified which tentatively referred as RITA (Rearranged In Thyroid Adenomas) now named ZNF331 according HUGO nomenclature. However, results had...

Chromosomal rearrangements of band 19q13.4 are frequent cytogenetic alterations in benign thyroid adenomas. Apparently, these lead to the upregulation genes encoding microRNAs two clusters mapping breakpoint region, i.e. miR-371-3 and C19MC. Since members both have been associated with neoplastic growth other tumor entities question arises whether or not their predisposes malignant transformation follicular cells thyroid. To address this we quantified expression miR-372 miR-520c-3p samples...

Fusion of the high-mobility group protein gene HMGIC to other genes due chromosomal rearrangements occurs in a variety human benign tumors. In contrast clearly derived from chromosomes, some ectopic sequences fused have been assigned chromosome 12 by CASH (chromosome assignment using somatic cell hybrids) analyses and thus can be assumed either result alternative splicing or represent true on 12. an attempt identify this exon, we sequenced entire intron 4. Four seven previously described...

The technique of RNA in situ hybridization to mouse embryo sections from different developmental stages was used perform a detailed analysis the expression pattern gene for architectural chromatin factor Hmgic. At early fetal development (day 9.5 post conceptionem), Hmgic is expressed at high rate throughout whole embryo. In second half development, becomes more restricted. Expression found mesenchymal derivatives, which differentiate into cartilage or muscle, epithelial cell layers lung,...

Structural rearrangements involving chromosome band 2p21 characterize a cytogenetic subgroup of benign thyroid tumors. To narrow down the breakpoints these aberrations, we established two cell lines from tumors showing translocations 2p21. These and one additional primary tumor were used for FISH-studies with 18 BAC clones. All mapped to cluster about 450 kb.

Structural rearrangements involving the long arm of chromosome 19 characterize a cytogenetic subgroup benign thyroid tumors. To localize breakpoint 19q13 aberrations, we have established three cell lines derived from tumors showing translocations in this region. We used these and four additional primary with abnormalities for fluorescence situ hybridization (FISH) mapping studies ten cosmid clones located between molecular markers POLD1 TNNT1. The breakpoints all mapped within 400 kb