A5072491174- Neuropeptides and Animal Physiology
- Trigeminal Neuralgia and Treatments
- Nerve injury and regeneration
- RNA Interference and Gene Delivery
- Pain Mechanisms and Treatments
- Bone and Dental Protein Studies
- Intracerebral and Subarachnoid Hemorrhage Research
- Head and Neck Surgical Oncology
- dental development and anomalies
- Oral microbiology and periodontitis research
- Advanced biosensing and bioanalysis techniques
- Dendrimers and Hyperbranched Polymers
- Apelin-related biomedical research
- Bone Metabolism and Diseases
- Protease and Inhibitor Mechanisms
- TGF-β signaling in diseases
- Mechanisms of cancer metastasis
- Neurosurgical Procedures and Complications
- Oral and gingival health research
- S100 Proteins and Annexins
- Pain Management and Placebo Effect
- Viral Infections and Immunology Research
- Kruppel-like factors research
- Children's Physical and Motor Development
- Ion channel regulation and function
Fujian Medical University
2012-2024
The University of Texas Health Science Center at San Antonio
2015-2024
Air Force Medical University
2011-2012
Abstract Dentin sialophosphoprotein (DSPP) is a dentin extracellular matrix protein that processed into sialoprotein (DSP), glycoprotein (DGP) and phosphoprotein (DPP). DSP mainly expressed in odontoblasts. We hypothesized interacts with cell surface receptors subsequently activates intracellular signaling. Using as bait for screening library, we demonstrate acts ligand binds to integrin β6. The 36 amino acid residues of are sufficient bind This peptide promoted attachment, migration,...
The central medial nucleus (CM), a prominent cell group of the intralaminar nuclei (ILN) thalamus, and ventrolateral periaqueductal gray matter (vlPAG) are two major components pain system. Whether vlPAG CM input sources nociceptive information to basolateral amygdala (BLA) whether they involved in neuropathic regulation remain unclear. Clarifying hierarchical organization these subcortical (vlPAG, CM, BLA) can enhance our understanding on neural circuits for regulation. Behavioral test...
Trigeminal neuralgia (TN) is a type of severe paroxysmal neuropathic pain commonly triggered by mild mechanical stimulation in the orofacial area. Piezo2, mechanically gated ion channel that mediates tactile allodynia pain, can be potentiated cyclic adenosine monophosphate (cAMP)-dependent signaling pathway involves exchange protein directly activated cAMP 1 (Epac1). To study whether Piezo2-mediated mechanotransduction contributes to peripheral sensitization rat model TN after trigeminal...
To investigate the function of cytokines, chemokines, and regulatory T cells (Tregs) in pathogenesis type 1 diabetes mellitus (T1DM) children.A total 35 children with T1DM 30 healthy controls were enrolled this study. Levels serum cytokines (IL-1α, IL-6, IL-10, IL-12, TNF-α) chemokines (MIP-1α, MIP-1α MCP-1) detected by enzyme-linked immunosorbent assay. Peripheral blood mononuclear (PBMCs) isolated culture supernatant phytohaemagglutinin (PHA)-stimulated PBMCs was subjected to ELISA for...
The trigeminal root entry zone (TREZ) is a transitional between the central nervous system (CNS) and peripheral (PNS), adjacent to brainstem. Microvascular compression of TREZ has been considered be primary etiology in most cases neuralgia (TN), but whether epigenetic regulation involved pathogenesis TN still unclear. Therefore, this study was designed investigate histone H3 acetylation an animal model TN.
Bmp2 is essential for dentin formation. cKO mice exhibited similar phenotype to dentinogenesis imperfecta, showing dental pulp exposure, hypomineralized dentin, and delayed odontoblast differentiation. As it relatively difficult obtain lot of primary papilla mesenchymal cells maintain a long‐term culture these cells, availability immortalized deleted critical studying the underlying mechanism signal in odontogenesis. In this study, our goal was generate an (iBmp2 ko/ko dp) cell line by...
Abstract Microvascular compression of the trigeminal root entry zone (TREZ) is main cause most primary neuralgia (TN), change glial plasticity was previously studied in TREZ TN rat model induced by chronic compression. To better understand role astrocytes and immune cells TREZ, different cell markers including fibrillary acidic protein (GFAP), complement C3, S100A10, CD45, CD11b, glutamate-aspartate transporter (GLAST), Iba-1 TMEM119 were used immunohistochemistry flow cytometry. On post...
