A5038126888- Advanced biosensing and bioanalysis techniques
- Biosensors and Analytical Detection
- Advanced Nanomaterials in Catalysis
- Electrochemical sensors and biosensors
- Gold and Silver Nanoparticles Synthesis and Applications
- Nanocluster Synthesis and Applications
- Electrochemical Analysis and Applications
- Advanced Biosensing Techniques and Applications
- RNA Interference and Gene Delivery
- Extracellular vesicles in disease
- Microfluidic and Capillary Electrophoresis Applications
- Analytical Chemistry and Sensors
- Plasmonic and Surface Plasmon Research
- Nanowire Synthesis and Applications
- Nanoporous metals and alloys
- DNA and Nucleic Acid Chemistry
- Innovative Microfluidic and Catalytic Techniques Innovation
- Conducting polymers and applications
- Copper-based nanomaterials and applications
- Carbon and Quantum Dots Applications
- MicroRNA in disease regulation
- 3D Printing in Biomedical Research
- SARS-CoV-2 and COVID-19 Research
Auburn University
2021-2025
Shenzhen University
2022-2024
University of Central Florida
2019-2021
Fuzhou University
2011-2017
Michigan Technological University
2017
Houghton University
2017
Au nanoparticles (AuNPs) as signal reporters have been utilized in colorimetric vitro diagnostics (IVDs) for decades. Nevertheless, it remains a grand challenge to substantially enhance the detection sensitivity of AuNP-based IVDs confined by inherent plasmonics AuNPs. In this work, we circumvent confinement developing unique dual-functional AuNPs that were engineered coating conventional with ultrathin Pt skins sub-10 atomic layers (i.e., Au@Pt NPs). The NPs retain plasmonic activity...
Nanostructure-based visual assay has been developed for determination of enzymatic activity, but most involve in poor visible color resolution and are not suitable routine utilization. Herein, we designed a high-resolution colorimetric protocol based on gold/silver core/shell nanorod readout alkaline phosphatase (ALP) activity by using bare-eyes. The method relied reaction-assisted silver deposition gold to generate significant change, which was strongly dependent ALP activity. Upon target...
A novel reverse colorimetric immunoassay (RCIA) strategy was for the first time designed and utilized sensitive detection of low-abundance protein (prostate-specific antigen, PSA, used in this case) biological fluids by coupling highly catalytic efficient catalase with magnetic bead-based peroxidase mimics. To construct such a RCIA system, two nanostructures including beads gold nanoparticles were synthesized functionalized anti-PSA capture antibody catalase/anti-PSA antibody, respectively....
Herein, gold–silver bimetallic nanoclusters (Au–Ag NCs) with the high fluorescent intensity were first synthesized successfully and utilized for fabrication of sensitive specific sensing probes toward inorganic pyrophosphatase (PPase) activity help copper ion (Cu2+) pyrophosphate (PPi). Cu2+ was used as quencher Au–Ag NC, while PPi employed hydrolytic substrate PPase. The system consisted PPi, ion, bovine serum albumin (BSA)-stabilized NC. detection carried out by enzyme-induced hydrolysis...
Methods based on enzyme labels have been developed for colorimetric immunoassays, but most involve poor sensitivity and are unsuitable routine use. Herein, we design an enhanced immunoassay prostate-specific antigen (PSA) coupling with enzyme-cascade-amplification strategy (ECAS-CIA). In the presence of target PSA, labeled alkaline phosphatase secondary antibody catalyzes formation palladium nanostructures, which catalyze 3,3′,5,5′-tetramethylbenzidine-H2O2 system to produce colored...
A new impedimetric immunoassay protocol based on enzyme-triggered formation of tyramine-enzyme repeats gold nanoparticle (AuNP) was designed for highly sensitive detection carcinoembryonic antigen (CEA, as a model) by virtue utilizing enzymatic biocatalytic precipitation toward 4-chloro-1-naphthol (4-CN) anti-CEA antibody (Ab1)-modified immunosensor. Initially, AuNP functionalized with horseradish peroxidase and (HRP-AuNP-Ab2), then HRP–tyramine conjugate utilized the tyramine–HRP through...
