A5030547034- Neuroscience and Neuropharmacology Research
- Neonatal Respiratory Health Research
- Memory and Neural Mechanisms
- Neural dynamics and brain function
- Photoreceptor and optogenetics research
- Tissue Engineering and Regenerative Medicine
- Emergency and Acute Care Studies
- Streptococcal Infections and Treatments
- Cell death mechanisms and regulation
- Cholinesterase and Neurodegenerative Diseases
- Neuroscience and Neural Engineering
- Congenital Diaphragmatic Hernia Studies
- Asthma and respiratory diseases
- Nerve injury and regeneration
- NF-κB Signaling Pathways
- Mesenchymal stem cell research
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Pneumonia and Respiratory Infections
- Congenital heart defects research
- Cell Adhesion Molecules Research
- TGF-β signaling in diseases
- Nicotinic Acetylcholine Receptors Study
- Infective Endocarditis Diagnosis and Management
- Neurogenesis and neuroplasticity mechanisms
- Respiratory viral infections research
Boston University
2015-2025
Dalhousie University
2010-2025
Harvard University
2013-2025
Boston Children's Hospital
2016-2025
Boston Children's Museum
2013-2024
Kingston University
2022
Pulmonary Associates
2000-2019
University School
2007-2019
Roxbury Community College
2016-2019
VA Boston Healthcare System
2018
We assessed the capacity of plastic-adherent cultured bone marrow cells to serve as precursors differentiated parenchymal lung. By intravenously delivering lacZ-labeled into wild-type recipient mice after bleomycin-induced lung injury, we detected marrow-derived engrafted in parenchyma with morphological and molecular phenotype type I pneumocytes alveolar epithelium. At no time cell injection, did detect any engraftment II pneumocytes. In addition, found that fresh aspirates can express...
Heart failure remains a leading cause of morbidity and mortality. The cellular mechanism underlying the development cardiac dysfunction is decrease in number viable cardiomyocytes. Recent observations have suggested that adult heart may contain progenitor cell population. Side population (SP) cells, characterized by distinct Hoechst dye efflux pattern, been shown to exist multiple tissues are capable tissue-specific differentiation. In this report, we confirm existence SP population,...
We examined the effects of transforming growth factor-beta (TGF-beta) on production collagen by cultures human embryonic lung fibroblasts. TGF-beta at 0.1 ng/ml appeared to activate selectively extracellular accumulation as compared with total protein production. A maximal effect inducing a 2-3-fold increase in and occurred dose 1.0 fibroblast cultures. had no proliferation after 24- 48-h exposure, including that received second 24 h. Collagenase digestion radiolabeled derived from...
<h3>Background</h3>The Centor and McIsaac scores guide testing treatment for group A streptococcal (GAS) pharyngitis in patients presenting with a sore throat, but they were derived on relatively small samples. We perform national-scale validation of the prediction models large, geographically diverse population.<h3>Methods</h3>We analyzed data collected from 206 870 3 years or older who presented painful throat to United States national retail health chain September 1, 2006, December 2008....
Abstract Background Diagnostic test evaluation requires a reference standard. We describe an approach for creating standard acute infection using unrestricted adjudication and apply it to compare biomarker tools. Methods Adults children with suspected enrolled in three prospective studies at emergency departments urgent cares were included. Adjudicators, blinded C-reactive protein, procalcitonin, MeMed BV (MMBV), labeled each case (bacterial/viral/non-infectious/indeterminate). Initial...
Acetylcholine (ACh) is an important neurotransmitter in the mammalian brain; it implicated arousal, learning, and other cognitive functions. Recent studies indicate that nicotinic receptors contribute to these cholinergic effects, addition established role of muscarinic receptors. In hippocampus, where involvement learning memory particularly well documented, α7 acetylcholine receptor subunits (α7 nAChRs) are highly expressed, but their precise ultrastructural localization has not been...
