Microalgal lipids are a source of valuable nutritional ingredients in biotechnological industries, and precursors to biodiesel production. Here, the effects salt-induced stresses, including NaCl, KCl, LiCl on production lipid green microalga Chlamydomonas reinhardtii (137c) were investigated. NaCl stress dramatically increased saturated fatty acids (SFAs), which accounted for 70.2% acid methyl ester (FAMEs) under stress. In contrary, KCl led slight increase SFAs (47.05%) with remaining being...

This paper describes, in detail, the development of a novel, low-cost, and flexible drift tube (DT) along with an associated ion mobility spectrometer system. The DT is constructed from printed circuit board (PCB), bespoke "dog-leg" track design, that can be rolled up for ease assembly. approach incorporates shielding layer, as part PCB represents minimum dimensional footprint conceivable DT. low thermal mass polyimide substrate overlapping electrodes, afforded by dog-leg allow efficient...

A method for the synthesis of small molecule macroarrays N-acylated l-homoserine lactones (AHLs) is reported. focused library AHLs was constructed, and macroarray platform found to be compatible with both solution agar-overlay assays using quorum-sensing (QS) reporter strains. Several QS antagonists were discovered serve showcase as a straightforward technique research.

Abstract Microalgae are a diverse group of photosynthetic eukaryotic organisms that widely distributed globally. They prolific sources highly valuable compounds with fascinating chemical structures. Due to their balanced nutritional compositions and health benefits, they increasingly being used as functional food ingredients. Carotenoid‐based pigments polyunsaturated fatty acids ( PUFA s) examples high‐value nutrients can be accumulated abundantly in microalgae. Here, the effects potassium...

Bacteria frequently manifest distinct phenotypes as a function of cell density in phenomenon known quorum sensing (QS). This intercellular signalling process is mediated by "chemical languages" comprised low-molecular weight signals, autoinducers, and their cognate receptor proteins. As many the regulated QS can have significant impact on success pathogenic or mutualistic prokaryotic–eukaryotic interactions, there considerable interest methods to probe modulate pathways with temporal spatial...

With the rising trend of valuing flavor complexity coffees, means to distinguish properties individual coffee sources is vital sustainable growth industry. Herein, paper spray mass spectrometry (PS-MS), a simple technique with little sample preparation, was used collect data from aqueous extracts coffees various sources. Thereafter, principal component analysis and linear discriminant were successfully classify samples (with 80-100 % accuracy) studies including differentiations Arabica...

Tetrakis(4-sulfonatophenyl)porphyrin (TSPP) was immobilized on patterned paper and used as a sensor for heavy metal ions in an advanced organic chemistry course. The resulting could detect Hg2+ Cd2+ colorimetrically, while Cu2+ ion resulted fluorescence quenching, thus demonstrating multiplex capability of this paper-based sensor. From the synthesis porphyrin to fabrication sensor, undergraduate students gained first-hand experience with classical synthesis, also witnessed its application...

We report herein a practical method for nonlethal detection of the antibiotic sulfamethazine in pig body fluids via combination simple extraction and paper spray mass spectrometry (PS-MS). This requires minimal sample preparation while still providing high sensitivities accuracies complex matrices including whole blood (LOD = 7.9 μg/L; recovery 95.4–103.7%), serum 11.5 103.2–106.2%), synthetic urine 11.2 99.1–103.2%). Given known correlation between level edible tissues, this shows great...

“Immobilisation of pyrrolidinyl peptide nucleic acids on paper resulted in a new DNA sensor with great specificity”.

The utilization of microalgae as a source carotenoid productions has gained increasing popularity due to its advantages, such relatively fast turnaround time. In this study, newly discovered Coelastrum sp. TISTR 9501RE was characterized and investigated for taxonomical identity profile. To the best our knowledge, report first fully investigate profiles in microalga genus Coelastrum. Upon use limited nutrients stress condition, strain able produce astaxanthin, canthaxanthin, lutein, major...

Abstract A cationic porphyrin, 5,10,15,20‐tetrakis(1‐methyl‐4‐pyridinio)porphyrin tetraiodide ( TMPyP ) was reported herein to exert selective, turn‐off fluorescence when complexed with Cu 2+ ion. Although discernible spectroscopic changes of charged porphyrins various metal ions were previously utilized as colorimetric sensors, the application quenching ion has never been investigated in much detail before. Coupled ease synthesis, finding led a construction simple paper‐based sensor for...

Numerous novel methods to detect foodborne pathogens have been extensively developed ensure food safety. Among the important bacteria, Bacillus cereus was identified as a pathogen of concern that causes various illnesses, leading interest in developing effective detection for this pathogen. Although standard method based on culturing and biochemical confirmative test is available, it time- labor-intensive. Alternative PCR-based but lack high-throughput capacity ease use. This study,...

A new set of lipidated peptides was <italic>in situ</italic> synthesised on cotton surface, resulting in non-leaching surfaces with high antibacterial activities Gram-negative bacteria. These were also shown to be non-toxic mammalian cells.

Paper spray ionization (PSI) mass spectrometry (MS) is an emerging tool for ambient reaction monitoring via microdroplet acceleration. PSI-MS was used to accelerate and monitor the time course of dansyl chloride with aniline, in acetonitrile, produce aniline. Three distinct PSI arrangements were explored this study representing alternative approaches sample loading interaction; conventional single tip as well two novel setups, a dual-tip co-axial arrangement designed so limit any on-paper...

With increasing demands for more rapid and practical analyses, various techniques of ambient ionization mass spectrometry have gained significant interest due to the speed analysis abundance information provided. Herein, an technique that utilizes corona discharge was applied, first time, analyze categorize whole seeds black white peppers from different origins. This setup requires no solvent application nor gas flow, thus resulting in a very simple can be applied directly sample without any...

Abstract The popularity and high price of durian make quality control in terms ripeness very important, which turn depends heavily on harvesting at an appropriate maturity stage. To date, reports data-driven methods for prediction are scarce, with many rather focusing prediction. Herein, we report the first disclosure key molecular markers liquid extract peduncle that can be a predictive tool maturity. Multiple chromatographic spectroscopic techniques including TLC, HPLC, PS-MS, LC–MS/MS,...

In the past few decades, chemistry has evolved to interact with various disciplines synergistically help tackle global challenges. This, in turn, requires that newer generations of students are trained be more flexible accepting and coordinating new concepts. this experiment, pineapple was used as a key model allow for incorporation multiple important concepts into laboratory. Using carotenoid separation main goal, column chromatography, UV–vis spectroscopy, thin-layer high-performance...

A macroarray immobilisation of fluorophores on filter papers for sensing metal ions by in-situ reductive amination and carbodiimide coupling is reported herein. Chemometric approaches resulted in a rapid discovery sensors that can synergistically discriminate up to 12 with great prediction accuracies. Covalently bound paper, sensoring scaffolds were synthesised from the format readily be adopted as practical paper-based reusability sensitivity, achieving limit detection at low nanomolar...

Abstract A new displacement probe based on pyrrolidinyl peptide nucleic acid was designed and evaluated for DNA sequence recognition. The prepared by combining an N ‐terminally fluorophore‐modified (Flu‐ or TMR‐acpcPNA) a 3’‐Dabcyl‐modified as quencher. Fluorescence studies showed that the fluorophore in acpcPNA strand efficiently quenched quencher strand. After some optimisation, fluorescence significantly restored upon addition of complementary target, while stayed at low level with...