A5039559033- Pancreatic function and diabetes
- Pancreatic and Hepatic Oncology Research
- Cannabis and Cannabinoid Research
- Pancreatitis Pathology and Treatment
- Organ Transplantation Techniques and Outcomes
- Diabetes Management and Research
- Diabetes and associated disorders
- Dietary Effects on Health
- Pneumocystis jirovecii pneumonia detection and treatment
- Immunodeficiency and Autoimmune Disorders
- HIV/AIDS Research and Interventions
- Bariatric Surgery and Outcomes
- Hernia repair and management
- Medical Imaging and Pathology Studies
- Ferroptosis and cancer prognosis
- Lung Cancer Diagnosis and Treatment
- Genomic variations and chromosomal abnormalities
- Assisted Reproductive Technology and Twin Pregnancy
- Prenatal Screening and Diagnostics
- Congenital Anomalies and Fetal Surgery
- Bipolar Disorder and Treatment
- Epigenetics and DNA Methylation
- Tracheal and airway disorders
- Diabetes Treatment and Management
- Adrenal and Paraganglionic Tumors
Universitair Ziekenhuis Brussel
2019-2024
Vrije Universiteit Brussel
2019-2023
Center for Beta Cell Therapy in Diabetes
2020
National Bank of Belgium
2020
Laboratoire de Génétique Cellulaire
2019
Abstract Maintenance of the pancreatic acinar cell phenotype suppresses tumor formation. Hence, repetitive acute or chronic pancreatitis, stress conditions in which cells dedifferentiate, predispose for cancer formation pancreas. Dedifferentiated acquire a large panel duct cell-specific markers. However, it remains unclear to what extent dedifferentiated acini differ from native and genes are uniquely regulating dedifferentiation. Moreover, most studies have been performed on mice since...
Europe is currently the most active region in field of pancreatic islet transplantation, and many leading groups are actually achieving similar good outcomes. Further collaborative advances require standardization cell product isolation processes, this work aimed to identify differences human processes within European countries.A web-based questionnaire about critical steps, including donor selection, pancreas processing, perfusion digestion, counting culture, quality evaluation,...
Objective The aggressive basal-like molecular subtype of pancreatic ductal adenocarcinoma (PDAC) harbours a ΔNp63 (p40) gene expression signature reminiscent basal cell type. Distinct from other epithelia with tumours, + cells reportedly do not exist in the normal pancreas. Design We evaluated human pancreas, chronic pancreatitis (CP) and PDAC. further studied depth non-cancerous tissue developed three-dimensional (3D) imaging protocol (FLIP-IT, Fluorescence Light sheet microscopic Imaging...
Acinar cell dedifferentiation is one of the most notable features acute and chronic pancreatitis. It can also be initial step that facilitates pancreatic cancer development. In present study, we further decipher precise mechanisms regulation using primary human cells murine experimental models. Our RNAseq analysis indicates that, in both species, early acinar accompanied by multiple pathways related to survival are highly enriched, where SLC7A11 (xCT) transiently upregulated. xCT specific...
Organs from donors after controlled circulatory death (DCD III) exhibit a higher risk for graft dysfunction due to an initial period of warm ischemia. This procurement condition can also affect the yield beta cells in islet isolates donor pancreases, and hence their use transplantation. The present study uses data collected generated by our Beta Cell Bank compare number DCD III (n = 141) with that brain (DBD, n 609), before culture, examines influence variables. cell per III-organ was...
Autoantibodies against islet cell antigens are routinely used to identify subjects at increased risk of symptomatic type 1 diabetes, but their relation the intra-islet pathogenetic process that leads positivity for these markers is poorly understood. We screened 556 non-diabetic organ donors (3 months 24 years) five different autoantibodies and found in 27 subjects, 25 single- two double autoantibody-positive donors. Histopathological screening pancreatic tissue samples showed lesion...
M2 macrophages play an important role in tissue repair and regeneration. They have also been found to modulate β-cell replication mouse models of pancreatic injury disease. We previously reported that is strongly increased a subgroup human organ donors characterized by prolonged duration stay intensive care unit (ICU) number leukocytes the tissue. In present study we investigated relationship between ICU, macrophages, vascularization, cell replication. Pancreatic organs from 50 without...
Patients fulfilling criteria for euthanasia can choose to donate their organs after circulatory death [donors (DCD V)]. This study assesses the outcome of islet cell isolation from DCD V pancreases. A procedure procurement provided 13 pancreases preserved in Institut Georges Lopez-1 preservation solution and following acirculatory warm ischemia time under 10 minutes. Islet outcomes are compared with those reference donors brain (DBD, n = 234) a cohort controlled III, 29) procured same...
Detection of amyloid in intraportal islet implants type 1 diabetes patients has been proposed as cause their functional decline. The present study uses cultured adult human islets devoid to examine conditions its formation. After injection patients, deposits <15 µm diameter were identified 5%-12% beta cell containing aggregates, 3-76 months posttransplant. Such also formed glucose-controlling the kidney diabetic mice but not failing implants. Alginate-encapsulated during culture when...
Abstract Acinar to ductal metaplasia (ADM) or acinar cell dedifferentiation is one of the most notable features chronic pancreatitis. It also considered initial step pancreatic cancer development when oncogenic mutations accumulate. However, its precise mechanism and regulatory pathways remain unclear. This study profiled transcriptome dedifferentiated cells from both humans mice. Differential expression analysis integrated with a systematic literature search revealed several potential...
ABSTRACT Maintenance of the pancreatic acinar cell phenotype suppresses tumor formation. Hence, repetitive acute or chronic pancreatitis, stress conditions in which cells dedifferentiate, predispose for cancer formation pancreas. Dedifferentiated acquire a large panel duct cell-specific markers. However, it remains unclear to what extent dedifferentiated acini differ from native and genes are uniquely regulating dedifferentiation. Moreover, most studies have been performed mice since...
Introduction: Both totally extraperitoneal herniorrhaphy (TEP) and spermatic vein embolization are minimal invasive techniques gaining in popularity the treatment of inguinal hernia varicocele respectively. A previous makes a TEP more difficult because fibrotic changes vein. This is to our knowledge first case report describing these difficulties after embolization. Case Presentation: We 37 year old male who presented with an uncomplicated left sided ipsilateral varicocele. He underwent...
Introduction: Islet transplant programs are highly dependent on quality donor pancreases that lead to beta cell isolates containing a sufficiently large number of functional cells. Donors after controlled circulatory death (DCD III) valuable source for expanding the organ pool but they considered at higher risk graft dysfunction due an initial period warm ischemia. The present study examines whether DCD III impairs pancreatic islet isolation and investigates procurement preservation limits...