A5088975843- Plant pathogens and resistance mechanisms
- Genetics and Plant Breeding
- Genetic Mapping and Diversity in Plants and Animals
- Legume Nitrogen Fixing Symbiosis
- Plant Pathogenic Bacteria Studies
- Soybean genetics and cultivation
- Plant Virus Research Studies
- Plant-Microbe Interactions and Immunity
- Plant Reproductive Biology
- Agricultural pest management studies
- Photosynthetic Processes and Mechanisms
- Genetic and Environmental Crop Studies
- Postharvest Quality and Shelf Life Management
- Plant Molecular Biology Research
- Plant responses to elevated CO2
- Plant Stress Responses and Tolerance
- Plant Disease Resistance and Genetics
- Plant responses to water stress
- Banana Cultivation and Research
- Chromosomal and Genetic Variations
- Genomics and Phylogenetic Studies
- Plant Surface Properties and Treatments
- Greenhouse Technology and Climate Control
- Plant Genetic and Mutation Studies
- Nanocomposite Films for Food Packaging
University of Florida
2016-2025
U.S. Horticultural Research Laboratory
2016
Twin Cities Orthopedics
1995
University of Minnesota
1995
U.S. Vegetable Laboratory
1991
New Mexico State University
1986
Carnegie Institution for Science
1983-1984
University of California, Davis
1979-1983
Carnegie Department of Plant Biology
1983
Abstract A seed and flower color marker (P), nine protein, isozyme 224 restriction fragment length polymorphism loci were used to construct a linkage map of the common bean, Phaseolus vulgaris L. (n = 11). The mapping population consisted backcross progeny between Mesoamerican breeding line 'XR-235-1-1' Andean cultivar 'Calima'; former was as recurrent parent. bean PstI genomic library enriched for single copy sequences (95%) source DNA probes. Sixty percent probes tested detected...
Productivity of rice, world's most important cereal is threatened by high temperature stress, intensified climate change. Development heat stress-tolerant varieties one the best strategies to maintain its productivity. However, stress tolerance a multigenic trait and candidate genes are poorly known. Therefore, we aimed identify quantitative loci (QTL) for vegetative stage in rice corresponding genes. We used genotyping-by-sequencing generate single nucleotide polymorphic (SNP) markers...
Realizing the potential of agricultural genomics into practical applications requires quantitative predictions for complex traits and different genotypes environmental conditions. The objective this study was to develop test a procedure prediction phenotypes as function environment specific genetic loci in soybean [ Glycine max (L.) Merrill]. We combined ecophysiological model CROPGRO‐Soybean with linear models that predict cultivar‐specific parameters functions E loci. involved three steps:...
A set of 219 DNA clones derived from mungbean (Vigna radiata), cowpea (V. unguiculata), common bean (Phaseolus vulgaris), and soybean (Glycine max) were used to generate comparative linkage maps among mungbean, bean, soybean. The allowed an assessment conservation collinearity the three genomes. Mungbean both subtribe Phaseolinae, exhibited a high degree preservation marker order. Most groups consisted only one or two blocks (and vice versa). situation was significantly different with...
The maize mutation sh2-7527 was isolated in a conventional breeding program the 1970s. Although mutant contains foreign sequences within gene, is not attributable to an interchromosomal exchange or chromosomal inversion. Hence, caused by insertion. Sequences at two Sh2 borders have been scrambled mutated, suggesting that insertion catastrophic reshuffling of genome. large, least 12 kb, and highly repetitive maize. As judged hybridization, sorghum only one few copies element, whereas no...
Cytoplasmic male sterility in the common bean plant is associated with a dominant mitochondrial mutation designated pvs-orf239 (for Phaseolus vulgaris sequence open reading frame 239). The transcribed both vegetative and reproductive tissues, but translation product, ORF239, present only tissues. We evidence to support model of post-translational regulation ORF239 expression based on following observations. In organello experiments using purified mitochondria from young seedlings...
Abstract The I locus of the common bean, Phaseolus vulgaris, controls development four different phenotypes in response to inoculation with Bean mosaic virus, necrosis several other related potyviruses, and one comovirus. We have generated a high-resolution linkage map around this aligned it physical constructed BAC clones. These clones were obtained from library cultivar “Sprite,” which carries dominant allele at locus. identified large cluster TIR–NBS–LRR sequences associated within locus,...
