A5014581353- Adipose Tissue and Metabolism
- Adipokines, Inflammation, and Metabolic Diseases
- Autophagy in Disease and Therapy
- Cardiovascular Disease and Adiposity
- MicroRNA in disease regulation
- Immune cells in cancer
- RNA modifications and cancer
- Lipid metabolism and biosynthesis
- Single-cell and spatial transcriptomics
- Air Quality and Health Impacts
- RNA Research and Splicing
- Antioxidant Activity and Oxidative Stress
- Diet and metabolism studies
- Climate Change and Health Impacts
- Cardiovascular and exercise physiology
- Sleep and related disorders
- Inflammasome and immune disorders
- Calcium signaling and nucleotide metabolism
- Muscle metabolism and nutrition
- Biomarkers in Disease Mechanisms
- Energy and Environment Impacts
- Liver Disease and Transplantation
- Extracellular vesicles in disease
- Fibroblast Growth Factor Research
- Cancer-related molecular mechanisms research
Ben-Gurion University of the Negev
2016-2025
University of Haifa
2024
The Technological College of Beer Sheva
2015
Faculty of Public Health
2015
Human adipose depots are functionally distinct. Yet, recent single-nucleus RNA sequencing (snRNA-seq) analyses largely uncovered overlapping or similar cell-type landscapes. We hypothesized that adipocyte subtypes, differentiation trajectories and/or intercellular communication patterns could illuminate this depot similarity–difference gap. For this, we performed snRNA-seq of human subcutaneous visceral tissues (five ten samples, respectively). Of 27,665 nuclei in both depots, most were...
The inflammasome has been recently implicated in obesity-associated dys-metabolism. However, of its products, the specific role IL-1β was clinically demonstrated to mediate only pancreatic beta-cell demise, and mice mainly intra-hepatic manifestations obesity. Yet, it remains largely unknown if IL-1β, a cytokine believed function locally, could regulate dysfunctional inter-organ crosstalk Here we show that High-fat-fed (HFF) exhibited preferential increase portal compared systemic blood....
Autophagy genes' expression is upregulated in visceral fat human obesity, associating with obesity-related cardio-metabolic risk. E2F1 (E2F transcription factor 1) was shown cancer cells to transcriptionally regulate autophagy. We hypothesize that regulates adipocyte autophagy endocrine/metabolic dysfunction, thereby, representing non-cell-cycle function of this factor. protein (N=69) and mRNA (N=437) were elevated obese humans, correlating increased ATG5 (autophagy-related 5), MAP1LC3B/LC3B...
In our previous research the antihypertensive properties of lycopene-containing tomato oleoresin have been revealed. The present study was aimed to assess if interferes in inflammatory signalling endothelial cells, imitating reduction processes vessel wall and this way propose mechanism for blood pressure by oleoresin.A wide number functional markers were investigated two cultured cell models [EA.hy926 human umbilical vein (HUVEC)], exposed carotenoids lycopene lutein. All significantly...
Increasing energy expenditure via induction of adipose tissue browning has become an appealing strategy to treat obesity and associated metabolic complications. Herein, we identify adipocyte-expressed apoptosis signal-regulating kinase 1 (ASK1) as regulator browning. High fat diet-fed adipocyte-specific ASK1 knockout mice reveal increased UCP1 protein levels in inguinal concomitant with elevated expenditure, reduced ameliorated glucose tolerance compared control littermates. In addition,...
Protein S-nitrosylation is a reversible protein modification implicated in both physiological and pathophysiological regulation of function. In obesity, skeletal muscle insulin resistance associated with increased insulin-signaling proteins. However, whether adipose tissue similarly affected obesity and, if so, what are the causes functional consequences this unknown. Total was intra-abdominal obese humans high fat-fed or leptin-deficient ob/ob mice. Both receptor β-subunit Akt were...
Obesity variably disrupts human health, but molecular-based patients' health-risk stratification is limited. Adipose tissue (AT) stresses may link obesity with metabolic dysfunction, how they signal in humans remains poorly-characterized. We hypothesized that a transcriptional AT stress-signaling cascade involving E2F1 and ASK1 (MAP3K5) molecularly defines high-risk obese subtype.ASK1 expression biopsies was determined by real-time PCR analysis, chromatin immunoprecipitation (ChIP) adopted...
Background Visceral adipose tissue foam cells are increased in human obesity, and were implicated dysfunction cardio-metabolic risk. In the circulation, non-classical monocytes (NCM) elevated obesity associate with atherosclerosis type 2 diabetes. We hypothesized that circulating NCM correlate and/or functionally linked to visceral potentially providing an approach estimate status non-surgical obese patient. Methods preformed ex-vivo functional studies utilizing sorted monocyte subclasses...
