Abstract Angular scanning-based surface plasmon resonance measurement has been utilized in label-free sensing applications. However, the accuracy and precision of measurements rely on an accurate plasmonic angle. Several methods have proposed reported literature to measure angle, including polynomial curve fitting, image processing, averaging. For intensity detection, limit SPR is around 10 –5 RIU –6 RIU. Here, we propose a deep learning-based method locate angle enhance detection without...

Surface Plasmon microscopy can measure local changes of refractive index on the micron scale.Interferometric plasmon imaging delivers quantitative high spatial resolution sensitive to index.In addition so called V(z) method allows image contrast be controlled by varying sample defocus without substantially degrading resolution.Here, we show how a confocal system provides simpler and more stable alternative.This system, however, places greater demands dynamic range system.We therefore use...

We describe the construction of a prismless widefield surface plasmon microscope; this has been applied to imaging interactions protein and antibodies in aqueous media. The illumination angle spatially incoherent diffuse laser was controlled with an amplitude spatial light modulator placed conjugate back focal plane allow dynamic control angle. Quantitative microscopy images high resolution were acquired by post-processing series obtained as function Experimental results are presented...

Abstract A deep learning algorithm for single-shot phase retrieval under a conventional microscope is proposed and investigated. The has been developed using the context aggregation network architecture; it requires single input grayscale image to predict an output profile through learning-based pattern recognition. Surface plasmon resonance imaging employed as example demonstrate capability of method. profiles surface phenomena have very well established cover ranges transitions from 0 2π...

As disease diagnosis becomes more sophisticated, there is a requirement to measure small numbers of molecules attached to, for instance, an antibody. This requires sensor capable not only high sensitivity but also the ability make measurements over highly localized region. In previous publications, we have shown how modified confocal microscope allows one surface plasmon (SP) on scale far smaller than propagation distance. The present implementation presents new ultrastable interferometer...

In previous publications [Opt. Express 20, 7388 (2012), Opt. 28039 (2012)] we showed how a confocal configuration can form an surface plasmon microscope involving interference between path the generation of plasmons and one directly reflected beam. The relative phase these contributions changes with axial scan position allowing velocity to be measured. this paper extend interferometer concept produce 'embedded' shifting interferometer, where control reference beams spatial light modulator....

Summary This paper develops the theoretical framework to understand capability of interferometric surface plasmon microscope quantify sample properties in a confined region. We use rigorous diffraction theory ability system measure local and eliminate crosstalk from adjacent regions. argue that defocused condition defines measured point excitation reradiation plasmons; which greatly improves localisation. also present results for noninterferometric microscope, confirm based can perform...

This paper applies rigorous diffraction theory to evaluate the minimum mass sensitivity of a confocal optical microscope designed excite and detect surface plasmons operating on planar metallic substrate.The model is compared with an intuitive ray picture which gives remarkably similar predictions.The combination focusing accurate phase measurement mean that under favorable but achievable conditions detection small numbers molecules possible, however, we argue reliable single will benefit...

We investigate the performance of surface plasmon and Fabry-Perot modes formed between two closely spaced layers. The motivation for this study is twofold: first, to look that may be excited at lower incident angles compared usual Kretschmann configuration with similar or superior refractive index responsivity and, second, develop a simple applicable method these structures over wide range separations without recourse construction ad hoc structures. Using back focal plane observation...

We demonstrate numerically through rigorous coupled wave analysis (RCWA) that replacing the prism in Otto configuration with gratings enables us to excite and control different modes field patterns of surface phonon polaritons (SPhPs) incident wavelength height spacing layer.This modified provides following multiple modes, namely, SPhP mode, Fabry-Pérot (FP) cavity resonance, dielectric waveguide grating resonance (DWGR) hybridized between combinations above mentioned modes.We show this...

In this paper, we present a direct method to measure surface wave attenuation arising from both ohmic and coupling losses using our recently developed phase spatial light modulator (phase-SLM) based confocal plasmon microscope. The measurement is carried out in the far-field phase-SLM impose an artificial profile back focal plane (BFP) of microscope objective. other words, effectively provide artificially engineered backward by modulating Goos Hänchen (GH) shift wave. Such waves with...

