A5057680996- Plasma Applications and Diagnostics
- Bacterial biofilms and quorum sensing
- Antimicrobial Peptides and Activities
- Antimicrobial agents and applications
- Infection Control and Ventilation
- Additive Manufacturing and 3D Printing Technologies
- Plasma Diagnostics and Applications
- Electrohydrodynamics and Fluid Dynamics
- Surface Modification and Superhydrophobicity
- Innovations in Concrete and Construction Materials
- Antifungal resistance and susceptibility
- Water Treatment and Disinfection
- Boron Compounds in Chemistry
- Legionella and Acanthamoeba research
- Inhalation and Respiratory Drug Delivery
- Orthopedic Infections and Treatments
- Nanoparticles: synthesis and applications
- bioluminescence and chemiluminescence research
- Children's Physical and Motor Development
- Aerosol Filtration and Electrostatic Precipitation
- Biochemical and Structural Characterization
- Nanoplatforms for cancer theranostics
- Wound Healing and Treatments
- Moringa oleifera research and applications
- Antimicrobial Resistance in Staphylococcus
University of Chemistry and Technology, Prague
2018-2025
Czech Academy of Sciences, Institute of Organic Chemistry and Biochemistry
2019-2020
A critical lack of personal protective equipment has occurred during the COVID-19 pandemic. Polylactic acid (PLA), a polyester made from renewable natural resources, can be exploited for 3D printing face masks using Fused Deposition Modelling technique. Since possible high porosity this material raised questions regarding its suitability protection against viruses, we have investigated microstructure scanning electron microscopy and aerosol generator photometer certified as test system...
Antibiotic-loaded polymethylmethacrylate-based bone cement has been implemented in orthopaedics to cope with implant-related infections associated the formation of bacterial biofilms. In context emerging resistance current antibiotics, we examined efficacy short antimicrobial peptide-loaded inhibiting adhesion and consequent biofilm on its surface.The ability α-helical peptides composed 12 amino acid residues prevent [methicillin-resistant Staphylococcus aureus (MRSA), epidermidis,...
Abstract The paper reports a low-cost handheld source of cold air plasma intended for biomedical applications that can be made by anyone (detailed technical information and step-by-step guide creating the NTP are provided). employs 1.4 W corona discharge in needle-to-cone electrode configuration is an extremely simple device, consisting basically two electrodes cheap power supply. To achieve best bactericidal effect, has been optimized on Escherichia coli . ability was further tested wide...
Due to the great increase in non-thermal plasma (NTP) bio-applications, especially thanks its antimicrobial properties, many types of NTP generating devices have been developed recently. However, a comparison these is difficult due differences setup studies testing them, e.g., species microorganisms used and sample preparations. In this study, we optimized robust reproducible standard protocol using Bacillus subtilis spores applied it compare seven different terms technical parameters...
Non-thermal plasma (NTP) is a well-known decontamination tool applicable for wide range of microorganisms and viruses. Since the recent COVID-19 pandemic highlighted need to decontaminate all daily used items, it highly desirable address applicability NTP, including its possible harmful effects. To best our knowledge, comprehensive characterization NTP effects on sensitive materials still lacking. We investigated potential damage common use inflicted by air atmospheric generated in...
The emergence of antibiotic resistance in opportunistic pathogens represents a huge problem, the solution for which may be treatment with combination multiple antimicrobial agents. Sodium salt cobalt bis-dicarbollide (COSAN.Na) is one very stable, low-toxic, amphiphilic boron-rich sandwich complex heteroboranes. This compound has wide range potential applications biological sciences due to its antitumor, anti-HIV-1, and antibiofilm activity. Our study confirmed ability COSAN.Na (in...
Candida albicans is the main causative agent of vulvovaginal candidiasis (VVC), a common mycosis in women, relapses which are difficult to manage due biofilm formation. This study aimed at developing novel non-toxic compounds active against spp. biofilms. We synthesised analogues natural antifungal peptides LL-III (LL-III/43) and HAL-2 (peptide VIII) originally isolated from bee venoms elucidated their structures by nuclear magnetic resonance spectroscopy. The haemolytic, cytotoxic,...
ABSTRACT Candida albicans has several virulence factors at its disposal, including yeast–hyphal transition associated with biofilm formation, phospholipases, proteases and hemolytic activity, all of which contribute to pathogenesis. We used synthetic derivative LL-III/43 antimicrobial peptide lasioglossin LL-III enhance effect azoles on attenuation C. factors. was able inhibit initial adhesion or formation strains 50 µM. Azoles, however, were ineffective this concentration. Using...
The increasing risk of antibiotic failure in the treatment Pseudomonas aeruginosa infections is largely related to production a wide range virulence factors. use non-thermal plasma (NTP) promising alternative antimicrobial treatment. Nevertheless, there still lack knowledge about effects NTP on factors production. We evaluated ability four NTP-affected P. strains re-form biofilm and produce Las-B elastase, proteases, lipases, haemolysins, gelatinase or pyocyanin. Highly strains-dependent...
Due to the emerging resistance of microorganisms and viruses conventional treatments, importance self-disinfecting materials is highly increasing. Such could be silver or its nanoparticles (AgNPs), both which have been studied for their antimicrobial effect. In this study, we compared biological effects AgNP coatings with without a plasma-polymerized hexamethyldisiloxane (ppHMDSO) protective film smooth copper under three ambient conditions that mimic potential medical use (dry wet...
Non-thermal plasma (NTP), generated by DC cometary discharge with a metallic grid at atmospheric pressure, was used to eradicate biofilm of Pseudomonas aeruginosa from titanium alloy Ti-6Al-4V carriers. The NTP exposure may reduce the ability signal molecules cause quorum sensing (QS) response in P. and as result, affect production virulence factors including formation. Two groups were compared, namely strains isolated environment (non-hospital) clinical isolates hospital environment. For...
The influence of a non‐thermal plasma (NTP) on the gram‐negative bacteria Escherichia coli and Pseudomonas aeruginosa , gram‐positive bacterium Staphylococcus epidermidis yeast Candida albicans grown agar or in biofilm form was compared. NTP produced by DC cometary discharge. biofilms were surface Ti‐6Al‐4V alloy often used manufacture prosthetic implants. exposure not only inhibited growth microorganisms cultures but also significantly suppressed viability prevented its re‐developed from...
A non-thermal plasma (NTP) is a promising tool against the development of bacterial, viral, and fungal diseases. The recently revealed microbial resistance to traditional drugs has increased interest in use NTPs. We have studied compared physical microbicidal properties two types NTP sources based on cometary discharge point-to-point electrode configuration corona point-to-ring configuration. electrical emission both discharges are reported. effect was tested three strains bacterium...