A5018225466- Neuroscience and Neuropharmacology Research
- Receptor Mechanisms and Signaling
- Pain Mechanisms and Treatments
- Adipose Tissue and Metabolism
- Neurotransmitter Receptor Influence on Behavior
- Photoreceptor and optogenetics research
- Neural dynamics and brain function
- Sleep and Wakefulness Research
- Neuroendocrine regulation and behavior
- Memory and Neural Mechanisms
- Maternal Mental Health During Pregnancy and Postpartum
- Anesthesia and Neurotoxicity Research
- Neuropeptides and Animal Physiology
- Vestibular and auditory disorders
- Radioactive element chemistry and processing
- Radioactivity and Radon Measurements
- Chemical synthesis and alkaloids
- Acupuncture Treatment Research Studies
- Ophthalmology and Eye Disorders
- Cardiovascular Syncope and Autonomic Disorders
- Stress Responses and Cortisol
- Radioactive contamination and transfer
- Protein Interaction Studies and Fluorescence Analysis
- Nicotinic Acetylcholine Receptors Study
- Pharmacological Effects and Assays
Washington University in St. Louis
1969-2024
University of Health Sciences and Pharmacy
2023-2024
National Taiwan University
2020-2022
The locus coeruleus (LC) contains noradrenergic (NA) neurons that respond to novel stimuli in the environment with phasic activation initiate an orienting response; LC is also triggered by stimuli, representing outcome of task-related decision processes, facilitate ensuing behaviours and help optimize task performance. Here, we report LC-NA exhibit bursts action potentials vitro resembling vivo, activity gated inhibitory interneurons (I-INs) located peri-LC. We observe inhibition peri-LC...
Selective manipulation of neural circuits using optogenetics and chemogenetics holds great translational potential but requires genetic access to neurons. Here, we demonstrate a general framework for identifying tool-independent, pharmacological strategies circuit-selective modulation. We developed an economically accessible calcium imaging-based approach large-scale scans endogenous receptor-mediated activity. As testbed this approach, used the mouse locus coeruleus due combination its...
Early life stress (ELS) in humans and preclinical rodent models profoundly impacts the brain correlates with negative affective behaviors adulthood. The locus coeruleus (LC), a stress-responsive brainstem nucleus that supplies most of norepinephrine (NE), is known to modulate affect. Here we used repeated maternal separation (MSS) investigate impact ELS on LC stress-related Using ex vivo cell-attached electrophysiology, recorded spontaneous firing across lifespan from early development,...
Abstract The brainstem region, locus coeruleus (LC), has been remarkably conserved across vertebrates. Evolution woven the LC into wide-ranging neural circuits that influence functions as broad autonomic systems, stress response, nociception, sleep, and high-level cognition among others. Given this conservation, there is a strong possibility activity inherently similar species, furthermore age, sex, brain state similarly species. degree to which homogenous these factors, however, never...
Psychedelics hold therapeutic promise for mood disorders due to rapid, sustained results. Human neuroimaging studies have reported dramatic serotonin-2A receptor-(5-HT2AR)-dependent changes in functional brain reorganization that presumably reflect neuromodulation. However, the potent vasoactive effects of serotonin been overlooked. We found psilocybin-mediated alterations fMRI-HRFs humans, suggesting potentially altered NVC. To assess neuronal, hemodynamic, and neurovascular coupling (NVC)...
Uranium concentrations and distributions in separated clinopyroxenes of eclogites from four different sites have been studied by the fission track method. Types uranium occurrences included (i) inhomogeneously distributed clusters, (ii) line at cracks, (iii) uniform body mineral. Different grains same sample generally vary factors 2 to 4 uniformly concentration. Samples locations their average concentration a factor approximately 1000 (that is, 0.01 20 parts per billion uranium). The degree...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSurface-enhanced Raman scattering of adenosine triphosphate moleculesT. T. Chen, C. S. Kuo, Y. Chou, and N. LiangCite this: Langmuir 1989, 5, 4, 887–891Publication Date (Print):July 1, 1989Publication History Published online1 May 2002Published inissue 1 July 1989https://pubs.acs.org/doi/10.1021/la00088a001https://doi.org/10.1021/la00088a001research-articleACS PublicationsRequest reuse permissionsArticle Views328Altmetric-Citations21LEARN ABOUT...
Age-associated reduced motivation is a hallmark of neuropsychiatric disorders in the elderly. In our rapidly aging societies, it critical to keep levels high enough promote healthspan and lifespan. However, how during remains unknown. Here, we used multiple mouse models evaluate related affective states young old mice. We also compared effect social isolation, common stressor, those aging. found that both isolation decreased mice, but Bdnf expression ventral tegmental area (VTA) was...
Abstract The norepinephrine-releasing neurons in the locus coeruleus (LC) are well known to regulate wakefulness/arousal. They display active firing during wakefulness and a decreased discharge rate sleep. We have previously reported that LC express large numbers of GABA B receptors (GABA Rs) located at peri-/extrasynaptic sites subject tonic inhibition due continuous activation Rs by ambient GABA, which is significantly higher sleep than wakefulness. In this study, we further showed using...
The endogenous opioid peptide systems are critical for analgesia, reward processing, and affect, but research on their release dynamics function has been challenging. Here, we have developed microimmunoelectrodes (MIEs) the electrochemical detection of peptides using square-wave voltammetry. Briefly, a voltage is applied to electrode cause oxidation tyrosine residue interest, which detected as current. To provide selectivity these voltammetric measurements, carbon fiber surface MIE coated...
Abstract The locus coeruleus (LC) plays a paradoxical role in chronic pain. Although largely known as potent source of endogenous analgesia, increasing evidence suggests injury can transform the LC into pain generator. We sought to clarify this system Here, we show optogenetic inhibition activity is acutely antinociceptive. Following long-term spared nerve injury, same analgesic – further supporting its generator function. To identify inhibitory substrates that may naturally serve function,...
Distinct excitatory synaptic inputs to the locus coeruleus (LC) modulate behavioral flexibility. Here we identify a novel monosynaptic glutamatergic input LC from ventral tegmental area (VTA). We show robust VTA axonal projections provide direct transmission LC. Despite weak summation, optogenetic activation of these axons enhances tonic firing and facilitates real-time conditioned aversive behaviors. hypothesized this projection may integration with other inputs. then used coincident VTA-LC...
The locus coeruleus (LC) consists of noradrenergic (NA) neurons and plays an important role in controlling behaviours. Although much the knowledge regarding LC functions comes from studying behavioural outcomes upon administration muscarinic acetylcholine receptor (mAChR) agonists into nucleus, exact mechanisms remain unclear. Here, we report that application carbachol (CCh), mAChR agonist, increased spontaneous action potentials (sAPs) both LC-NA local inhibitory interneurons (LC I-INs)...
<b>Abstract ID 24694</b> <b>Poster Board 346</b> The locus coeruleus (LC) provides the largest noradrenergic innervation to central nervous system and is a major node in pain neural circuitry. LC neurons directly project dorsal horn of spinal cord. It through these direct projections leverage their antinociceptive influence locally via alpha-2 adrenoreceptor activation inhibit nociceptive afferents. responds noxious stimuli with shift from low tonic phasic stimulation has been shown be...
<b>Abstract ID 23754</b> <b>Poster Board 169</b> The noradrenergic neurons in locus coeruleus (LC) are the main source of norepinephrine (NE) for central nervous system. Potential modular organization LC could provide functional differentiation during distinct behaviors. This would suggest that different modules should act differentially according to behavioral state. is enriched many G-protein coupled receptors (GPCRs) likely endogenously activated behavior regulate activity among modules,...