- Biochemical effects in animals
- Free Radicals and Antioxidants
- Advanced Glycation End Products research
- Phytochemicals and Antioxidant Activities
- Redox biology and oxidative stress
- Electron Spin Resonance Studies
- Antioxidant Activity and Oxidative Stress
- Nitric Oxide and Endothelin Effects
- Vitamin C and Antioxidants Research
- Tea Polyphenols and Effects
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Sulfur Compounds in Biology
- Hemiptera Insect Studies
- Garlic and Onion Studies
- Collembola Taxonomy and Ecology Studies
- Drug Transport and Resistance Mechanisms
- Natural Antidiabetic Agents Studies
- Parkinson's Disease Mechanisms and Treatments
- Cancer, Hypoxia, and Metabolism
- Fermentation and Sensory Analysis
- Circadian rhythm and melatonin
- Genomics, phytochemicals, and oxidative stress
- Alcohol Consumption and Health Effects
- Metabolomics and Mass Spectrometry Studies
- Alzheimer's disease research and treatments
Rzeszów University
2016-2025
University of Silesia in Katowice
2003
Assays of total antioxidant capacity (TAC) are popular in the analysis food products. This review presents most assays TAC and their limitations, databases products, application clinical studies, effect processing on food. The importance sample preparation for striking effects digestion gastrointestinal tract discussed. Critical opinions validity considered. It is concluded that methods can be useful as screening quality control low-cost, high-throughput tools used to discover potential...
Nitroxides are stable free radicals that have antioxidant properties. They react with many types of radicals, including alkyl and peroxyl radicals. act as mimics superoxide dismutase stimulate the catalase activity hemoproteins. In some situations, they may exhibit pro-oxidant activity, mainly due to formation oxoammonium cations products their oxidation. this review, cellular effects nitroxides in animal experiments clinical trials discussed, beneficial various pathological situations...
The generation of hydrogen peroxide has been documented in various plant-based beverages, such as coffee, tea, herbal infusions and wine, well energy drinks containing ascorbate food. There are no data the literature on presence beer strong alcoholic beverages plant material. This study aimed to examine whether selected (brandy, whisky fruit liqueurs) contain peroxide. was found freshly opened brandy, whisky, liqueurs most diluted beers; subsequent incubation an air atmosphere led electron...
The aim of this study was to compare the kinetics glycoxidation bovine serum albumin (BSA) as a model protein by three sugars: glucose, fructose and ribose, using fluorometric measurements content advanced glycation end products (AGEs), protein-bound fructosamine, dityrosine, N'-formylkynurenine, kynurenine, tryptophan, oxidation (AOPP), carbonyl groups, well thiol groups. Moreover, levels glycoalbumin AGEs were determined an enzyme-linked immunosorbent assay. Based on kinetic results,...
Exposure to high glucose concentrations in vitro is often employed as a model for understanding erythrocyte modifications diabetes. However, effects of such experiments may be affected by consumption during prolonged incubation and changes cellular parameters conditioned impaired energy balance. The aim this study was compare alterations various red cell type experiment differentiate between those glycoxidation imbalance. Erythrocytes were incubated with 5, 45 or 100 mM up 72 h. High...
The aim of this study was to compare several methods for measurement bovine serum albumin (BSA) modification by glycoxidation with reactive dicarbonyl compounds (methylglyoxal ‒ MGO and glyoxal GO), studies the kinetics process effects 19 selected on BSA glycation aldehydes. results confirm higher reactivity respect GO point usefulness AGE, dityrosine N′-formylkynurenine fluorescence monitoring evaluation protection against glycation. Different extent induced found many compounds, probably...
The relationship between the prevalence of multiple sclerosis (MS) and sunlight's ultraviolet radiation was proved. Oxidative stress plays a role in pathogenic traits MS. Melatonin possesses antioxidative properties regulates circadian rhythms. Sleep disturbances MS patients are common contribute to daytime fatigue. aim study evaluate 5 mg daily melatonin supplementation over 90 days on serum total oxidant status (TOS), antioxidant capacity (TAC) its influence sleep quality depression level...
Dimethyl sulfoxide (DMSO) is used as a cryoprotectant for the preservation of cells, including yeast, and solvent chemical compounds. We report that DMSO induces oxidative stress in yeast. Saccharomyces cerevisiae wt strain EG-103 its mutants Δsod1, Δsod2, Δsod1 Δsod2 were used. Yeast subjected to action 1-14% 1 h at 28 °C. induced concentration-dependent inhibition yeast growth, effect being more pronounced devoid SOD (especially Δsod2). Cell viability was compromised....
A modification of the ABTS• decolorization assay for plate readers is presented. In our modification, 200 µL ABTS solution absorbance 1.0 at 734 nm was added with an antioxidant and decreased resulted. For comparison activities in kinetic decrease, concentration dependence decrease area under curve are recommended. “Fast” “slow” antioxidants were distinguished: while reactions “fast” completed within seconds, not finished after 6 min. We recommend reaction time 60 min assays such...