Microvascular compression on the trigeminal root entry zone (TREZ) is main etiology of neuralgia (TN) patients. To investigate brain-derived neurotrophic factor (BDNF) and glial cell line-derived (GDNF) in ganglion (TG) TREZ, immunofluorescence staining Western blot were used a rat TN model. Both BDNF GDNF observed TG neurons TREZ. The expression dimer was increased group, while decreased after injury. dimer/pro-BDNF ratio TREZ group higher than that sham but significantly lower group. These...
Schwann cell and nerve conduit are crucial for regeneration re-myelination after peripheral nerves injury. To investigate the effects of Salidroside on autogenous epimysium mixed with BD matrigel RSC96 cells an animal model 5 mm sciatic defect injury in rat, motor function, muscle reinnervation, immunohistochemical staining, retrograded tracing Western blot were used this study. The results showed that enhanced compound to improve functional index gastrocnemius weight ratio, which better...
To investigate the expression of miR-218 and its clinical significance in osteosarcoma tissues explore effect on proliferation apoptosis cells.miR-218 was detected 76 samples surgically resected matched normal tumor-adjacent using quantitative reverse transcription polymerase chain reaction (qRT-PCR). MiR-218 over-expressed by exogenous plasmids Saos-2 cells, then BrdU cell assay flow cytometry were used to determine apoptosis.The significantly lower than those (t=8.735, P<0.001). tumor...
Bone morphogenetic proteins 2 and 4 (BMP2/4) are essential for osteoblast differentiation osteogenesis. Generation of a BMP2/4 dual knock-out ((ko/ko)) osteoblastic cell line is valuable asset studying effects on skeletal development. In this study, our goal was to create immortalized mouse deleted osteoblasts by infecting adenoviruses with Cre recombinase green fluorescent protein genes into murine floxed osteoblasts. Transduced BMP2/4(ko/ko) cells were verified immunofluorescence PCR....
To achieve biocompatibility and high gene transfection efficiency, developing new cationic polymers with natural functional moieties has been regarded as a promising way. In this work, histidine was grafted on chitosan (CS) to produce CS derivatives (HGCS) for siRNA delivery the HGCS/siRNA complexes were formed by self-assembly method. The structures of characterized FT-IR XRD. Acid-base titration assay used measure their proton buffering capacities results indicated HGCS have stronger...
The trigeminal root entry zone is the at which myelination switches from peripheral Schwann cells to central oligodendrocytes. Its special anatomical and physiological structure renders it susceptible nerve injury. etiology of most primary neuralgia closely related microvascular compression zone. This study aimed develop an efficient in vitro model mimicking glial environment as a tool investigate effects cell line-derived neurotrophic factor brain-derived on structural functional integrity...
Bmp2 is essential for dentin development and formation. conditional knock-out (KO) mice display a similar tooth phenotype of dentinogenesis imperfecta (DGI). To elucidate foundation subsequent functional studies cross talk between mRNAs lncRNAs in Bmp2-mediated dentinogenesis, we investigated the profiling using immortalized mouse dental flox/flox (iBmp2fx/fx) (iBmp2ko/ko) papilla cells. RNA sequencing was implemented to study expression mRNAs. Quantitative real-time PCR (RT-qPCR) used...
Abstract An ideal carrier that delivers small interfering RNA (siRNA) should be designed based on two criteria: high gene transfection efficiency and low cytotoxicity. In this work, arginine was used to modify polyvinylamine (PVAm) form a novel PVAm derivative (PVAm‐Arg) for siRNA delivery the characterized using Fourier transform infrared spectroscopy. PVAm‐Arg/siRNA complexes were formed by self‐assembly method. The zeta potential size of measured electrophoretic light scattering dynamic...
The aim of this study was to investigate the effect and possible mechanisms blood-nerve barrier (BNB) coagulation-anticoagulation system in modulating mechanical allodynia a trigeminal neuralgia (TN) rat model induced by chronic compression root entry zone (TREZ).Von Frey filaments were applied determine orofacial threshold. BNB permeability evaluated Evans blue extravasation test. Immunohistochemical staining laser confocal microscopy used measure length depletion zones nodes Ranvier TREZ,...
Jinshui He1, Xushuang Li2, Daoshu Luo3, Chaobao Zhang4, Shuanggang Hu4, and Xiangqi Li4* Department of Pediatrics, Zhangzhou Affiliated Hospital Fujian Medical University, 363000, China College Veterinary Medicine, Sichuan Agricultural Ya’an 625014, School Basic Sciences, Fuzhou 350004, Institute Biochemistry Cell Biology, Shanghai Institutes for Biological 200031, These authors contributed equally to this work. *Corresponding address. Tel: þ86-21-54921267; Fax: þ86-21-54921011; E-mail:...