Side population (SP) cells are a rare subset of found in various tissues that highly enriched for stem cell activity. SP can be isolated by dual-wavelength flow cytometry because their capacity to efflux Hoechst dye, process mediated the ATP-binding cassette transporter breast cancer resistance protein (Bcrp) 1. By performing enzymedigested mouse lung stained with we comprise 0.03–0.07% total and evenly distributed proximal distal regions. RT-PCR, express hepatocyte nuclear factor-3β, but...
Tissue-specific progenitor cells contribute to local cellular regeneration and maintain organ function. Recently, we have determined that cardiac side-population (CSP) represent a distinct cell population, capable of in vitro differentiation into functional cardiomyocytes. The response endogenous CSP myocardial injury, however, the mechanisms this pool vivo remain unknown. In report demonstrate proliferation maintains under physiologic conditions, with little contribution from extracardiac...
We examined the effects of insulin and insulin-like growth factor I (IGF-I) on production collagen by cultures human embryonic lung fibroblasts. Insulin at 20 ng/ml increased accumulation 58% total protein formation 18%. At 2 micrograms/ml, 2- to 3-fold 2-fold. The mRNA levels for alpha 1(I) 1(III) chains were elevated compared with untreated control values. IGF-I 10 100 maximally 3-fold. A specific antibody receptor (alpha IR-3) caused a concentration-related decline in insulin-induced...
Under some conditions, synaptically released glutamate can exert long-range actions in the cortical microcircuitry. To what extent spillover leads to direct cross talk among individual synapses remains unclear. We recorded NMDAR-mediated EPSCs acute hippocampal slices at 35°C by stimulating two independent pathways that converge on same CA1 pyramidal cell. Activation of a conditioning pathway presence use-dependent blocker dizocilpine maleate (MK801) resulted partial NMDA receptor (NMDAR)...
Epithelial cells are considered to be a main target of bleomycin-induced lung injury, which leads fibrosis in vivo. We studied the characteristics vitro apoptosis mouse epithelial (MLE) cell line. Bleomycin caused an increase reactive oxygen species (ROS) resulting oxidative stress, mitochondrial leakage, and apoptosis. These were associated with elevated caspase-8 resultant caspase-9 activity upregulation Fas expression. Glutathione inhibitors or caspase-9, but not FasL, inhibited these...
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The memory dysfunction of Alzheimer disease has been associated with a cortical cholinergic deficiency and loss neurons the nucleus basalis Meynert. This component can be modeled in rat by ibotenic acid lesions magnocellularis. impairment caused such unilateral lesions, as reflected passive avoidance behavior, is reversed grafts into deafferented neocortex embryonic ventral forebrain, but not noncholinergic hippocampal cells.
Using confocal microscopy in conjunction with microdrop application of Dil, we have imaged and measured individual dendritic spines living hippocampal CA1 pyramidal neurons acute brain slices, before approximately 3 hr after induction long-term potentiation by chemical means. Statistical analysis changes the length spines, comparison results Monte Carlo simulations, suggests that two forms structural change occur chemically induced potentiation: growth a subpopulation small angular...
To further examine the half-life of alveolar macrophages, chimeric CD 45.2 mice were generated through bone marrow transplantation donor 45.1 cells. Before administration cells, recipient divided into two cohorts: first cohort received total body irradiation; second also irradiation-however, thorax, head, and upper extremities shielded with lead. Flow cytometric analysis was then performed on blood, peritoneal, bronchoalveolar lavage cells over time to quantify engraftment. The data for...
Abstract Calmodulin-based genetically encoded fluorescent calcium indicators (GCaMP-s) are powerful tools of imaging dynamics from cells to freely moving animals. High affinity with slow kinetics however distort the temporal profile transients. Here we report development reduced ultrafast variants GCaMP6s and GCaMP6f. We hypothesized that GCaMP-s have a common kinetic mechanism rate-limiting process in interaction RS20 peptide calcium-calmodulin. Therefore targeted specific residues binding...