A5102890260- Neonatal Respiratory Health Research
- Inhalation and Respiratory Drug Delivery
- Lipid Membrane Structure and Behavior
- Surfactants and Colloidal Systems
- Hemoglobin structure and function
- Neuroscience of respiration and sleep
- Respiratory Support and Mechanisms
- Spectroscopy and Quantum Chemical Studies
- Vasculitis and related conditions
- Electrostatics and Colloid Interactions
- DNA and Nucleic Acid Chemistry
- Quantum, superfluid, helium dynamics
- Fatty Acid Research and Health
- Diagnosis and treatment of tuberculosis
- Protein Structure and Dynamics
- Proteins in Food Systems
- Surface Modification and Superhydrophobicity
- Mass Spectrometry Techniques and Applications
- Pickering emulsions and particle stabilization
- Wheat and Barley Genetics and Pathology
- Polymer Surface Interaction Studies
- Silicone and Siloxane Chemistry
- Cardiovascular and Diving-Related Complications
- Retirement, Disability, and Employment
- Hematological disorders and diagnostics
Oregon Health & Science University
2014-2024
University of Leicester
2022
University of Florida
2018-2020
Columbus Oncology and Hematology Associates
2018-2020
University of Portland
2013-2020
Yale University
2019
Calgary Laboratory Services
2017
University College Dublin
2008
Pulmonary and Critical Care Associates
2000-2007
Portland State University
2006
HALL, STEPHEN F.R.A.C.P.; BARR, W. M.D.; LIE, J. T. STANSON, A. KAZMIER, F. HUNDER, G. M.D. Author Information
The biophysical properties and physiologic effects of a series clinical exogenous pulmonary surfactants was compared to determine the importance hydrophobic apoproteins (SP-B C) as constituents these preparations. three studied, calf lung surfactant extract (CLSE), Survanta (Surfactant-TA), Exosurf, all contain dipalmitoyl phosphatidylcholine (DPPC) their major constituent. CLSE also 1 2% SP-B,C but Exosurf has additives hexadecanol tyloxapol instead enhance activity DPPC. In adsorption...
Surface pressure-area (*-A) isotherms during dynamic cycling were measured for films of dipalmitoyl phosphatidylcholine (DPPC) and column-separated fractions calf lung surfactant extract (CLSE).Emphasis was on defining the relative importance phospholipids (PPL), neutral lipids (N), hydrophobic proteins (SP) in facilitating respreading surface tension lowering within interfacial film itself.Solvent-spread a Wilhelmy balance studied at 23' 37OC over range rates initial concentrations giving...
This paper reports the detailed composition of molecular species phosphatidylcholines (PCs) in pulmonary surfactant from calves. PC isolated by thin-layer chromatography (TLC) was converted to benzoylated diradyl glyceride derivatives, which were separated TLC according linkage group. Quantification groups analysis total fatty acid content demonstrated that contained 97.2% diacyl, 2.4% alkyl-acyl, and 0.4% alkenyl-acyl compounds. The diacyl alkyl-acyl diglyceride derivatives into individual...
This paper examines two factors, shape deformation and surface viscosity, that affect measurements of tension lung surfactants with the oscillating bubble surfactometer. At lower tensions, compressed in this apparatus becomes deformed to an oblate ellipsoid cannot be analyzed rigorously using simplified (spherical) Laplace equation calculate from interfacial pressure drop. However, for small air bubbles present apparatus, analysis more general equations ellipsoids revolution shows effects...
We have shown previously that lateral compression of pulmonary surfactant monolayers initially induces separation two phases but these remix when the films become more dense (1). In studies reported here, we used fluorescence microscopy to examine role different constituents in remixing separated phases. Subfractions containing only purified phospholipids (PPL), proteins and (SP&PL), neutral (N&PL) were obtained by chromatographic components extracted calf (calf lung extract, CLSE)....
The inhibitory effects of oleic acid (OA) on the surface activity pulmonary surfactant were characterized by use oscillating bubble surfactometer, Wilhelmy balance, and excised rat lungs. Oscillating studies showed that OA prevented lavaged calf [0.5 mM phospholipid (PL)] from lowering tension below 15 mN/m at or above a molar ratio OA/PL = 0.5. In contrast to inhibition plasma proteins, increasing concentration did not eliminate acid, which occurred greater than 0.67 even concentrations 1.5...
This study reports the biochemical separation of hydrophobic constituents calf lung surfactant into separate fractions from which specific components are excluded.Gel permeation chromatography on LH-20 with acidified chloroformmethanol separated extract (CLSE) containing purified phospholipids (PPL), neutral lipids and (N&PL), or proteins (SP)-B -C together (SP&PL).Extraction acid to prevent phospholipid degradation after reduced recovery apoproteins in SP&PL.This fraction was therefore...