A5031710347- Wastewater Treatment and Nitrogen Removal
- Microbial Community Ecology and Physiology
- Microbial Fuel Cells and Bioremediation
- Genomics and Phylogenetic Studies
- Constructed Wetlands for Wastewater Treatment
- Biofuel production and bioconversion
- Marine and coastal ecosystems
- Water Treatment and Disinfection
- Microbial Metabolic Engineering and Bioproduction
- Enzyme Catalysis and Immobilization
Universität Hamburg
2012-2023
University of Göttingen
2011
The cohort of the ammonia-oxidizing archaea (AOA) phylum Thaumarchaeota is a diverse, widespread and functionally important group microorganisms in many ecosystems. However, our understanding their biology still very rudimentary part because all available genome sequences this are from members Nitrosopumilus cluster. Here we report on complete sequence Candidatus Nitrososphaera gargensis obtained an enrichment culture, representing different evolutionary lineage AOA frequently found high...
Nitrification has an immense impact on nitrogen cycling in natural ecosystems and wastewater treatment plants. Mathematical models function as tools to capture the complexity of these biological systems, but kinetic parameters especially nitrite-oxidizing bacteria (NOB) are lacking because a limited number pure cultures until recently. In this study, we compared nitrite oxidation kinetics six one enrichment culture representing three genera NOB (Nitrobacter, Nitrospira, Nitrotoga). With...
Nitrospira are the most widespread and diverse known nitrite-oxidizing bacteria key nitrifiers in natural engineered ecosystems. Nevertheless, their ecophysiology environmental distribution understudied because of recalcitrance to cultivation lack a molecular functional marker, which would allow detection environment. Here we introduce nxrB, gene encoding subunit beta nitrite oxidoreductase, as phylogenetic marker for Nitrospira. Phylogenetic trees based on nxrB were largely congruent 16S...
In marine systems, nitrate is the major reservoir of inorganic fixed nitrogen. The only known biological nitrate-forming reaction nitrite oxidation, but despite its importance, our knowledge organisms catalyzing this key process in N-cycle very limited. most frequently encountered NOB are related to Nitrospina gracilis, an aerobic chemolithoautotrophic bacterium isolated from ocean surface waters. To date, limited physiological and genomic data for organism were available phylogenetic...
Microbial metagenomes derived from soils are rich sources for the discovery of novel genes and biocatalysts. Fourteen environmental plasmid seven fosmid libraries obtained 10 German forest (A horizons) six grassland B were screened conferring lipolytic activity. The comprised approximately 29.3 Gb cloned soil DNA. Partial activity-based screening constructed resulted in identification 37 unique clones. amino acid sequences corresponding gene products shared 29-90% identity to other enzymes,...
Nitrite-oxidizing bacteria (NOB) have conventionally been regarded as a highly specialized functional group responsible for the production of nitrate in environment. However, recent culture-based studies suggest that they capacity to lead alternative lifestyles, but direct environmental evidence contribution marine nitrite oxidizers other processes has lacking date. We report on biogeochemical functions, worldwide distribution, and sometimes high abundance NOB
The second step of nitrification, the oxidation nitrite to nitrate, is vital for functioning nitrogen cycle, but our understanding ecological roles involved microorganisms still limited. known diversity Nitrospira, most widely distributed nitrite-oxidizing bacteria, has increased remarkably by analyses 16S rRNA and functional gene sequences. However, only few representatives could be brought into laboratory cultures so far. In this study, two Nitrospira from activated sludge were isolated...
Nitrification is an essential process for N removal in activated sludge to avoid toxicity of ammonium and nitrite. Besides Nitrospira, "Candidatus Nitrotoga" has been identified as a key nitrite-oxidizing bacterium (NOB) performing the second step nitrification, nitrite oxidation nitrate, wastewater treatment plants (WWTPs). However, driving forces dominance Nitrotoga certain have often remained unclear could not be explained solely by temperature effects. In this study, we characterized...
AME Aquatic Microbial Ecology Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout JournalEditorsSpecials 80:289-300 (2017) - DOI: https://doi.org/10.3354/ame01859 Oxidation kinetics and inverse isotope effect of marine nitrite-oxidizing isolates Juliane Jacob1,2,*, Boris Nowka3, Véronique Merten4, Tina Sanders1, Eva Spieck3, Kirstin Dähnke1 1Helmholtz Center Geesthacht, Institute Coastal Research, Max-Planck-Str. 1, 21502...
The genus Nitrospira is considered to be the most widespread and abundant group of nitrite-oxidizing bacteria in many natural man-made ecosystems. However, ecophysiological versatility within this phylogenetic remains highly understudied, mainly due lack pure cultures genomic data. To further expand our understanding biotechnologically important genus, we analyzed high quality draft genome "Nitrospira lenta" strain BS10, a sublineage II that was isolated from municipal wastewater treatment...
Abstract The genus Nitrospira represents the dominant nitrite-oxidizing clade in most wastewater treatment plants (WWTPs) globally, and several strains have been isolated from activated sludge. Using a pre-enrichment strategy with alternating nitrifying denitrifying conditions, followed by incubation at elevated temperatures, we novel species, named tepida. This moderately thermophilic species optimal growth between 37 45°C is only distantly related to other forms lineage VII within genus,...