- Plant Virus Research Studies
- Bacteriophages and microbial interactions
- Advanced biosensing and bioanalysis techniques
- Biosensors and Analytical Detection
- Transgenic Plants and Applications
- Legume Nitrogen Fixing Symbiosis
- Plant and Fungal Interactions Research
- Plant Disease Resistance and Genetics
- Advanced Biosensing Techniques and Applications
- Plant Parasitism and Resistance
- RNA Interference and Gene Delivery
- Electrochemical sensors and biosensors
- Nanowire Synthesis and Applications
- Plant-Microbe Interactions and Immunity
- Analytical Chemistry and Sensors
- Insect-Plant Interactions and Control
- Monoclonal and Polyclonal Antibodies Research
- Viral gastroenteritis research and epidemiology
- Nanopore and Nanochannel Transport Studies
- Force Microscopy Techniques and Applications
- Modular Robots and Swarm Intelligence
- Diatoms and Algae Research
- Nanofabrication and Lithography Techniques
- Acoustic Wave Resonator Technologies
- Characterization and Applications of Magnetic Nanoparticles
University of Stuttgart
2015-2024
Stuttgart Observatory
2018
Krill (Czechia)
2010
Christ University
2010
Max Planck Institute of Microstructure Physics
2006
Max Planck Institute for Solid State Research
2003
Large biomolecules are attractive templates for the synthesis of metal1-7 and inorganic8-10 compound nanostructures. The well-defined chemical structural heterogeneity biotemplates can be exploited precise control size shape formed Here, we demonstrate that central channel tobacco mosaic virus (TMV) used as a template to synthesize nickel cobalt nanowires only few atoms in diameter, with lengths up micrometer range.
Abstract Tobacco mosaic virus (TMV) is a very stable nanotube complex of helical RNA and 2130 coat proteins. The special shape makes it an interesting nano‐object, especially as template for chemical reactions. Here we use TMV chemically functionalized binding metal ions. Different groups the protein can be used ligands or to electrostatically bind Following this activation step, reduction electroless plating produces clusters several nanometers in diameter. are attached virion without...
Decoration of nanoparticles, in particular biomolecules, gathered high attention recent years.1-7 Of special interest is the potential use biomolecules as templates for fabrication semiconducting or metallic nanostructures.1-7,26 In this work we show application atomic layer deposition, a gas-phase thin film deposition process, to biological macromolecules, which are frequently used nanoscale science, and possibility fabricate metal oxide nanotubes films with embedded biomolecules.1-13
The size and shape of nanocarriers can affect their fate in vivo, but little is known about the effect nanocarrier aspect ratio on biodistribution setting cancer imaging drug delivery. production nanoscale anisotropic materials a technical challenge. A unique biotemplating approach based rod‐shaped nucleoprotein nanoparticles with predetermined ratios (AR 3.5, 7, 16.5) used. These rigid, soft‐matter nanoassemblies are derived from tobacco mosaic virus (TMV) components. role nanoparticle...
The spacing of functional nanoscopic elements may play a fundamental role in nanotechnological and biomedical applications, but is so far rarely achieved on this scale. In study we show that tobacco mosaic virus (TMV) the RNA-guided self-assembly process its coat protein (CP) can be used to establish new nanorod scaffolds loaded not only with homogeneously distributed functionalities, distinct molecule species grouped ordered along longitudinal axis. arrangement resulting domains final...
A bioinspired approach to the fabrication of nanostructured functional devices is described. ZnO nanowires are formed by self-assembly nanosized building blocks controlled tobacco mosaic virus (TMV) templates at nearly ambient conditions. This process allows integration TMV/ZnO nanostructures into field-effect transistors (FETs) with prestructured electrodes. The FETs operate as-deposited, without further treatment. Detailed facts importance specialist readers published as "Supporting...
Tobacco mosaic virus (TMV) is a robust nanotubular nucleoprotein scaffold increasingly employed for the high density presentation of functional molecules such as peptides, fluorescent dyes and antibodies. We report on its use advantageous carrier sensor enzymes. A TMV mutant with cysteine residue exposed every coat protein (CP) subunit (TMVCys) enabled coupling bifunctional maleimide-polyethylene glycol (PEG)-biotin linkers (TMVCys/Bio). Its surface was equipped two streptavidin...
ABSTRACT Since 1997 two distinct geminivirus species, Tomato yellow leaf curl Sardinia virus (TYLCSV) and (TYLCV), have caused a similar disease in tomato, coexisted the fields of southern Spain, very frequently doubly infected single plants. Tomatoes as well experimental test plants (e.g., Nicotiana benthamiana ) showed enhanced symptoms upon mixed infections under greenhouse conditions. Viral DNA accumulated to extent singly In situ tissue hybridization TYLCSV TYLCV DNAs be confined phloem...
We studied the adsorption behavior and surface chemistry of tobacco mosaic virus (TMV) on well-defined metal insulator surfaces. TMV serves as a tubular supramolecular model system with precisely known termination. show that if substrate pH-dependent molecular match, for example, by hydrogen bonding, strong occurs, lateral movement is impeded. Due to immobilization, virion can be imaged atomic force microscopy (AFM) in contact mode. also used self-assembled monolayers an acyl chloride group...
ABSTRACT Geminiviruses manage the transport of their DNA within plants with help three proteins, coat protein (CP), nuclear shuttle (NSP), and movement (MP). The DNA-binding capabilities CP, NSP, MP Abutilon mosaic virus (AbMV; family Geminiviridae ; genus Begomovirus ) were scrutinized using gel mobility shift assays electron microscopy. CP NSP revealed a sequence-independent affinity for both double-stranded single-stranded DNA, as has been previously reported other begomoviruses....
Applying a magnetic field to many ferrofluids leads significant increase in viscosity, but the phenomenon has yet find technological exploitation because of thinning caused by even weak shear flows. We have discovered that addition plant-virus-derived nanotubes commercial ferrofluid can give rise dramatic enhancement magnetoviscosity and suppression thinning. The dependence this effect on nanotube aspect ratio surface charge, both which were varied biotechnologically, is consistent with...
The DNA origami method produces programmable nanoscale objects that form when one long scaffold strand hybridizes to numerous oligonucleotide staple strands. One is dominating the field: M13mp18, a bacteriophage-derived vector 7249 nucleotides in length. full-length M13 typically folded by using over 200 oligonucleotides. Here we report convenient preparation of 704 nt fragment dubbed "M1.3" as linear or cyclic and assembly small structures with just 15-24 A typical M1.3 large enough be...
We report the binding of nanoparticles (NPs) to wild type (unmodified) tobacco mosaic virus (TMV). The viruses are simply mixed with citrate-coated, negatively charged gold and iron oxide (IONPs) in acidic solution. This results TMV decorated along its whole length by respective particles. Such a decoration usually requires chemical modification or mutation (e.g., cysteine residues), but here we reduce TMV's natural negative charge protonation. particles protonated much smaller extent....