A5031167498- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- RNA Interference and Gene Delivery
- CRISPR and Genetic Engineering
- RNA Research and Splicing
- Technology and Security Systems
- Bacterial Genetics and Biotechnology
University of Massachusetts Amherst
2015-2023
Abstract T7 RNA polymerase is commonly used to synthesize large quantities of for a wide variety applications, from basic science mRNA therapeutics. This in vitro system, while showing high fidelity many ways, also well known producing longer than encoded products, particularly under high-yield reaction conditions. Specifically, the resulting product pool contaminated by an often disperse collection cis-primed extension products. In addition reducing yield via conversion correctly...
High yields of RNA are routinely prepared following the two-step approach high-yield in vitro transcription using T7 polymerase followed by extensive purification gel separation or chromatographic methods. We recently demonstrated that reactions, as accumulates solution, rebinds and extends encoded (using a template), resulting product pool contaminated with longer-than-desired, (partially) double-stranded impurities. Current methods often fail to fully eliminate these impurities, which, if...
RNAs are synthesized exclusively in a 5′ to 3′ direction and it is well established that many (particularly long noncoding, but also riboswitch) begin fold before the transcript fully sy...