Deconvolution algorithms mostly rely on single-cell RNA-sequencing (scRNA-seq) data applied onto bulk (bulk RNA-seq) to estimate tissues' cell-type composition, with performance accuracy validated deposited databases. Adipose cellular composition is highly variable, and adipocytes can only be captured by single-nucleus (snRNA-seq). Here we report the development of sNucConv, a Scaden deep-learning-based deconvolution tool, trained using 5 hSAT 7 hVAT snRNA-seq-based corrected (i)...
Background: Metabolic dysfunction–associated steatotic liver disease (MASLD) is associated with an unhealthy lifestyle. However, there limited prospective evidence regarding the association between combined lifestyle factors and MASLD. This study aims to test of a combination components, expressed as healthy index (HLI), unhealthful eating behavior habits MASLD, insulin resistance (IR), fibrosis, metabolic steatohepatitis. Methods: A cohort was conducted among participants hepatic screening...
Objective: Obesity-induced-dysglycemia and hypothalamic-microgliosis coincide, but whether they remain linked upon obesity reversal, what is the effect of age, unclear. Here we hypothesized that rapid normalization dysglycemia obesity-reversal remains to microgliosis resolution, differs between young mid-aged mice. Methods: Young (7w) (1y) mice were fed normal chow (NC) or high-fat diet (HFD,8w), then switched NC (Rev,2w). Results: Compared mice, NC-fed heavier weight-stable, gained weight...
<title>Abstract</title> Purpose <italic>Helicobacter pylori</italic> (HP) infects up to half of the global population, though its prevalence is declining due improved hygiene and eradication therapies. In contrast, eosinophilic esophagitis (EoE) incidence rising, potentially linked hypothesis. Studies suggest an inverse relationship between HP EoE, but esophageal eosinophilia (EE) can arise from various causes, including infections. The aim this study was examine association EE effect...
Elevated expression of E2F1 in adipocyte fraction human visceral adipose tissue (hVAT) associates with a poor cardiometabolic profile. We hypothesized that beyond directly activating autophagy and MAP3K5 (ASK)–MAP kinase signaling, governs distinct transcriptome contributes to metabolic dysfunction obesity. performed RNA sequencing hVAT samples from age-, sex-, BMI-matched patients, all obese, whose protein was either high (E2F1high) or low (E2F1low). Tumor necrosis factor superfamily...
CTRP6 (C1qTNF6), a member of adiponectin gene family, regulates inflammation and metabolism in established obesity. Here, short-term high-fat feeding mice is shown to increase adipose tissue expression before changes the classical inflammatory genes occur. Conversely, decreases early course obesity reversal. Gain- loss-of-function models suggest as positive regulator cascades, negative adipogenesis expansion.
Obesity, a condition most commonly associated with hyper-leptinemia, is also characterized by increased expression of autophagy genes and likely autophagic activity in human adipose tissue (AT). Indeed, circulating leptin levels were previously shown to positively associate the such as Autophagy related gene-5 (ATG5). Here we hypothesized that acts an autocrine-paracrine manner increase two major AT cell populations, adipocytes macrophages. We followed dynamics autophagosomes following acute...
The identification of human obesity sub-types may improve the clinical management patients with and uncover previously unrecognized mechanisms. Here, we hypothesized that adipose tissue (AT) mast cells (MC) estimation could be a mark for sub-phenotyping beyond current clinical-based stratifications, both cross-sectionally prospectively. We estimated MC accumulation using immunohistochemistry gene expression in abdominal visceral AT (VAT) subcutaneous (SAT) cohort 65 persons who underwent...
Obesity promotes the biogenesis of adipose tissue (AT) foam cells (FC), which contribute to AT insulin resistance. Autophagy, an evolutionarily-conserved house-keeping process, was implicated in cellular lipid handling by either feeding and/or degrading lipid-droplets (LDs). We hypothesized that beyond phagocytosis dead adipocytes, AT-FC is supported microenvironment regulating autophagy. Non-polarized ("M0") RAW264.7 macrophages exposed conditioned media (AT-CM) exhibited a markedly...
Circulating miRNAs are increasingly being considered as biomarkers in various medical contexts, but the value of analyzing isomiRs (isoforms canonical miRNA sequences) has not frequently been assessed. Here we hypothesize that an in-depth analysis full circulating landscape could identify specific stronger biomarkers, compared to their corresponding miRNA, for identifying increased CV risk patients with non-alcoholic fatty liver disease (NAFLD)—a clinical unmet need. Plasma were sequenced...
Chromatin immunoprecipitation (ChIP) has become a central method when studying in vivo protein-DNA interactions, with the major challenge being hope to capture "authentic" interactions. While ChIP protocols have been optimized for use specific cell types and tissues including adipose tissue-derived cells, working protocol addressing challenges imposed by fresh whole human tissue not described. Utilizing paired omental subcutaneous obtained during elective abdominal surgeries, we carefully...