Maternal control of seed size in the common bean provides an opportunity to study genotype-independent weight effects on early seedling growth and development. We set out test hypothesis that heterotrophic seedlings is determined by both relative amount cotyledon storage reserves genotype seedling, provided hybrid could be fully expressed seedlings. The was tested via comparison phenotypes small-seeded (wild, ~0.10 g) large-seeded (landrace, ~0.55 parents their reciprocal F1 hybrids....
A number of physiologically based tomato crop models have been developed for use in studying greenhouseenvironment control. However, these may hundreds state variables and thus cannot be used with optimalcontrol methods to determine how operate greenhouse environment control equipment over time maximize profit. Theobjectives this research were develop a dynamic growth model minimum andto its applicability across different growing conditions. Our overall approach was simplify an existing...
Primers based on a conserved nucleotide binding site (NBS) found in several cloned plant disease resistance genes were used to amplify DNA fragments from the genome of common bean (Phaseolus vulgaris). Cloning and sequence analysis these uncovered eight unique classes disease-resistance related sequences. All contained kinase 2 motif, five 3a motif. Gene expression was noted for A clone SB3 class mapped 17.8 cM Ur-6 gene that confers races rust pathogen Uromyces appendiculatus. Linkage...
Strains of tomato race 3 (T3) Xanthomonas campestris pv. vesicatoria elicit a hypersensitive response (HR) in leaves Lycopersicon pennellii LA716. Genetic segregation the resistance exhibited ratios near 3:1 F2 populations, which confirmed that single dominant gene controlled inheritance this trait. With aid collection introgression lines, restriction fragment length polymorphism, and cleaved amplified polymorphic sequence markers, locus was located on chromosome between TG599 TG134. An...
Xanthomonas campestris pv. vesicatoria causes bacterial spot, one of the most serious diseases tomatoes. The lycopersicon esculentum accession 'Hawaii 7998' is only reliable source resistance to race 1 strains pathogen. This associated with a hypersensitive reaction controlled by multiple nondominant genes. inoculated area becomes fully necrotic 24 hr after inoculation in 7998,' whereas full necrosis observed 5 and 4 days susceptible species L. pennellii (LA 716) their F1, respectively. An...
ABSTRACT DNA restriction fragments containing sequences homologous to the ribosomal RNA (45s), major chlorophyll a/b binding polypeptide (CAB) and small subunit of ribulose bisphosphate carboxylase (RBCS) genes have been localized mapped in tomato nuclear genome by linkage analysis. Ribosomal map a single locus, R45s, which resides terminal position on short arm chromosome 2 corresponds Nucleolar Organizer Region. The size 45s repeating unit is estimated be approximately 9 kb Lycopersicon...
Common bean (Phaseolus vulgaris), a staple food in Latin America and Africa, serves as vital source of energy, protein, essential minerals for millions people. However, genomics knowledge that breeders could leverage improvement this crop is scarce. We have developed validated comparative approach to predict conserved transcription factor binding sites (TFBS) common studied gene regulatory networks. analyzed promoter regions identified TFBS 12,631 genes with an average 6 motifs per gene....
Every phenotypic trait can be viewed as a "system" in which group of interconnected components function synergistically to yield unified whole. Once system's and their interactions have been delineated according biological principles, we manipulate engineer functionally relevant produce desirable system phenotype.We describe conceptual framework for mapping quantitative loci (QTLs) that control complex traits by treating formation dynamic system. This framework, called systems mapping,...
High density genetic maps are a reliable tool for dissection of complex plant traits. Mapping resolution is often hampered by the variable crossover and non-crossover events occurring across genome, with pericentromeric regions (pCENR) showing highly suppressed recombination rates. The efficiency linkage mapping can further be improved characterizing understanding distribution recombinational activity along individual chromosomes. In order to evaluate genome wide rate in common beans...
The next generation of gene-based crop models offers the potential predicting vegetative and reproductive development based on genotype weather data as inputs. Here, we illustrate an approach for developing a dynamic modular model to simulate changes in main stem node numbers, time first anthesis, final number common bean (Phaseolus vulgaris L.). In modules, these characteristics are functions relevant genes (quantitative trait loci (QTL)), environment (E), QTL × E interactions. was from 187...