A5050184215- Cell Image Analysis Techniques
- Single-cell and spatial transcriptomics
- Advanced Vision and Imaging
- Advanced Fluorescence Microscopy Techniques
- Amyotrophic Lateral Sclerosis Research
- Neurogenetic and Muscular Disorders Research
- Video Surveillance and Tracking Methods
- Spectroscopy Techniques in Biomedical and Chemical Research
- Muscle Physiology and Disorders
- Bacillus and Francisella bacterial research
- Graphene research and applications
- Advanced biosensing and bioanalysis techniques
- Image Processing Techniques and Applications
- Biomedical and Engineering Education
- Immunotherapy and Immune Responses
- Graphene and Nanomaterials Applications
Gladstone Institutes
2017-2023
University of California, San Francisco
2017-2023
Theragnostic Technologies (United States)
2017
Stony Brook Medicine
2017
We have developed a novel oxidized graphene nanoribbon-based platform (O-GNR) for gene delivery of double-stranded DNA into mammalian cells. O-GNRs, synthesized via longitudinal unzipping multi-walled carbon nanotubes (MWCNTs), exhibited efficient loading small dsDNA fragments. Fourier Transform Infrared Spectroscopy identified stretching peaks in the O-P-O and sugar phosphate backbone that were consistent with onto O-GNRs. The presence salts buffer promoted effective dispersion DNA:O-GNR...
The National Institute of Health (NIH) Library integrated network-based cellular signatures (LINCS) program is premised on the generation a publicly available data resource cell-based biochemical responses or "signatures" to genetic environmental perturbations. NeuroLINCS uses human inducible pluripotent stem cells (hiPSCs), derived from patients and healthy controls, differentiated into motor neuron cell cultures. This multi-laboratory effort strives establish i) robust multi-omic workflows...
High-throughput microscopy has outpaced analysis; biomarker-optimized CNNs are a generalizable, fast, and interpretable solution.
Live-cell imaging is an important technique to study cell migration and proliferation as well image-based profiling of drug perturbations over time. To gain biological insights from live-cell data, it necessary identify individual cells, follow them time extract quantitative information. However, since often experiment does not allow the high temporal resolution reduce excessive levels illumination or minimize unnecessary oversampling monitor long-term dynamics, still a challenging task...
Abstract Cell death is an essential process in biology that must be accounted for live microscopy experiments. Nevertheless, cell difficult to detect without perturbing experiments with stains, dyes or biosensors can bias experimental outcomes, lead inconsistent results, and reduce the number of processes simultaneously labelled. These additional steps also make scale high-throughput screening because cost, labor, analysis they entail. We address this fundamental limitation...
Abstract Live-cell imaging is an important technique to study cell migration and proliferation as well image-based profiling of drug perturbations over time. To gain biological insights from live-cell data, it necessary identify individual cells, follow them time extract quantitative information. However, since often experiment does not allow the high temporal resolution reduce excessive levels illumination or minimize unnecessary oversampling monitor long-term dynamics, still a challenging...