Although various colorimetric methods have been actively developed for the detection of Ag+ ions because their simplicity and reliability, limits these are confined to nanomolar (nM) level. Here, we demonstrate a novel strategy with picomolar (pM) sensitivity. This involves use poly(vinylpyrrolidone)- (PVP-) capped Pt nanocubes as artificial peroxidases that can effectively generate colored signal by catalyzing oxidation peroxidase substrates. In presence ions, generated cubes is greatly...
While various effects of physicochemical parameters (e.g., size, facet, composition, and internal structure) on the catalytic efficiency nanozymes (i.e., nanoscale enzyme mimics) have been studied, strain effect has never reported understood before. Herein, we demonstrate in by using Pd octahedra icosahedra with peroxidase-like activities as a model system. Strained were found to display 2-fold higher than unstrained octahedra. Theoretical analysis suggests that tensile is more beneficial OH...
Colorimetric assay platforms for dissolved hydrogen sulfide (H2S) have been developed more than 100 years, but most still suffer from relatively low sensitivity. One promising route out of this predicament relies on the design efficient signal amplification methods. Herein, we rationally designed an unprecedented H2S-induced deactivation (gold core)@(ultrathin platinum shell) nanocatalysts (Au@TPt-NCs) as a highly method ultrasensitive headspace-colorimetric H2S. Upon target introduction,...
Current methods for tuning the plasmonic properties of metallic nanoparticles typically rely on alternating morphology (i.e., size and/or shape) nanoparticles. The variation oftentimes impairs their performance in certain applications. In this study, we report an effective approach based control internal structure to engineer morphology-invariant with tunable properties. Specifically, these were prepared through selective growth Ag inner surfaces preformed Ag–Au alloyed nanocages as seeds...
Tumor-derived exosomes play a vital role in the process of cancer development. Quantitative analysis and exosome-shuttled proteins would be immense value understanding progression generating reliable predictive biomarkers for diagnosis treatment. Recent studies have indicated critical exosomal programmed death ligand 1 (PD-L1) immune checkpoint therapy its application as patient stratification biomarker immunotherapy. Here, we present nanoplasmonic exosome immunoassay utilizing gold-silver...
Cytokine storm, known as an exaggerated hyperactive immune response characterized by elevated release of cytokines, has been described a feature associated with life-threatening complications in COVID-19 patients. A critical evaluation cytokine storm and its mechanistic linkage to requires innovative immunoassay technology capable rapid, sensitive, selective detection multiple cytokines across wide dynamic range at high-throughput. In this study, we report machine-learning-assisted...
A novel colorimetric immunoassay is designed for the ultrasensitive detection of human prostate-specific antigen (PSA) by using a unique type nanolabel – high-index {<italic>hk</italic>0} faceted platinum concave nanocubes.
The ability to produce a diverse spectrum of hollow nanostructures is central the advances in many current and emerging areas technology. Herein, we report general method craft with highly tunable physical chemical parameters. key strategy regenerate nanoscale sacrificial templates galvanic replacement reaction through site-selective overgrowth. As examples, demonstrate syntheses nanocages nanotubes made silver, gold, palladium, and/or platinum well-controlled wall thicknesses elemental...
A facile and simple electrochemical immunoassay for ultrasensitive determination of streptomycin residues (STR) in food was designed by using nanogold-assembled mesoporous silica (GMSNs) as bionanolabels on a three-dimensional redox-active organosilica-functionalized sensing interface. To construct such interface, we initially synthesized organosilica colloids wet chemical method, then utilized the prepared colloidal nanocomposites immobilization monoclonal anti-STR antibodies glassy carbon...