James Muleme

ORCID: 0000-0001-8967-7031
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Research Areas
  • Child Nutrition and Water Access
  • Antibiotic Resistance in Bacteria
  • Pharmaceutical and Antibiotic Environmental Impacts
  • Animal Disease Management and Epidemiology
  • Viral Infections and Vectors
  • Viral gastroenteritis research and epidemiology
  • Brucella: diagnosis, epidemiology, treatment
  • Zoonotic diseases and public health
  • Antibiotic Use and Resistance
  • COVID-19 epidemiological studies
  • Food Security and Health in Diverse Populations
  • Animal Diversity and Health Studies
  • Viral Infections and Outbreaks Research
  • Hepatitis B Virus Studies
  • Vaccine Coverage and Hesitancy
  • Vibrio bacteria research studies
  • Parasitic Infections and Diagnostics
  • Leptospirosis research and findings
  • Rabies epidemiology and control
  • Tuberculosis Research and Epidemiology
  • Agricultural Innovations and Practices
  • Infection Control in Healthcare
  • Global Maternal and Child Health
  • Pediatric health and respiratory diseases
  • Amoebic Infections and Treatments

Makerere University
2017-2025

Knowledge, attitude, and practice (KAP) studies guide the implementation of public health interventions (PHIs), they are important tools for political persuasion. The design PHIs assumes a linear KAP relationship, i.e., an awareness campaign results in desirable societal behavioral change. However, there is no robust framework testing this relationship before after PHIs. Here, we use qualitative quantitative data on pesticide usage to test identify associated context specific factors as well...

10.3389/fpubh.2017.00318 article EN cc-by Frontiers in Public Health 2017-12-08

Brucellosis is a worldwide recognized bacterial zoonotic disease. There currently no information on bovine brucellosis sero-prevalence in South Sudan regardless of the economic, social and public health impact populations. Therefore, for first time 33 years, we report cattle their herders. Furthermore, characterize drivers associated with disease at human-animal interface Bahr el Ghazal region, Sudan.A total 893 87 animal human sera respectively were examined between December 2015 May 2016....

10.1371/journal.pntd.0006456 article EN cc-by PLoS neglected tropical diseases 2018-06-20

Abstract Background More than 70% of new, emerging, and reemerging infectious diseases are from animal origin. Human interaction with bats has been associated as a driver for various fetal zoonoses, including numerous viral bat-origin. A lot serological evidence gathered around human-bat interaction, yet very little is known regarding the underlying risk factors at community level. This study was aimed understanding interactions among communities in Bundibugyo District Uganda. Methods...

10.1101/2025.01.19.25320814 preprint EN cc-by medRxiv (Cold Spring Harbor Laboratory) 2025-01-20

Background Brucellosis is a zoonotic disease with significant public health and economic effects on societies. In Uganda, brucellosis endemic primary contributor in livestock productivity losses. This more worrisome for populations the cattle corridor high reliance milk nutritional value symbol social relations identity. The community’s construction may affect comprehension of hence leading to exposure increased vulnerability transmission. Despite brucellosis’ prevalence corridor, little...

10.1371/journal.pone.0320364 article EN cc-by PLoS ONE 2025-04-24

Aim An evaluation exercise was carried out to assess the performance of Community Animal Health Workers (CAHWs) in delivery animal health care services Karamoja region, identify capacity gaps and recommend remedial measures. Materials & methods Participatory were used design data collection tools. Questionnaires administered 204 CAHWs, 215 farmers 7 District Veterinary Officers (DVOs) collect quantitative data. Seven DVOs 1 Non Government Organization (NGO) representative interviewed as key...

10.1371/journal.pone.0179110 article EN cc-by PLoS ONE 2017-06-08

Background Antibiotics are increasingly becoming ineffective as antimicrobial resistance (AMR) continues to develop and spread globally—leading more difficult treat infections. Countries such Uganda still challenged with implementation of AMR related strategies due data paucity. This includes a lack on the prevailing knowledge awareness antibiotic use among farming communities, both commercial subsistence, which instrumental in targeted interventions. The aim our study was assess knowledge,...

10.1371/journal.pone.0284822 article EN cc-by PLoS ONE 2023-06-02

Extended-spectrum beta-lactamase-producing Escherichia coli (ESBL-Ec) represents a significant global public health concern. The epidemiology of ESBL-Ec in Uganda is not well understood although it harbored by humans, animals, and the environment. This study explains using one approach selected farming households Wakiso district, Uganda.Environmental, human, animal samples were collected from 104 households. Additional data obtained observation checklists through interviews with household...

