A5104753205- Glycosylation and Glycoproteins Research
- Carbohydrate Chemistry and Synthesis
- Galectins and Cancer Biology
- Monoclonal and Polyclonal Antibodies Research
- RNA modifications and cancer
- Immunotherapy and Immune Responses
- Cancer-related gene regulation
- Plant Pathogens and Fungal Diseases
- Genetics and Neurodevelopmental Disorders
- Plant-Microbe Interactions and Immunity
- Mycorrhizal Fungi and Plant Interactions
- Chemokine receptors and signaling
University of Copenhagen
2019-2023
Significance Siglecs are immunomodulatory receptors that recognize sialic acid–carrying glycans with important functions in immunity. However, many of their natural ligands poorly defined. We generated a cell-based glycan array comprised library isogenic human cells displaying the greater complexity acids on diverse structures and glycoconjugates context cell surface. applied this to reveal fine binding specificities for sialoglycans, informed underlying required glycosyltransferase genes,...
Abstract Mucins are a large family of heavily O-glycosylated proteins that cover all mucosal surfaces and constitute the major macromolecules in most body fluids. primarily defined by their variable tandem repeat (TR) domains densely decorated with different O-glycan structures distinct patterns, these arguably convey much informational content mucins. Here, we develop cell-based platform for display production human TR O-glycodomains (~200 amino acids) tunable patterns O-glycans using...
Mucins and glycoproteins with mucin-like regions contain densely O-glycosylated domains often found in tandem repeat (TR) sequences. These O-glycodomains have traditionally been difficult to characterize because of their resistance proteolytic digestion, knowledge the precise positions O-glycans is particularly limited for these regions. Here, we took advantage a recently developed glycoengineered cell-based platform display production mucin TR reporters custom-designed O-glycosylation...
Abstract Small molecule inhibitors of glycosylation enzymes are valuable tools for dissecting glycan functions and potential drug candidates. Screening glycosyltransferases mainly performed by in vitro enzyme assays with difficulties moving candidates to cells animals. Here, we circumvent this employing a cell-based screening assay using glycoengineered expressing tailored reporter glycoproteins. We focused on GalNAc-type O-glycosylation selected the GalNAc-T11 isoenzyme that selectively...
Chemokine ligands and receptors regulate the directional migration of leukocytes. Post-translational modifications chemokine including O-glycosylation tyrosine sulfation have been reported to ligand binding resulting signaling. Through in silico analyses, we determined potential conserved sites on human murine CC receptors. Glyco-engineered CHO cell lines were used measure impact receptor CCR5, while mutation residues treatment with sodium chlorate performed determine effect sulfation....
Greenhouse cultivation of ornamentals is subjected to a high incidence soil-borne fungal pathogens. In Kalanchoe, these pathogens are responsible for root and stem rot, infection the vascular tissue. Well-known believed cause diseases. Yet, systematized survey lacking Kalanchoe produced commercially. Here, we studied occurrence associated with in Denmark production cuttings stock plants greenhouse facilities located Turkey Vietnam. Molecular identification complemented mycological...