A5100376275- RNA modifications and cancer
- CRISPR and Genetic Engineering
- Neurogenetic and Muscular Disorders Research
- RNA Interference and Gene Delivery
- Thyroid Disorders and Treatments
- Virus-based gene therapy research
- Viral Infections and Immunology Research
- Hemoglobinopathies and Related Disorders
- Congenital heart defects research
- Animal Genetics and Reproduction
- Fetal and Pediatric Neurological Disorders
- Acute Myeloid Leukemia Research
- Prenatal Screening and Diagnostics
- Endometrial and Cervical Cancer Treatments
- Cancer-related molecular mechanisms research
- Cervical Cancer and HPV Research
- Ferroptosis and cancer prognosis
- Genetics, Bioinformatics, and Biomedical Research
- CAR-T cell therapy research
- Transplantation: Methods and Outcomes
- Endometriosis Research and Treatment
- Cancer, Lipids, and Metabolism
- Cardiac, Anesthesia and Surgical Outcomes
- Advanced biosensing and bioanalysis techniques
- Liver Disease Diagnosis and Treatment
Central South University
2016-2023
Boston University
2023
Yunnan Maternal and Child Health
2023
China Pharmaceutical University
2021
The JAK2 V617F mutation is a major diagnostic, therapeutic, and monitoring molecular target of Philadelphia-negative myeloproliferative neoplasms (MPNs). To date, numerous methods detecting the have been reported, but there no gold-standard diagnostic method for clinical applications. Here, we developed validated an efficient Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR associated protein 12a (Cas12a)-based assay to detect mutation. Our results showed that...
Spinal muscular atrophy (SMA) is characterized by severe lethality and irreversible progression. Early diagnosis of SMA more practical significance with the emergence effective therapy. However, existing techniques to identify patients rely on cumbersome instruments, hindering their accessibility application. An SMA-Cas12a-strip assay was developed integration Cas12a-based nucleic acid detection, isothermal amplification, lateral flow strip. The analytical performance assessed clinical...
Primary congenital hypothyroidism (CH) is a common neonatal endocrine disorder characterized by elevated concentrations of thyroid stimulating hormone (TSH) and low free thyroxine (FT4). PAX8 NKX2-1 are important transcription factors involved in development. In this study, we detected three novel variants (c.149A > C c.329G A) (c.706A G) whole exome sequencing (WES) unrelated CH patients with variable phenotypes. The results Western blot immunofluorescence analysis showed that the had no...
Jianyan Pan, Ph.D., Chunhua Zhang, Yanling Teng, M.S., Sijing Zeng, Siyi Chen, Desheng Liang, M.D., Zhuo Li, and Lingqian Wu, Ph.D.. Ann Lab Med 2021;41:101-7. https://doi.org/10.3343/alm.2021.41.1.101
Clustered regularly interspaced short palindromic repeat (CRISPR)-based biosensors have been developed to facilitate the rapid and sensitive detection of nucleic acids. However, most approaches using CRISPR-based disadvantages associated with limitations CRISPR RNA (crRNA), protospacer adjacent motif (PAM) or flanking sequence restriction, single channel detection, difficulty in quantitative resulting only some target sites being detected qualitatively. Here, we aimed develop a barcode-based...
Spinal muscular atrophy (SMA) is the main genetic cause of infant death. In >95% patients with SMA, disease caused by a single hotspot pathogenic mutation: homozygous deletion exon 7 survival motor neuron 1 gene (SMN1). Recently, clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR associated protein (Cas)-based assays have been developed as promising new option for nucleic acid detection. Here, we Cas14a1-based assay combined asymmetric PCR to establish method detection...
To explore the feasibility of non-invasive prenatal testing (NIPT) for detecting fetal chromosomal copy number variants (CNV).
Background: To explore the curative effects of laparosopic panhysterectomies and summarize perioperative care experience.Methods: Forty cases hysterectomy, twenty laparoscopic hysterectomies abdominal were selected for a controlled analysis treatment efficacies different nursing measures.Results: Laparoscope surgery durations longer than durations. Laparoscopic had lesser bleeding, quicker postoperative ambulation times, shorter intestinal bowel function recovery lower patient stress, better...
Objective To explore the value of CA125 combined with endometrial polyp in diagnosis early endometriosis. Methods The data 1 049 cases reason-unknown infertility diagnosed and treated at Foshan First People's Hospital from Jan. 2010 to Dec. 2015. were retrospectively analyzed. Results The raise serum had a sensitivity 77.69% prediction endometriosis, specificity 31.45%. Endometrial 84.21% 33.84%. together joint 93.27% 31.75%. By comparing CA125(positive) polyp, we realized that...
Abstract Background: Breast cancer stem cells (BCSCs) are positively correlated with metastasis, chemoresistance and recurrence of breast cancer. Methods: The stemness were analyzed by qPCR western blot, flow cytometry, cell spheroid formation assay, tumor xenograft model. viability was performed MTT. transcriptome analysis used to evaluate the influence these three compounds. migration wound-healing transwell invasion analysis, metastasis iron concentration fluorescence microscopy analysis....