In this paper, we report a theoretical framework on the effect of multiple resonances inside dielectric cavity insulator-insulator-metal-insulator (IIMI)-based surface plasmon sensors. It has been very well established that structure can support both long-range polaritons (LRSPP) and short-range (SRSPP). We found resonant under certain conditions be employed as resonator to enhance LRSPP properties. These are: (1) refractive index was greater than sample layer (2) when light propagated in...

We report a theoretical framework to explain the characteristics of Fabry-Pérot (FP) resonances excited in thin film-based grating consisting gold layer and rectangular dielectric sub-wavelength near-wavelength regimes. The zeroth-order diffraction inside forms an FP resonant cavity with effective refractive index arising from averaging effect between indices material filling grooves. A simplified model based on Fresnel equations phase matching condition is proposed predict mode for...

This paper provides a theoretical framework to analyze and quantify roughness effects on sensing performance parameters of surface plasmon resonance measurements. Rigorous coupled-wave analysis the Monte Carlo method were applied compute plasmonic reflectance spectra for different profiles. The rough surfaces generated using low pass frequency filtering method. Different coating treatments their reported root-mean-square in literature extracted investigated this study calculate refractive...

In this paper, a database of biomass production from Yarrowia lipolytica fermentation is prepared and constructed using machine learning data mining approaches. The curated 15 publications consists 301 rows with 25 predictors 1 label. include inoculum size, temperature, pH, time, while the label corresponding production. then divided into training, validation, test datasets analyzed as supervised task for regression. Twenty-six regression models are employed compared their performance in...

Surface Plasmon microscopy enables measurement of local refractive index on a far finer scale than prism based systems.An interferometric or confocal system gives the so-called V(z) curve when sample is scanned axially, which measure surface plasmon propagation velocity.We show how phase spatial light modulator (i) performs necessary pupil function apodization (ii) imposes an angular varying shift that effectively changes defocus without any mechanical movement and (iii) relative reference...

This research proposes an algorithm to preprocess photoplethysmography (PPG) and electrocardiogram (ECG) signals apply the processed context aggregation network-based deep learning achieve higher accuracy of continuous systolic diastolic blood pressure monitoring than other reported algorithms. The preprocessing method consists following steps: (1) acquiring PPG ECG for a two second window at sampling rate 125 Hz; (2) separating into array 250 data points corresponding 2 s window; (3)...

In previous work we demonstrated how a confocal microscope with spatial light modulator in the back focal plane could perform accurate measurement of k-vector propagating surface plasmon. This involved forming an embedded interferometer between incident close to normal incidence (reference beam) and at angle excite plasmons (sample beam). The signal from was extracted by stepping phase reference beam relative sample using modulator; this requires least 3 steps, which limits speed operation....

Surface plasmon resonance (SPR) has been utilized in various optical applications, including biosensors. The SPR-based sensor is a gold standard for protein kinetic measurement due to its ultrasensitivity on the plasmonic metal surface. However, slight change surface morphology, such as roughness or pattern, can significantly impact performance. This study proposes theoretical framework explain sensing mechanisms and quantify performance parameters of angular detection binding at different...

Filtration of biological liquids has been widely employed in biological, medical, and environmental investigations due to its convenience; many could be performed without energy on-site, particularly protein separation. However, most available membranes are universal absorption or sub-fractionation molecule sizes properties. SPMA, syringe-push membrane absorption, is a quick easy way prepare biofluids for evaluation. The idea initiating SPMA was filter proteins from human urine subsequent...

Surface plasmon Resonance (SPR) has recently been of interest for label-free voltage sensing. Several SPR structures have proposed. However, making a quantitative cross-platform comparison these is not straightforward due to (1) different measurement mechanisms; (2) electrolytic solution and concentration in the measurement; (3) levels external applied potential. Here, we propose approach make direct across structures, solutions mechanisms. There are two employed as example this theoretical...

We propose a theoretical framework to analyze quantitative sensing performance parameters, including sensitivity, full width at half maximum, plasmonic dip position, and figure of merits for different surface plasmon operating conditions Kretschmann configuration. Several definitions expressions the merit have been reported in literature. Moreover, optimal each are, fact, different. In addition, there is still no direct comparison between identify which definition provides more accurate...