A5091562162- Integrated Circuits and Semiconductor Failure Analysis
- DNA Repair Mechanisms
- PARP inhibition in cancer therapy
- Parathyroid Disorders and Treatments
- bioluminescence and chemiluminescence research
- Genetic and Kidney Cyst Diseases
- Genetic Syndromes and Imprinting
- Photoreceptor and optogenetics research
- Advanced Fluorescence Microscopy Techniques
Leibniz Institute on Aging - Fritz Lipmann Institute (FLI)
2019-2024
University of Isfahan
2017
Poly(ADP-ribose) polymerase-1 (PARP1) binds DNA lesions to catalyse poly(ADP-ribosyl)ation (PARylation) using NAD+ as a substrate. PARP1 plays multiple roles in cellular activities, including repair, transcription, cell death, and chromatin remodelling. However, whether these functions are governed by the enzymatic activity or scaffolding function of remains elusive. In this study, we inactivated mice truncating its C-terminus that is essential for ART catalysis (PARP1ΔC/ΔC, designated...
Renilla Luciferase (RLuc) is a blue light emitter protein which can be applied as valuable tool in medical diagnosis. But due to lack of the crystal structure RLuc‐ligand complex, functional motions and catalytic mechanism this enzyme remain largely unknown. In present study, active site properties ligand‐receptor interactions native RLuc its red‐shifted emitting variant (Super 8) were investigated using molecular docking approach, dynamics (MD) analysis, MM‐PBSA method. The detailed...
Klotho is an anti-aging protein whose deletion significantly reduces lifespan in mice, while its over-expression increases lifespan. a type-I transmembrane that N-glycosylated at eight positions within ectodomain. Our study demonstrates N-glycosylation or mutation position N614, but not N161, N285, N346 mouse Klotho, critically involved the transport of out endoplasmic reticulum (ER). Consequently, wild-type Klotho-EGFP as well mutants N161Q, N285Q, and N346Q were present plasma membrane...