10.1371/journal.pgph.0001344 article EN cc-by PLOS Global Public Health 2023-06-13

Rabies is a zoonotic disease that mainly transmitted to humans through dog bites. It remains major public health threat in many Asian and African countries, including Uganda. The main objective of this study was investigate awareness, knowledge, perceptions communities toward human related rabies prevention, as well management practices within Masaka district, central Data collection involved nine key informant interviews (KIIs) six focus group discussions (FGDs). Methods used during...

10.3389/fvets.2022.863526 article EN cc-by Frontiers in Veterinary Science 2022-06-13

Background: In this study, we focused on three zoonotic brucellosis risk groups; abattoir workers, febrile cases at Wau hospital and cattle herders, in South Sudan. Competitive c-ELISA was used to detect anti-Brucella antibodies 725 individuals between December 2015 May 2016. addition, questionnaire metadata, focus group discussions key informant interviews were characterize the epidemiology of region. Results: Overall, estimate 27.2 % (95% CI=23.9-30.6) sero-prevalence; 32.1 CI=26.2-38.4),...

10.3389/fpubh.2019.00156 article EN cc-by Frontiers in Public Health 2019-06-26

Our study aimed at assessing the determinants of hand hygiene and utilisation bathing facilities in healthcare (HCFs) greater Kampala metropolitan area, Uganda. Results indicate that 19.9% respondents wished to wash hands failed while 39.3% faced challenges related bathing. Failure was associated with received information on washing (APR = 1.14, 95% CI: 1.06-1.24), using piped water 0.88, 95%CI: 0.78-0.98) or a well as main source HCF 1.21,95% 1.03-1.42). Experiencing accessing services...

10.1080/09603123.2020.1755015 article EN International Journal of Environmental Health Research 2020-04-29

In this study, we sought to establish the prevalence of leptospirosis among renal patients and general outpatients attending Mulago National Referral Hospital, Uganda. A total 254 were recruited, their blood samples collected interviewer-administered semi-structured questionnaires provided between July October 2018. These captured data on sociodemographic characteristics symptoms disease. An individual with an average body temperature 37.3 ± 1.1 °C was considered be having fever. The...

10.1038/s41598-022-12544-3 article EN cc-by Scientific Reports 2022-05-19

Healthcare providers (HCPs) are at an elevated occupational health risk of hepatitis B virus infections. Post-exposure prophylaxis (PEP) is one the measures recommended to avert this risk. However, there limited evidence HCPs' awareness PEP. Therefore, study aimed establish PEP among HCPs in Wakiso, a peri-urban district that surrounds Uganda's capital, Kampala.A total 306 HCPs, selected from 55 healthcare facilities (HCFs) were interviewed using validated structured questionnaire. The data...

10.1371/journal.pone.0270181 article EN cc-by PLoS ONE 2022-06-23

Abstract Background The role of human, animal and environmental health workers is increasingly being recognized linked to the emergence transmission antimicrobial resistance (AMR). Little has been done in low middle-income countries despite seriousness this public challenge. Our study explored perspectives on occurrence, transmission, management AMR at human-animal-environment interface. Methods We conducted a descriptive qualitative within Wakiso district, central Uganda between August...

10.21203/rs.3.rs-2458964/v1 preprint EN cc-by Research Square (Research Square) 2023-01-12

South Sudan has endured decades of armed conflict, with the most recent in 2016. This left health system and infrastructure overstretched by a myriad infectious diseases like tuberculosis. Our study aimed at quantitatively qualitatively documenting TB dynamics challenges access to care during period civil unrest Wau. A cross sectional was carried out between January February 2016 Wau Teaching Hospital (WTH). Sputum randomly collected from 207 1035 suspects analyzed using Ziehl-Neelsen (ZN)...

10.1016/j.jctube.2018.06.001 article EN cc-by-nc-nd Journal of Clinical Tuberculosis and Other Mycobacterial Diseases 2018-07-18

Background: Ebola Virus Disease (EVD) presents with a high global mortality and is known to be highly infectious disease devastating gendered effects on the social fabric, yet most of science has focused disease’s biology. However, little been documented regard gender aspects in two sub counties (Kikyo Bundibugyo Town Council) District Western Uganda. The study was set examine differences level knowledge, attitudes perceptions about EVD. Methods: employed cross-sectional design using both...

10.4236/health.2019.111011 article EN Health 2019-01-01

Abstract Background Cryptosporidiosis is a common cause of diarrheal disease in livestock and dogs, it can result significant economic losses due to decreased productivity higher treatment costs. The cryptosporidiosis burden dogs largely unknown underexploited. We designed cross-sectional research study determine the prevalence factors associated with dogs. Methods questionnaire data was downloaded from Kobotoolbox server excel format for cleaning analysis. Laboratory results were matched...

