A5011755961- Bacteriophages and microbial interactions
- Monoclonal and Polyclonal Antibodies Research
- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- COVID-19 epidemiological studies
- Glycosylation and Glycoproteins Research
- Viral Infections and Vectors
- Transgenic Plants and Applications
- Vector-borne infectious diseases
- Mosquito-borne diseases and control
- Probiotics and Fermented Foods
- Genomics and Phylogenetic Studies
- Infant Nutrition and Health
- Clostridium difficile and Clostridium perfringens research
- Respiratory viral infections research
- Protein purification and stability
- Escherichia coli research studies
- RNA modifications and cancer
- Gut microbiota and health
- Microbial Community Ecology and Physiology
- SARS-CoV-2 detection and testing
- COVID-19 Impact on Reproduction
Institute of Immunology
2024
Łukasiewicz Research Network – PORT Polish Center for Technology Development
2021-2023
Ludwik Hirszfeld Institute of Immunology and Experimental Therapy
2020-2023
Polish Academy of Sciences
2020-2023
Wroclaw Research Centre EIT+ (Poland)
2021-2023
Introduction: Increasing number of deaths from multi-drug resistant bacterial infections has caused both the World Health Organization and Centers for Disease Control Prevention to repeatedly call development new, non-traditional antibacterial treatments. Antimicrobial enzymes, including those derived bacteriophages, known as endolysins or enzybiotics, are considered promising solutions among emerging therapies. These naturally occurring proteins specifically destroy cell walls...
Endolysins are bacteriolytic enzymes derived from bacteriophages. They represent an alternative to antibiotics, since they not susceptible conventional antimicrobial resistance mechanisms. Since non-human proteins efficient inducers of specific immune responses, including the IgG response or development allergic mediated by IgE, we evaluated general immunogenicity highly active antibacterial enzyme, PlyC, in a human population and mouse model. The study includes identification molecular...
Background: Bacteriophages may induce specific antibodies after natural exposure to phages or phage therapy. As such, phage-specific might impact bioavailability in vivo, although limited non-neutralizing insignificant effects have also been reported. Materials and Methods: Here, we report antibody induction against PB1-related (Pseudomonas viruses LMA2, F8, DP1) mice over an 80-day period, for a healthy population of humans, patients undergoing therapy (oral and/or topical treatment)....
Abstract Predictors for the risk of severe COVID-19 are crucial patient care and control disease. Other infectious diseases as potential comorbidities in SARS-CoV-2 infection still poorly understood. Here we identify association between course Lyme disease (borreliosis), caused by Borrelia burgdorferi transmitted to humans ticks. Exposure was identified multi-antigenic (19 antigens) serological testing patients: (hospitalized), asymptomatic mild (home treated or not aware being infected),...
Population immunity (herd immunity) to SARS-CoV-2 derives from two sources: vaccinations or cases of infection with the virus. Infections can be diagnosed as COVID-19 and registered, they asymptomatic, oligosymptomatic, even full-blown but undiagnosed unregistered when patients recovered at home. Estimation population is difficult remains a subject speculations. Here we present screening for specific IgG IgA antibodies in Polish citizens (N = 501) who had never been positively vaccinated...
Bacteriolytic enzymes are promising antibacterial agents, but they can cause a typical immune response in vivo. In this study, we used targeted modification method for two endolysins, Pal and Cpl-1. We identified the key immunogenic amino acids, designed tested new, bacteriolytic variants with altered immunogenicity. One new variant of (257-259 MKS → TFG) demonstrated decreased immunogenicity while similar mutant TFK) increased A third (280-282 DKP GGA) significantly activity it was not...
The immune response and specific antibody production in COVID-19 are among the key factors that determine both prognostics for individual patients global perspective controlling pandemics. So called "dark figure", is, a part of population has been infected but not registered by health care system, make it difficult to estimate herd immunity predict pandemic trajectories. Here we present follow up study screening hidden SARS-CoV-2 individuals who had never positively diagnosed against...
Abstract Certain parts of the human body, like gut, serve as ecological niches where bacteriophages thrive. In contrast, blood is not typically expected to host phageomes. However, have been shown translocate into bloodstream. The factors determining why some phages within phageomes efficiently while others do remain unclear. We investigated translocation phageome from gut mucosa bloodstream using bioptates large intestine collected via endoscopy (VLPs fraction) and serum samples (total...
Abstract The microbiota plays a key role for human health, and microbiome composition has been linked to specific health disorders many times. Different fractions of the enter dynamic interactions, particularly bacteria bacteriophages (phages) – viruses preying on bacteria. Since exists inside body, body strongly affects microbes, although not in uniform way, but shaped by extreme diversity genetic variants. These triadic dynamics bacterial microbiome, phageome, host genotype remain poorly...
Multiple pathogens are competing against the human immune response, leading to outbreaks that increasingly difficult control. For example, SARS-CoV-2 virus continually evolves, giving rise new variants. The ability evade system is a crucial factor contributing spread of these variants within population. With continuous emergence variants, it challenging comprehend all possible combinations previous infections, various vaccination types, and potential exposure in an individual patient. Rather...
Abstract Predictors for the risk of severe COVID-19 are crucial patient care and control disease. Other infectious diseases as potential comorbidities in SARS-CoV-2 infection still poorly understood. Here we identify association between course Lyme disease (borreliosis), caused by Borrelia burgdorferi transmitted to humans ticks. Exposure was identified multi-antigenic (19 antigens) serological testing patients: (hospitalized), asymptomatic mild (home treated or not aware being infected),...
Abstract The immune response and specific antibody production in COVID-19 are among the key factors that determine both prognostics for individual patients global perspective controlling pandemics. So called “dark figure”, is, a part of population has been infected but not registered by health care system, make it difficult to estimate herd immunity predict pandemic trajectories. Here we present follow up study screening hidden SARS-CoV-2 individuals who had never positively diagnosed...
Abstract Bacteriolytic enzymes are promising antibacterial agents, but they can cause a typical immune response in vivo . In this study, we used targeted modification of two endolysins, Pal and Cpl-1. We identified the key immunogenic amino-acids, designed tested new, bacteriolytic variants with altered immunogenicity. One new variant (257-259 MKS → TFG) demonstrated decreased immunogenicity while similar mutant TFK) increased A third (280-282 DKP GGA) significantly activity it was not...
Abstract The major factor that shapes the global perspective for increase or diminution of successive pandemic waves COVID-19 is immunological protection. SARS-CoV-2 virus constantly develops new variants, and capability immune evasion among factors promote variant spreading in human population. After two years evolution, it almost impossible to explain effects all possible combinations different viral strains, a few types vaccinations variants infecting an individual patient. Instead...
Abstract Population immunity (herd immunity) to SARS-CoV-2 derives from two well-defined and controlled sources: vaccinations or diagnosed registered cases of the disease. It may however also result asymptomatic, oligosymptomatic, even full-blown but undiagnosed unregistered which patients recovered at home. Here we present a population screening for specific IgG IgA antibodies in Polish citizens (N=501) who had never been positively with vaccinated against SARS-CoV-2. Serum samples were...