10.1101/2023.02.27.23286549 preprint EN cc-by medRxiv (Cold Spring Harbor Laboratory) 2023-03-01

The occurrence of extended spectrum beta-lactamase (ESBL) producing bacteria such as Escherichia coli has increasingly become recognized beyond hospital settings. Resistance to other types antibiotics limits treatment options while the existence among humans, animals, and environment is suggestive potential zoonotic reverse-zoonotic transmission. This study aimed establish antibiotic susceptibility profiles ESBL-producing (ESBL-EC) from human, animal, environmental isolates obtained farming...

10.2147/idr.s398951 article EN cc-by-nc Infection and Drug Resistance 2023-04-01

Background: Brucellosis is the most common zoonotic infections in pastoral settings.Lack of knowledge about disease may cause devastating out come to patients, thus sustained it such communities.This study assessed and practices among high-risk groups Bahr el Ghazal region, South Sudan.Methods: Across sectional survey involving abattoir workers, febrile patients at Wau referral hospital cattle herders from camps four states namely, Wau, Tonj, Gogrial Aweil.By using open-ended questionnaire...

10.15761/crt.1000191 article EN Clinical Research and Trials 2017-01-01

<title>Abstract</title> Introduction: Exposure to <italic>Escherichia coli</italic> (<italic>E. coli</italic>) is a leading cause of diarrheal diseases, which pose significant problem in refugee settlements. Refugee populations are exposed feacal microorganisms through multiple pathways including sub-optimal sanitary facilities, contaminated drinking water, produce and food, flood bathing soil among others. While these well-documented, specific exposure behaviors remain underexplored. We...

10.21203/rs.3.rs-4414919/v1 preprint EN cc-by Research Square (Research Square) 2024-05-30

Abstract Introduction Exposure to Escherichia coli ( E. ) is a risk factor for diarrhoeal diseases, which pose significant problem in refugee settlements. Refugee populations are exposed faecal microorganisms through multiple pathways including sub-optimal sanitary facilities, contaminated drinking water, produce and food, flood bathing soil among others. While these well-documented, specific exposure behaviours remain underexplored. We assessed behaviour households Imvepi settlement,...

10.1186/s12889-024-19525-3 article EN cc-by BMC Public Health 2024-07-30

Abstract Background Extended-spectrum beta-lactamase-producing Escherichia coli (ESBL-PE) represents a significant global public health concern. Much as humans, animals and environments harbor ESBL-PE, its epidemiology in Uganda is still not well understood. This study explains the of ESBL-PE using one approach selected farming households Wakiso district, central Uganda. Methodology Environmental, human, animal samples were collected from 104 households. Additional data obtained observation...

10.1101/2022.11.12.22282228 preprint EN cc-by medRxiv (Cold Spring Harbor Laboratory) 2022-11-15

Background: Infectious diseases have proven to be among the greatest challenges life in Anthropocene era. Their repeated and cyclical occurrences coupled with varying variants, rapidly changing climatic conditions within this century threaten take world back “pre-development era” absence of corrective measures. We designed a research study determine influence weather parameters on spread COVID-19 Uganda from March 2020 January 2022.Methods: Records mortalities morbidities as well elements...

10.31223/x50373 preprint EN cc-by EarthArXiv (California Digital Library) 2023-07-14

Abstract Poor fruit handling practices causes physical damage to fruits and exposes them pathogenic microbial contamination with Salmonella spp , E. coli Vibrio . These contribute food borne illnesses such as Salmonellosis, Shigellosis, Cholera, O157:H7 infection Campylobacteriosis among others. An estimated 14 percent of all diseases registered at health centers are related in Uganda making this a public concern. The economic burden foodborne was about 300 million United States Dollars...

10.21203/rs.3.rs-1219774/v1 preprint EN cc-by Research Square (Research Square) 2022-01-03

Abstract Background Diarrheal diseases contribute greatly to the reported global childhood mortality and morbidity with related social, economic consequences. This study was conducted analyze utilization of Health Belief Model (HBM) theory comprehend diarrheal disease dynamics in Uganda. Methods Our utilized a qualitative cross-sectional design among adult livestock farmers selected farming communities. A total 80 individuals were recruited interviewed through Focus Discussion Groups (FDGs)...

10.1186/s12889-022-14413-0 article EN cc-by BMC Public Health 2022-11-02
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